Introgression of clubroot resistance into an elite pak choi inbred line through marker‐assisted introgression breeding

Chen, Longzheng; Zhang, Xiaoqing; Xu, Hai; Song, Bo; Fan, Xiangtao

doi:10.1111/pbr.12379

Cited by 12 publications

(4 citation statements)

References 30 publications

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“…To compare with the previously identified clubroot disease loci/genes, we conducted PCR amplification of 29 markers associated with these loci/genes (CRa, CRc, CRk, CRd, Crr1, Crr2, Rcr1, and CrrA5) [ 17 , 19 , 21 , 24 , 51 , 52 , 53 , 54 ]. These markers had already been mapped to the B. rapa A-genome chromosomes.…”

Section: Methodsmentioning

confidence: 99%

Identification of Clubroot (Plasmodiophora brassicae) Resistance Loci in Chinese Cabbage (Brassica rapa ssp. pekinensis) with Recessive Character

Zhang,

Liu,

Zhou

et al. 2024

Genes

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The soil-borne pathogen Plasmodiophora brassicae is the causal agent of clubroot, a major disease in Chinese cabbage (Brassica rapa ssp. pekinensis). The host’s resistance genes often confer immunity to only specific pathotypes and may be rapidly overcome. Identification of novel clubroot resistance (CR) from germplasm sources is necessary. In this study, Bap246 was tested by being crossed with different highly susceptible B. rapa materials and showed recessive resistance to clubroot. An F2 population derived from Bap246 × Bac1344 was used to locate the resistance Quantitative Trait Loci (QTL) by Bulk Segregant Analysis Sequencing (BSA-Seq) and QTL mapping methods. Two QTL on chromosomes A01 (4.67–6.06 Mb) and A08 (10.42–11.43 Mb) were found and named Cr4Ba1.1 and Cr4Ba8.1, respectively. Fifteen and eleven SNP/InDel markers were used to narrow the target regions in the larger F2 population to 4.67–5.17 Mb (A01) and 10.70–10.84 Mb (A08), with 85 and 19 candidate genes, respectively. The phenotypic variation explained (PVE) of the two QTL were 30.97% and 8.65%, respectively. Combined with gene annotation, mutation site analysis, and real-time quantitative polymerase chain reaction (qRT-PCR) analysis, one candidate gene in A08 was identified, namely Bra020861. And an insertion and deletion (InDel) marker (co-segregated) named Crr1-196 was developed based on the gene sequence. Bra013275, Bra013299, Bra013336, Bra013339, Bra013341, and Bra013357 in A01 were the candidate genes that may confer clubroot resistance in Chinese cabbage. The resistance resource and the developed marker will be helpful in Brassica breeding programs.

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Section: Methodsmentioning

confidence: 99%

Identification of Clubroot (Plasmodiophora brassicae) Resistance Loci in Chinese Cabbage (Brassica rapa ssp. pekinensis) with Recessive Character

Zhang,

Liu,

Zhou

et al. 2024

Genes

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“…The hosts were screened for the presence of CR loci/genes ( CRa, CRc, CRk, CRd, Crr1, Crr2, Rcr1 , and CrrA5 ) using 29 linked markers ( Suwabe et al, 2003 , 2006 ; Hirai et al, 2004 ; Piao et al, 2004 ; Saito et al, 2006 ; Hayashida et al, 2008 ; Sakamoto et al, 2008 ; Matsumoto et al, 2012 ; Ueno et al, 2012 ; Chen et al, 2013 , 2016 ; Hatakeyama et al, 2013 ; Kato et al, 2013 ; Chu et al, 2014 ; Zhang et al, 2014 ; Pang et al, 2018 ). Total genomic DNA was isolated from the leaves using a cetyltrimethylammonium bromide (CTAB) method ( Saghai-Maroof et al, 1984 ) and subjected to polymerase chain reaction (PCR) analysis in a Veriti Thermal Cycler (Thermo Fisher Scientific).…”

Section: Methodsmentioning

confidence: 99%

Histopathology of the Plasmodiophora brassicae-Chinese Cabbage Interaction in Hosts Carrying Different Sources of Resistance

Liu

Strelkov²,

Sun³

et al. 2022

Front. Plant Sci.

