Introgression of ‘Ogura’ cytoplasm in cabbage alters its nutritional quality and antioxidant activities

Abstract

Ogura-based cytoplasmic male sterility (CMS) system is widely used for hybrid seed production in Brassica vegetables. The adverse effects associated with the introgression of Ogura cytoplasm on nutritional quality of cabbage are still unknown. Therefore, the effect of Ogura cytoplasm introgression on different quality traits in 17 lines of cabbage (Brassica oleracea var. capitata L.) was investigated. Experimental results revealed that introgression of Ogura cytoplasm altered different quality traits significantly. In general, the concentration of different nutritional compounds increased 3-5 times in some lines, while a 4-5-fold reduction was noticed in others. However, a drastic elevation in the concentration of cupric ion reducing antioxidant capacity (CUPRAC) and ferric reducing ability of plasma (FRAP) was observed in the CMS lines RRMA (29 times) and 5A (78 times), respectively. On the other hand, the amount of CUPRAC, anthocyanin and lycopene reduced severely in the CMS lines 9A (27 times), 1A (44 times) and CH2A (16 times), respectively. This alteration in different quality traits might be attributed to compatible / incompatible nucleo-cytoplasmic and mitochondrial gene interactions associated with the introgression of alien cytoplasm. Therefore, best performing CMS lines, viz. RRMA, RJA, PMA, 208A and 5A, with increased concentration of different nutritional compounds may be utilized in future breeding programs for quality improvement in cabbage. The positive correlations obtained between different quality traits offer the chances for selection of the genotypes with superior multiple quality traits. Further, outcomes of principal component analysis (PCA) and cluster analysis will aid in selection of suitable parental lines for quality F 1 hybrid development in cabbage.

Cabbage: Breeding and Genomics

“…The sources of high levels of nutritive and biologically active substances were found, mostly within central Russian and Dutch white cabbage accessions (Solovyova et al, 2014). Parkash et al (2019) studied the effect of 'Ogura' cytoplasm introgression on different quality traits in 17 lines of cabbage. In general, the concentration of different nutritional compounds increased 3-5 times in some lines, while a 4-5-fold reduction was noticed in others.…”

Accelerated Approaches for Cabbage Improvement

“…The present study aimed to investigate the impact of Ogura cytoplasm introgression on various quality traits in a total of 17 cabbage lines belonging to the species Brassica oleracea var. capitata L. [44]. The transfer of Ogura cytoplasm was accomplished by introducing cytoplasmic male sterility (CMS) from the EC-173419 source line of cabbage obtained from the National Bureau of Plant Genetic Resources in New Delhi, India.…”

Utilizing Ogura-CMS and doubled haploid lines of cabbage for new-generation hybrid breeding

“…To date, very meager information is available in the literature on genetic diversity analysis using CMS and DH lines of cabbage and their exploitation for uniform hybrid development in cabbage. Hence, at ICAR-IARI Regional Station, Katrain, we developed and maintained several cytoplasmic male sterile (Parkash et al 2019) and doubled haploid lines of cabbage (Bhatia et al 2018), out of which 26 promising lines were used for molecular diversity analysis and their further use in the development of next-generation hybrid of cabbage.…”