Introgression of genes for cotton leaf curl virus resistance and increased fiber strength from Gossypium stocksii into upland cotton (G. hirsutum)

Abstract

ABSTRACT. Cotton leaf curl virus disease is a major hurdle for successful cotton production in Pakistan. There has been considerable economic loss due to this disease during the last decade. It would be desirable to have cotton varieties resistant to this disease. We explored the possibility of transferring virus resistant genes from the wild species Gossypium stocksii into MNH-786, a cultivar of G. hirsutum. Hybridization was done under field condition at the Cotton Research Station, Multan, during 2010-11. Boll shedding was controlled by application of exogenous hormones. F 1 seeds were treated with 0.03% colchicine solution for 6 h and germinated. Cytological observations at peak squaring/flowering stage showed that these plants were hexaploid, having 2n = 6x = 78 chromosomes. The and size, bracteole area, bracteole dentation, flower size, pedicel size, and petal number and size. Moreover it possessed high fiber strength of 54.4 g/tex, which is 54% greater than that of the check variety, i.e. . The F 1 population did not show any symptom of CLCuVD in the field, tested by grafting with CLCuVD susceptible rootstock (var. S12). We conclude that it is possible to transfer CLCuVD resistance and high fiber strength from G. stocksii to G. hirsutum.

Evaluation of Cotton Leaf Curl Virus Resistance in BC1, BC2, and BC3 Progenies from an Interspecific Cross between Gossypium arboreum and Gossypium hirsutum

“…In general, wild diploid species of Gossypium possess resistance against many challenges, such as insects, pests, diseases, and many abiotic factors [37], [46]. Hence, there is a great need to exploit this resource to develop resistance against CLCuD in cultivated tetraploid species [2]. Cotton breeders have long tried to obtain hybrids between diploid and tetraploid species [47].…”

“…For example, Sacks and Robinson [41] transferred nematode ( Rotylenchulus reniformis ) resistance into tetraploid G. hirsutum. Chen et al [42] and Nazeer et al [2] employed Gossypium australe and Gossypium stocksii to introgress some novel genes for drought and CLCuD resistance into G. hirsutum, respectively. The interspecific hybridization is quite difficult, especially, between G. arboreum and G. hirsutum , and some scientists explored bridge lines for introgression of genes form wild species [43].…”

Genetic monitoring of introgressed alleles from Gossypium arboreum L. into G. hirsutum L. using SSR markers: a potential approach for bringing new alleles under cultivation

“…Sustainable cotton production is largely challenged by a number of biotic and abiotic factors (Rahman et al, 2012;Rahman, 2016). Among these, cotton leaf curl disease (CLCuD) is the most detrimental that suppress cotton production in Pakistan by approximately two million bales annually (Rajagopalan et al, 2012;Nazeer et al, 2014;Rahman et al, 2014). This disease has been spread to other major cotton growing countries like India and China (Cai et al, 2010: Singh et al, 2013.…”

“…Gossypium arboreum L. (till now) is immune and or highly resistant to all naturally occurring viral strains (including Burewala) causing leaf curl disease (Rahman et al, 2002(Rahman et al, , 2005Iqbal et al, 2015). Thus attempts were made to introgress alleles conferring resistance to the disease from G. arboreum into G. hirsutum using conventional means (Zhang et al, 2009;Maqbool et al, 2010;Nazeer et al, 2014;Rahman et al, 2014;Rahman, 2016). However, linkage drags of negatively linked traits ramined a major challenge in introgressing resistance in G. hirsutm varieties.…”

“…These concerns can be partly addressed using DNA markers for monitoring the introgression of alleles originating from the donor parent (G. arboreum) in backcross populations. In recent studies, alleles from Gossypium australe and Gossypium stocksii were introgressed in G. hirsutum (Chen et al, 2014;Nazeer et al, 2014). In the resultant progenies, fluctuations in expression of the desired traits (resistance to CLCuD) were noticed.…”