Introduction to animal cell culture technology—past, present and future

Merten, Otto‐Wilhelm

doi:10.1007/s10616-006-9009-4

Cited by 48 publications

(39 citation statements)

References 44 publications

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“…In 50s and 60s, another diploid cell lines were developed-HeLa (by Gay, see subsection 7) MRC-5 (by Jacobs) and WI-38 (by Hayflick and Moorhead) from human tissue and Vero (Verde-French for green and RenO-French for kidney) cell line obtained from simian tissue [70,74]. The examples of the earliest derived cell lines are presented in Table 1.…”

Section: Cell Line Cultures Developmentmentioning

confidence: 99%

“…Moreover, human cell cultures can also be used for personal therapies-gene therapies, tissue engineering, transplantation of organs. In the future, more human diseases will be treated by new form of therapies based on organ and tissue cultures [74].…”

Section: Future Of Cell Culturesmentioning

confidence: 99%

“…Future medicine will able to use widely stem cells [adult and as well human embryonic stem cells (HESC)] for damage tissue replacement [74]. The idea of Dr. Atala's of in situ bioprinting therapy and the results obtained in that area are promising.…”

Section: Future Of Cell Culturesmentioning

confidence: 99%

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History of Cell Culture

Jędrzejczak-Silicka¹

2017

New Insights Into Cell Culture Technology

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From the ancient Romans, through the Middle Ages, to the late of the nineteenth century, the Aristotelian doctrine of spontaneous generation was one of the most basic laws. Even the invention of the microscope and investigations of Leeuwenhoek and Hook did not disprove the Aritostelian doctrine. Finally, in the eighteenth century, the spontaneous generation doctrine was laid by Louis Pasteur. Moreover, in the first decade of the eighteenth century, nucleus was observed in plant and animal tissues, and Virchow and other scientists presented the view that cells are formed via scission of preexisting cells. In the first decade of the twentieth century, Ross Harrison developed the first techniques of cell culture in vitro, and Burrows and Carrel improved Harrison's cell cultures. In mid-twentieth century, the basic principles for plant and animal cell cultures in vitro were developed, and human diploid cell lines were established. On the basis of knowledge about the cell cycle and gene expression regulation, the first therapeutic proteins were produced using mammalian cell cultures. The end of twentieth century and early twenty-first century brought the progress in 3-D cell culture technology and created the possibility of the tissue engineering and the regenerative medicine development.

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Section: Cell Line Cultures Developmentmentioning

confidence: 99%

Section: Future Of Cell Culturesmentioning

confidence: 99%

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History of Cell Culture

Jędrzejczak-Silicka¹

2017

New Insights Into Cell Culture Technology

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“…Several glycoproteins and monoclonal antibodies with relatively high production needs are being identified as new drug candidates (Walsh, 2006). The Chinese Hamster Ovary (CHO) cell line is one of the most used for obtaining biological therapeutics (Merten, 2006).…”

Section: Introductionmentioning

confidence: 99%

Preliminary Modeling of an Industrial Recombinant Human Erythropoietin Purification Process by Artificial Neural Networks

Garcel

León

Magaz

2015

Braz. J. Chem. Eng.

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-In the present study a preliminary neural network modelling to improve our understanding of Recombinant Human Erythropoietin purification process in a plant was explored. A three layer feed-forward back propagation neural network was constructed for predicting the efficiency of the purification section comprising four chromatographic steps as a function of eleven operational variables. The neural network model performed very well in the training and validation phases. Using the connection weight method the predictor variables were ranked based on their estimated explanatory importance in the neural network and five input variables were found to be predominant over the others. These results provided useful information showing that the first chromatographic step and the third chromatographic step are decisive to achieve high efficiencies in the purification section, thus enriching the control strategy of the plant.

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“…Recombinant Chinese hamster ovary (CHO) cells are widely applied in biopharmaceutical industries for the production of various glycoprotein therapeutics (Merten, 2006). Serum is a rich source of different components such as growth factors, proteins, vitamins, trace elements and hormones essential for the survival and growth of cells (Riebeling et al 2011).…”

Section: Introductionmentioning

confidence: 99%

Rational development of a serum-free medium and fed-batch process for a GS-CHO cell line expressing recombinant antibody

et al. 2012

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A serum-free medium (CHO-SFM) together with a fed-batch process was developed for the cultivation of a recombinant GS-CHO cell line producing TNFR-Fc. According to the metabolic characteristics of GS-CHO cell, a basal medium was prepared by supplementing DMEM:F12:RPMI1640 (2:1:1) with amino acids, insulin, transferrin, Pluronic F68 and some other ingredients. Statistical optimization approaches based on Plackett-Burman and central composite designs were then adopted to identify additional positive determinants and determine their optimal concentrations, which resulted in the final CHO-SFM medium formulations. The maximum antibody titer reached was 90.95 mg/l in the developed CHO-SFM, which was a 18 % and 10 fold higher than that observed in the commercial EX-CELL TM 302 medium (76.95 mg/l) and basal medium (8.28 mg/l), respectively. Subsequently, a reliable, reproducible and robust fed-batch strategy was designed according to the offline measurement of glucose, giving a final antibody yield of 378 mg/l, which was a threefold improvement over that in conventional batch culture (122 mg/l) using CHO-SFM. In conclusion, the use of design of experiment (DoE) method facilitated the development of CHO-SFM medium and fed-batch process for the production of recombinant antibody using GS-CHO cells.

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Introduction to animal cell culture technology—past, present and future

Cited by 48 publications

References 44 publications

History of Cell Culture

History of Cell Culture

Preliminary Modeling of an Industrial Recombinant Human Erythropoietin Purification Process by Artificial Neural Networks

Rational development of a serum-free medium and fed-batch process for a GS-CHO cell line expressing recombinant antibody

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