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Clubroot is a serious soil-borne disease of crucifers caused by the obligate parasite Plasmodiophora brassicae. The genetic basis and histopathology of clubroot resistance in two Chinese cabbage (Brassica rapa ssp. pekinensis) inbred lines Bap055 and Bap246, challenged with pathotype 4 of P. brassicae, was evaluated. The Chinese cabbage cultivar “Juxin” served as a susceptible check. The resistance in Bap055 was found to be controlled by the CRa gene, while resistance in Bap246 fit a model of control by unknown recessive gene. Infection of the roots by P. brassicae was examined by inverted microscopy. Despite their resistance, primary and secondary infection were observed to occur in Bap055 and Bap246. Primary infection was detected at 2 days post-inoculation (DPI) in “Juxin,” at 4 DPI in Bap055, and at 6 DPI in Bap246. Infection occurred most quickly on “Juxin,” with 60% of the root hairs infected at 10 DPI, followed by Bap055 (31% of the root hairs infected at 12 DPI) and Bap246 (20% of the root hairs infected at 14 DPI). Secondary infection of “Juxin” was first observed at 8 DPI, while in Bap055 and Bap246, secondary infection was first observed at 10 DPI. At 14 DPI, the percentage of cortical infection in “Juxin,” Bap055 and Bap246 was 93.3, 20.0, and 11.1%, respectively. Although cortical infection was more widespread in Bap055 than in Bap246, secondary infection in both of these hosts was restricted relative to the susceptible check, and the vascular system remained intact. A large number of binucleate secondary plasmodia were observed in “Juxin” and the vascular system was disrupted at 16 DPI; in Bap055 and Bap246, only a few secondary plasmodia were visible, with no binucleate secondary plasmodia. The defense mechanisms and expression of resistance appears to differ between Chinese cabbage cultivars carrying different sources of resistance.

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“…- B. rapa (Inbred lines Chinese cabbage “CCR13685” (R) × Pak choi “GHQ11021” (S), using isolate with unknown race/pathotype): single dominant gene (Chen et al, 2016 )…”

Section: Sources Of R Genes For Key Pathogensmentioning

confidence: 99%

Current Status and Challenges in Identifying Disease Resistance Genes in Brassica napus

2017

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Brassica napus is an economically important crop across different continents including temperate and subtropical regions in Europe, Canada, South Asia, China and Australia. Its widespread cultivation also brings setbacks as it plays host to fungal, oomycete and chytrid pathogens that can lead to serious yield loss. For sustainable crop production, identification of resistance (R) genes in B. napus has become of critical importance. In this review, we discuss four key pathogens affecting Brassica crops: Clubroot (Plasmodiophora brassicae), Blackleg (Leptosphaeria maculans and L. biglobosa), Sclerotinia Stem Rot (Sclerotinia sclerotiorum), and Downy Mildew (Hyaloperonospora parasitica). We first review current studies covering prevalence of these pathogens on Brassica crops and highlight the R genes and QTL that have been identified from Brassica species against these pathogens. Insights into the relationships between the pathogen and its Brassica host, the unique host resistance mechanisms and how these affect resistance outcomes is also presented. We discuss challenges in identification and deployment of R genes in B. napus in relation to highly specific genetic interactions between host subpopulations and pathogen pathotypes and emphasize the need for common or shared techniques and research materials or tighter collaboration between researchers to reconcile the inconsistencies in the research outcomes. Using current genomics tools, we provide examples of how characterization and cloning of R genes in B. napus can be carried out more effectively. Lastly, we put forward strategies to breed resistant cultivars through introgressions supported by genomic approaches and suggest prospects that can be implemented in the future for a better, pathogen-resistant B. napus.

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Introgression of clubroot resistance into an elite pak choi inbred line through marker‐assisted introgression breeding

Cited by 12 publications

References 30 publications

Identification of Clubroot (Plasmodiophora brassicae) Resistance Loci in Chinese Cabbage (Brassica rapa ssp. pekinensis) with Recessive Character

Identification of Clubroot (Plasmodiophora brassicae) Resistance Loci in Chinese Cabbage (Brassica rapa ssp. pekinensis) with Recessive Character

Histopathology of the Plasmodiophora brassicae-Chinese Cabbage Interaction in Hosts Carrying Different Sources of Resistance

Current Status and Challenges in Identifying Disease Resistance Genes in Brassica napus

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