Introgression and targeting of the Pl37 and Pl38 genes for downy mildew resistance from wild Helianthus annuus and H. praecox into cultivated sunflower (Helianthus annuus L.)

Qi, Lili; Talukder, Zahirul I.; J, G; Seiler, Gerald J.

doi:10.1007/s00122-023-04316-y

Cited by 4 publications

(4 citation statements)

References 58 publications

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“…The wild H. annuus accession (PI 435417) originally collected in Texas in 1976 was identified to be resistant to new P. halstedii races in 2005 (Gulya, 2005), with the DM R gene from PI 435417, named Pl 37 , and recently mapped to sunflower chromosome 4. Another new DM R gene, named Pl 38 , derived from the H. praecox accession PRA-417, was recently mapped to sunflower chromosome 2 (Qi et al, 2023). Here, we report the introgression of the new DM R genes, Pl ) is an improved oilseed derivative of Rf PRA-417 (PI 596749).…”

Section: Introductionmentioning

confidence: 81%

“…Pl 1 and Pl 2 were incorporated into all widely grown hybrids and cultivars in Europe and North America, remaining fairly stable for about 15 years, until the discovery of the new P. halstedii races in 1987 (Gulya et al, 1991;Miller & Gulya, 1987Vrânceanu & Stoenescu, 1970;Zimmer & Kinman, 1972). Since then, more than 30 new resistance genes to the DM pathogen have been discovered in sunflower and its wild species (for review, see Qi et al [2023]). Meanwhile, the P. halstedii pathogen co-evolved with the use of DM resistance genes in large-scale production, with a total of 44 P. halstedii races reported globally in 2017 (Trojanová et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

“…Wild sunflower species are a valuable source for DM R genes (Seiler et al, 2017). Of the 31 Pl genes previously mapped to the sunflower genome, ∼80% are derived from wild sunflower species, including the wild annual species H. annuus, H. argophyllus, and H. praecox and the wild perennial species H. tuberosus, H. tomentosus, and H. resinosus (Qi et al, 2023). The wild H. annuus accession (PI 435417) originally collected in Texas in 1976 was identified to be resistant to new P. halstedii races in 2005 (Gulya, 2005), with the DM R gene from PI 435417, named Pl 37 , and recently mapped to sunflower chromosome 4.…”

Section: Introductionmentioning

confidence: 99%

“…HA-DM16 (Pl 38 Pl 38 ) is an improved oilseed derivative of Rf PRA-417 (PI 596749) with the new DM R gene Pl 38 originally derived from sunflower wild H. praecox accession PRA-417 and genetically mapped to sunflower chromosome 2(Qi et al, 2023). Three BC 1 F 3 families selected from 58 Rf PRA-417T A B L E 1Note: Data fromQi et al (2023). Abbreviations: R, resistant; S, susceptible.…”

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confidence: 99%

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Registration of HA‐DM15 and HA‐DM16 oilseed sunflower germplasms with resistance to sunflower downy mildew

Qi,

Seiler

2023

J of Plant Registrations

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Sunflower (Helianthus L.) production ranked third in global oilseed production in 2021. Downy mildew (DM), caused by the oomycete pathogen Plasmopara halstedii (Farl.) Berl. et de Toni, is a major constraint for worldwide sunflower production, causing significant yield losses in susceptible hybrids. The search for new sources of resistance to DM is the primary objective of all sunflower breeding programs. Germplasms HA‐DM15 (Reg. no. GP‐385, PI 702359) and HA‐DM16 (Reg. no. GP‐386, PI 702360) were developed by introgressing two new DM resistance genes, Pl37 and Pl38, from the wild sunflower species H. annuus and H. praecox into oilseed sunflower, respectively. The BC1F3‐derived HA‐DM15 harboring the Pl37 gene was selected from the cross of HA 89//NMS HA 89/wild H. annuus PI 435417. The germplasm line HA‐DM16 carrying the Pl38 gene is an improved oilseed derivative of Rf PRA‐417 with DM resistance originally derived from the wild H. praecox PRA‐417 from the cross of cytoplasmic male sterile (CMS) HA89*2/wild H. praecox PRA‐417. HA‐DM15/Pl37 and HA‐DM16/Pl38 are immue to the most predominant and virulent DM races idenfied in North America and Europe. Pl37 and Pl38 have been genetically mapped to sunflower chromosomes 4 and 2, respectively, with single nucleotide polymorphism markers linked to the both genes facilitating their transfer to elite sunflower lines by marker‐assisted selection, providing new and diverse sources for sunflower DM‐resistance breeding.

