Introduction to Special Issue on “Fluorescence-Based Sensing Technologies”

Holmes-Smith, A. Sheila

doi:10.3390/bios5040616

Cited by 5 publications

(3 citation statements)

References 5 publications

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“…Certainly, other various techniques have been developed, including electrochemical sensor, surface plasmon resonance (SPR), surfaceenhanced Raman scattering (SERS), colorimetry and uorescence [10][11][12][13][14]. Among the various techniques, uorescence detection platform has emerged as an excellent alternative for the detection, quanti cation and characterization of target [15][16][17]. Organic uorescent dye-labeled DNA probe and uorescent technique have been widely employed for ultrasensitive quanti cation of DNA, miRNA, proteins, and others because of the easily commercialized synthesis [18][19][20].…”

Section: Introductionmentioning

confidence: 99%

A Novel Quantification Platform for Point-of-care testing of Circulating MicroRNAs Based on Allosteric Spherical Nanoprobe

Tian

Yuan

Liu

et al. 2020

Preprint

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MiRNA-150, a gene regulator that has been revealed to be abnormal expression in non-small cell lung cancer ( NSCLC ), can be regarded as a serum indicator for diagnosis and monitoring of NSCLC . Herein, a new sort of nanoprobe, termed allosteric spherical nanoprobe, was first developed to sense miRNA-150. Compared with conventional hairpin, this new nanoprobe possesses more enrichment capacity and reaction cross section. Structurally, it consists of magnetic nanoparticles and dual-hairpin. In the absence of miRNA-150, the spherical nanoprobes form hairpin structure through DNA self-assembly, which could promote the Förster resonance energy transfer (FRET) of fluorophore (FAM) and quencher (BHQ1) nearby. However, in the presence of target, the target-probe hybridization can open the hairpin and form the active “Y” structure which separated fluorophore and quencher to yield a “signal on” fluorescence. In the manner of multipoint fluorescence detection , the target-bound allosteric spherical nanoprobe could provide a high detection sensitivity with a linear range of 100 fM to 10 nM and a detection limit of 38 fM. More importantly, the proposed method could distinguish the expression of serum miRNA-150 among NSCLC patients and healthy people. Finally, we hoped that the potential bioanalytical application of this nanoprobe strategy will pave the way for point-of-care testing (POCT).

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Section: Introductionmentioning

confidence: 99%

A Novel Quantification Platform for Point-of-care testing of Circulating MicroRNAs Based on Allosteric Spherical Nanoprobe

Tian

Yuan

Liu

et al. 2020

Preprint

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“…38 For molecular fluorophores, it often derives from the formation of nonluminescent associates. 43 Although well-passivated semiconductor NPs are not likely to form such complexes due to the insulating property of their organic capping ligands, quenching by aggregation is often be observed. 44 The possible explanation for the quenching of semiconductor NPs in highly concentrated state is the energy transfer to the nonluminescent NPs, where nonradiative dots behave as the sinks of energy, as if they are defect levels in crystals, leading to a significant decrease in overall PL QY.…”

Section: Introductionmentioning

confidence: 99%

Improvement of Optical Properties for Semiconductor Nanoparticles by the Precise Control of Electron and Energy Transfer

Uematsu

2017

Electrochemistry

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This paper reviews my recent investigations on fluorescent semiconductor nanoparticles based on the observation of photoluminescence quenching caused by photoinduced electron transfer to surrounding molecules. Semiconductor nanoparticles having photoluminescence at room temperature were quenched intentionally by the addition of redox species. The magnitude of quenching was greatly varied according to the interaction between the nanoparticles and quenchers, redox potential of quenchers, and the states of surface ligands protecting the semiconductor nanoparticles. The study began from the basic investigations on factors determining the magnitude of quenching and several material sensing systems were proposed as output of research. The phenomenon was then used to investigate the condition of surface ligands by using the strong distance dependence of the photoinduced electron transfer efficiency. Quantitative analyses of quenching revealed the differences in protection ability between the types of ligands. In the last part of this paper, I mention the possibility of semiconductor nanoparticles as practical fluorescence materials. The precise control of the distance between the nanoparticles realized emission from condensed nanoparticles with photoluminescence quantum yield over 80%.

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“…However, alternative approaches are intensely being pursued to achieve free-space collection and flexibility in addressing single emitters. As a matter of fact, these conditions are paradigmatic for the development of single-photon sources in quantum optics 7,8 , fluorescence-based microscopy 9 and spectroscopy 10 , as well as for sensing applications 11 . Scanning optical microcavities, for example, provide enhanced light-matter interaction but suffer from a limited spectral bandwidth 12,13,14 .…”

Section: Introductionmentioning

confidence: 99%

Beaming light from a quantum emitter with a planar optical antenna

Checcucci

Lombardi

Rizvi³

et al. 2016

Light Sci Appl

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The efficient interaction of light with quantum emitters is crucial to most applications in nano and quantum photonics, such as sensing or quantum information processing. Effective excitation and photon extraction are particularly important for the weak signals emitted by a single atom or molecule. Recent works have introduced novel collection strategies, which demonstrate that large efficiencies can be achieved by either planar dielectric antennas combined with high numerical aperture objectives or optical nanostructures that beam emission into a narrow angular distribution. However, the first approach requires the use of elaborate collection optics, while the latter is based on accurate positioning of the quantum emitter near complex nanoscale architectures; hence, sophisticated fabrication and experimental capabilities are needed. Here we present a theoretical and experimental demonstration of a planar optical antenna that beams light emitted by a single molecule, which results in increased collection efficiency at small angles without stringent requirements on the emitter position. The proposed device exhibits broadband performance and is spectrally scalable, and it is simple to fabricate and therefore applies to a wide range of quantum emitters. Our design finds immediate application in spectroscopy, quantum optics and sensing.

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Introduction to Special Issue on “Fluorescence-Based Sensing Technologies”

Abstract: The application of fluorescence-based technologies to sensing applications in biosciences and related industries is growing. [...]

Cited by 5 publications

References 5 publications

A Novel Quantification Platform for Point-of-care testing of Circulating MicroRNAs Based on Allosteric Spherical Nanoprobe

A Novel Quantification Platform for Point-of-care testing of Circulating MicroRNAs Based on Allosteric Spherical Nanoprobe

Improvement of Optical Properties for Semiconductor Nanoparticles by the Precise Control of Electron and Energy Transfer

Beaming light from a quantum emitter with a planar optical antenna

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