Introgression mapping in the grasses

King, Julie; Armstead, Ian; Donnison, Iain S.; Harper, John A.; Roberts, Luned; Thomas, Howard; Ougham, Helen J.; Thomas, Ann; Huang, Lin; King, I. P.

doi:10.1007/s10577-006-1103-0

Cited by 20 publications

(17 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…4). LG3 has been intensively analyzed by introgression mapping and the presence of an NOR as noted previously [41], was also found in the present work. Thus Q-banding combined with FISH mapping has enabled LG3 to be identified as chromosome 2 and described by its size, banding pattern and morphology.…”

Section: Discussionsupporting

confidence: 78%

Fluorescence chromosome banding and FISH mapping in perennial ryegrass, Lolium perenne L.

et al. 2016

View full text Add to dashboard Cite

BackgroundThe unambiguous identification of individual chromosomes is a key part of the genomic characterization of any species. In this respect, the development and application of chromosome banding techniques has revolutionised mammalian and especially, human genomics. However, partly because of the traditional use of chromosome squash preparations, consistent fluorescence banding has rarely been achieved in plants. Here, successful fluorescence chromosome banding has been achieved for the first time in perennial ryegrass (Lolium perenne), a forage and turf grass with a large genome and a symmetrical karyotype with chromosomes that are difficult to distinguish.ResultsBased on flame-dried chromosome preparations instead of squashes, a simple fluorescence Q-banding technique using quinacrine mustard, unambiguously identified each chromosome and enabled the development of a banded karyotype and ideogram of the species. This Q-banding technique was also shown to be compatible with sequential FISH mapping enabling labelled genes and molecular markers to be precisely assigned to specific cytogenetic bands. A technique for DAPI-banding, which gave a similar pattern to Q-banding, was also introduced. This was compatible with FISH mapping and was used to anchor a single copy gene from an earlier mapped linkage group of L. perenne, thus providing a step towards integration of the genetic and cytogenetic maps.ConclusionsBy enabling the allocation of genes mapped by other methods to physically identified chromosome positions, this work will contribute to a better understanding of genomic structures and functions in grasses.

show abstract

Section: Discussionsupporting

confidence: 78%

Fluorescence chromosome banding and FISH mapping in perennial ryegrass, Lolium perenne L.

et al. 2016

View full text Add to dashboard Cite

show abstract

“…In Arabidopsis , most of the recombination events in hotspots were localized to non-coding regions [Drouaud et al, 2006;Mézard, 2006]. Similarly, King et al [2007] found that most of the functionally annotated genes were located in regions with low recombination rates in Lolium / Festuca introgression lines.…”

mentioning

confidence: 82%

Physical Distribution of Homoeologous Recombination in Individual Chromosomes of <i>Festuca pratensis </i>in <i>Lolium multiflorum</i>

Kopecký

Havránková

Loureiro

et al. 2010

Cytogenet Genome Res

View full text Add to dashboard Cite

Crossing over-based recombination is a powerful tool for generating new allelic combinations during sexual reproduction. It usually occurs between homologous chromosomes. However, under some conditions, homoeologues may also be capable of crossing over. Whether homologous and homoeologous crossovers are equivalent and governed by the same rules has never been established. Here we report on chromosome distribution of homoeologous crossovers in a unique system of Festuca × Lolium hybrids. Unlike in most other hybrids, in these intergeneric hybrids, homoeologous chromosomes are capable of pairing and crossing over with frequencies approaching that of homologues. At the same time, genome divergence makes cytological detection of chromosome recombination feasible. We analyzed the distribution of homoeologous recombination along individual chromosomes in a complete set of intergeneric single chromosome substitutions fromF. pratensisinto tetraploid L. multiflorum. Homoeologous recombination sites were not evenly distributed along the chromosomes, being concentrated in intercalary regions of the arms and reduced in proximal and distal regions. Several recombination hotspots and cold spots were found along individual chromosomes and the recombination was not affected by the presence of a secondary constriction. Our results indicate that despite the uneven distribution of homoeologous recombination, introgression of any part of the F. pratensis genome into L. multiflorum is feasible.

show abstract

“…Introgression mapping is a viable method for delineating the genetic differences between NILs (Nichols et al, 2006;King et al, 2007). Nichols et al (2006) showed that fine-mapping of NILs could identify introgressed regions in chromosomes.…”

mentioning

confidence: 99%

Identification of Candidate Genes Underlying an Iron Efficiency Quantitative Trait Locus in Soybean

et al. 2012

View full text Add to dashboard Cite

Prevalent on calcareous soils in the United States and abroad, iron deficiency is among the most common and severe nutritional stresses in plants. In soybean (Glycine max) commercial plantings, the identification and use of iron-efficient genotypes has proven to be the best form of managing this soil-related plant stress. Previous studies conducted in soybean identified a significant iron efficiency quantitative trait locus (QTL) explaining more than 70% of the phenotypic variation for the trait. In this research, we identified candidate genes underlying this QTL through molecular breeding, mapping, and transcriptome sequencing. Introgression mapping was performed using two related near-isogenic lines in which a region located on soybean chromosome 3 required for iron efficiency was identified. The region corresponds to the previously reported iron efficiency QTL. The location was further confirmed through QTL mapping conducted in this study. Transcriptome sequencing and quantitative real-time-polymerase chain reaction identified two genes encoding transcription factors within the region that were significantly induced in soybean roots under iron stress. The two induced transcription factors were identified as homologs of the subgroup lb basic helix-loop-helix (bHLH) genes that are known to regulate the strategy I response in Arabidopsis (Arabidopsis thaliana). Resequencing of these differentially expressed genes unveiled a significant deletion within a predicted dimerization domain. We hypothesize that this deletion disrupts the Fe-DEFICIENCY-INDUCED TRANSCRIPTION FACTOR (FIT)/bHLH heterodimer that has been shown to induce known iron acquisition genes.

show abstract

Introgression mapping in the grasses

Cited by 20 publications

References 36 publications

Fluorescence chromosome banding and FISH mapping in perennial ryegrass, Lolium perenne L.

Fluorescence chromosome banding and FISH mapping in perennial ryegrass, Lolium perenne L.

Physical Distribution of Homoeologous Recombination in Individual Chromosomes of <i>Festuca pratensis </i>in <i>Lolium multiflorum</i>

Identification of Candidate Genes Underlying an Iron Efficiency Quantitative Trait Locus in Soybean

Contact Info

Product

Resources

About