Introduction to magnetic resonance methods in photosynthesis

Huber, Martina

doi:10.1007/s11120-009-9442-2

Cited by 5 publications

(2 citation statements)

References 16 publications

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“…It is shown that three atoms (D···O • –N) lie on the same plane. Here, we used the following assumption: the g tensor of TEMPO-MI is same with that of other TEMPO derivative compounds; the g zz axis is parallel to the p z orbital of nitroxide radical and the g xx axis is parallel to the N–O • direction. ,− Interestingly, 2 H ENDOR spectra of 1 , 2 , and 3 are similar and are not affected by the concentration of salt (Figure S6). This phenomenon may imply that the hydrogen bonding networks are unchanged by the degree of self-assembly events and are very well conserved at the initial stage of self-assembly.…”

Section: Results and Discussionmentioning

confidence: 99%

Investigation of the Hydration State of Self-Assembled Peptide Nanostructures with Advanced Electron Paramagnetic Resonance Spectroscopy

et al. 2019

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Probing the intermolecular interactions and local environments of self-assembled peptide nanostructures (SPNs) is crucial for a better understanding of the underlying molecular details of self-assembling phenomena. In particular, investigation of the hydration state is important to understand the nanoscale structural and functional characteristics of SPNs. In this report, we examined the local hydration environments of SPNs in detail to understand the driving force of the discrete geometric structural self-assembling phenomena for peptide nanostructures. Advanced electron paramagnetic resonance spectroscopy was used to probe the hydrogen bond formation and geometry as well as the hydrophobicity of the local environments at various spin-labeled sites in SPNs. The experimental results supplement the sparse experimental data regarding local structures of SPNs, such as the hydrogen bonding and the hydrophobicity of the local environment, providing important information on the formation of SPNs, which have immense potential for bioactive materials.

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Section: Results and Discussionmentioning

confidence: 99%

Investigation of the Hydration State of Self-Assembled Peptide Nanostructures with Advanced Electron Paramagnetic Resonance Spectroscopy

et al. 2019

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“…24 Protonophores assist in dissipating this membrane potential barrier. 25 Electron paramagnetic resonance studies of PSI 26,27 had indirectly implied the probable influence of SMF on the cyclic electron flow around PSI. [28][29][30] PSI is one of two multi-subunit pigment-protein complexes embedded in the thylakoids of chloroplast and the inner membranes of cyanobacteria.…”

Section: Introductionmentioning

confidence: 99%

Static magnetic field (SMF) sensing of the P723/P689 photosynthetic complex

Bhattacharya

Chakraborty

Raja

et al. 2014

Photochem Photobiol Sci

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Moderate intensity SMF have been shown to act as a controller of the protic potential in the coherent milieu of the thylakoid membranes. SMF of the order of 60-500 mT induces memory-like effect in photosystem I (PSI, P723) emission with a correlated oscillation of photosystem II (PSII, P689) fluorescence emission at a temperature of 77 K. The observed magnetic perturbation that affects the thylakoid photon capture circuitry was also found to be associated with the bio-energetic machinery of the thylakoid membranes. At normal pH, SMF causes an enhancement of PSI fluorescence emission intensity (P723/P689 > 1), followed by a slow relaxation on the removal of SMF. The enhancement of the PSI fluorescence intensity also occurs under no-field condition, if either the pH of the medium is lowered, or protonophores, such as carbonyl cyanide chlorophenylhydrazine or nigericin are added (P723/P689≥ 2). If SMF was applied under such a low pH condition or in the presence of protonophore, a reverse effect, particularly, a reduction of the enhanced PSI emission was observed. Because SMF is essentially equivalent to a spin perturbation, the observed effects can be explained in terms of spin re-organization, illustrating a memory effect via membrane re-alignment and assembly. The mimicry of conventional uncouplers by SMF is also notable; the essential difference being the reversibility and manoeuvrability of the latter (SMF). Finally, the effect implies numerous possibilities of externally regulating the photon capture and proton circulation in the thylakoid membranes using controlled SMF.

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EPR Spectroscopy of Nitroxide Spin Probes

Bordignon

2017

eMagRes

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Introduction to magnetic resonance methods in photosynthesis

Cited by 5 publications

References 16 publications

Investigation of the Hydration State of Self-Assembled Peptide Nanostructures with Advanced Electron Paramagnetic Resonance Spectroscopy

Investigation of the Hydration State of Self-Assembled Peptide Nanostructures with Advanced Electron Paramagnetic Resonance Spectroscopy

Static magnetic field (SMF) sensing of the P723/P689 photosynthetic complex

EPR Spectroscopy of Nitroxide Spin Probes

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