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Section: Introductionmentioning

confidence: 81%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

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Registration of HA‐DM15 and HA‐DM16 oilseed sunflower germplasms with resistance to sunflower downy mildew

Qi,

Seiler

2023

J of Plant Registrations

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Development and characterization of a new sunflower source of resistance to race G of Orobanche cumana Wallr. derived from Helianthus anomalus

Fernández-Melero,

del Moral,

Todesco

et al. 2024

Theor Appl Genet

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Key message A new OrAnom1 gene introgressed in cultivated sunflower from wild Helianthus anomalusconfers late post-attachment resistance toOrobanche cumana race G and maps to a target interval in Chromosome 4 where two receptor-like kinases (RLKs) have been identified in theH. anomalusgenome as putative candidates. Abstract Sunflower broomrape is a parasitic weed that infects sunflower (Helianthus annuus L.) roots causing severe yield losses. Breeding for resistance is the most effective and sustainable control method. In this study, we report the identification, introgression, and genetic and physiological characterization of a new sunflower source of resistance to race G of broomrape developed from the wild annual sunflower H. anomalus (accession PI 468642). Crosses between PI 468642 and the susceptible line P21 were carried out, and the genetic study was conducted in BC1F1, BC1F2, and its derived BC1F3 populations. A BC1F5 germplasm named ANOM1 was developed through selection for race G resistance and resemblance to cultivated sunflower. The resistant trait showed monogenic and dominant inheritance. The gene, named OrAnom1, was mapped to Chromosome 4 within a 1.2 cM interval and co-segregated with 7 SNP markers. This interval corresponds to a 1.32 Mb region in the sunflower reference genome, housing a cluster of receptor-like kinase and receptor-like protein (RLK-RLP) genes. Notably, the analysis of the H. anomalus genome revealed the absence of RLPs in the OrAnom1 target region but featured two RLKs as possible OrAnom1 candidates. Rhizotron and histological studies showed that OrAnom1 determines a late post-attachment resistance mechanism. Broomrape can establish a vascular connection with the host, but parasite growth is stopped before tubercle development, showing phenolic compounds accumulation and tubercle necrosis. ANOM1 will contribute to broadening the genetic basis of broomrape resistance in the cultivated sunflower pool and to a better understanding of the molecular basis of the sunflower-broomrape interaction.

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A novel strategy to develop powdery mildew resistant phenotypes and diagnose reaction to Golovinomyces latisporus in sunflower (Helianthus annuus L.)

Mulpuri,

Baby Thomas,

Meena

et al. 2024

Physiological and Molecular Plant Pathology

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Introgression and targeting of the Pl37 and Pl38 genes for downy mildew resistance from wild Helianthus annuus and H. praecox into cultivated sunflower (Helianthus annuus L.)

Cited by 4 publications

References 58 publications

Registration of HA‐DM15 and HA‐DM16 oilseed sunflower germplasms with resistance to sunflower downy mildew

Registration of HA‐DM15 and HA‐DM16 oilseed sunflower germplasms with resistance to sunflower downy mildew

Development and characterization of a new sunflower source of resistance to race G of Orobanche cumana Wallr. derived from Helianthus anomalus

A novel strategy to develop powdery mildew resistant phenotypes and diagnose reaction to Golovinomyces latisporus in sunflower (Helianthus annuus L.)

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