Introgression of the SbASR-1 Gene Cloned from a Halophyte Salicornia brachiata Enhances Salinity and Drought Endurance in Transgenic Groundnut (Arachis hypogaea) and Acts as a Transcription Factor

Tiwari, Vivekanand; Chaturvedi, Amit K.; Mishra, Avinash; Jha, Bhavanath

doi:10.1371/journal.pone.0131567

Cited by 83 publications

(71 citation statements)

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“…It has been reported that TaASR1 and SbASR1 induce the transcription of ROS scavenger-associated genes and reduce the accumulation of ROS (Hu et al, 2013; Tiwari et al, 2015). To analyze the role of SiASR4 in ROS scavenging in transgenic Arabidopsis , 3-week-old Col-0 and transgenic Arabidopsis grown in pots were not watered and were treated with salt for 6 days, respectively.…”

Section: Resultsmentioning

confidence: 99%

“…The loblolly pine ASR gene lp3 is induced by water deficit stress mediated by ABA (Padmanabhan et al, 1997). A Salicornia brachiata ASR gene, SbASR1 , enhances drought and high-salinity tolerance in transgenic groundnut (Tiwari et al, 2015). In foxtail millet, six ASR genes were identified.…”

Section: Introductionmentioning

confidence: 99%

“…In wheat, TaASR1 functioned as a positive factor in the regulation of stress-responsive and reactive oxygen species (ROS)-related gene expression in response to drought and osmotic stress (Hu et al, 2013). SbASR1 can reduce the accumulation of H 2 O 2 and

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radicals and induce the transcription of ROS scavenger-associated genes (Tiwari et al, 2015). The rice ASR gene OsASR5 binds to cis elements in the promoters of aluminum-responsive genes and regulates the expression of these genes (Arenhart et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

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SiASR4, the Target Gene of SiARDP from Setaria italica, Improves Abiotic Stress Adaption in Plants

Yang

et al. 2017

Front. Plant Sci.

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Drought and other types of abiotic stresses negatively affect plant growth and crop yields. The abscisic acid-, stress-, and ripening-induced (ASR) proteins play important roles in the protection of plants against abiotic stress. However, the regulatory pathway of the gene encoding this protein remains to be elucidated. In this study, the foxtail millet (Setaria italica) ASR gene, SiASR4, was cloned and characterized. SiASR4 localized to the cell nucleus, cytoplasm and cytomembrane, and the protein contained 102 amino acids, including an ABA/WDS (abscisic acid/water-deficit stress) domain, with a molecular mass of 11.5 kDa. The abundance of SiASR4 transcripts increased after treatment with ABA, NaCl, and PEG in foxtail millet seedlings. It has been reported that the S. italica ABA-responsive DRE-binding protein (SiARDP) binds to a DNA sequence with a CCGAC core and that there are five dehydration-responsive element (DRE) motifs within the SiASR4 promoter. Our analyses demonstrated that the SiARDP protein could bind to the SiASR4 promoter in vitro and in vivo. The expression of SiASR4 increased in SiARDP-overexpressing plants. SiASR4-transgenic Arabidopsis and SiASR4-overexpressing foxtail millet exhibited enhanced tolerance to drought and salt stress. Furthermore, the transcription of stress-responsive and reactive oxygen species (ROS) scavenger-associated genes was activated in SiASR4 transgenic plants. Together, these findings show that SiASR4 functions in the adaption to drought and salt stress and is regulated by SiARDP via an ABA-dependent pathway.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Section: Introductionmentioning

confidence: 99%

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SiASR4, the Target Gene of SiARDP from Setaria italica, Improves Abiotic Stress Adaption in Plants

Yang

et al. 2017

Front. Plant Sci.

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“…N-myristoylation facilitates protein folding and improves their thermostability (de-Jonge HR et al 2000). N-myristoylation was also found to be important for proper functioning of Arabidopsis gene, SOS3 ( Salt overly sensitive 3 ) having important role in plant salt tolerance (Ishitani et al 2000).…”

Section: Discussionmentioning

confidence: 99%

Development and Genetic Characterization of A Novel Herbicide (Imazethapyr) Tolerant Mutant in Rice (Oryza sativa L.)

Shoba

Raveendran

Manonmani

et al. 2017

Rice

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BackgroundIncreased water and labour scarcity in major rice growing areas warrants a shift towards direct seeded rice cultivation under which management of weeds is a major issue. Use of broad spectrum non-selective herbicides is an efficient means to manage weeds. Availability of rice genotypes with complete tolerance against broad-spectrum non-selective herbicides is a pre-requisite for advocating use of such herbicides. In the present study, we developed an EMS induced rice mutant, ‘HTM-N22‘, exhibiting tolerance to a broad spectrum herbicide, ‘Imazethapyr‘, and identified the mutations imparting tolerance to the herbicide.ResultsWe identified a stable and true breeding rice mutant, HTM-N22 (HTM), tolerant to herbicide, Imazethapyr, from an EMS-mutagenized population of approximately 100,000 M2 plants of an upland rice variety, Nagina 22 (N22). Analysis of inheritance of herbicide tolerance in a cross between Pusa 1656-10-61/HTM showed that this trait is governed by a single dominant gene. To identify the causal gene for Imazethapyr tolerance, bulked segregant analysis (BSA) was followed using microsatellite markers flanking the three putative candidate genes viz., an Acetolactate Synthase (ALS) on chromosome 6 and two Acetohydroxy Acid Synthase (AHAS) genes, one on chromosomes 2 and another on chromosome 4. RM 6844 on chromosome 2 located 0.16 Mbp upstream of AHAS (LOC_Os02g30630) was found to co-segregate with herbicide tolerance. Cloning and sequencing of AHAS (LOC_Os02g30630) from the wild type, N22 and the mutant HTM and their comparison with reference Nipponbare sequence revealed several Single Nucleotide Polymorphisms (SNPs) in the mutant, of which eight resulted in non-synonymous mutations. Three of the eight amino acid substitutions were identical to Nipponbare and hence were not considered as causal changes. Of the five putative candidate SNPs, four were novel (at positions 30, 50, 81 and 152) while the remaining one, S627D was a previously reported mutant, known to result in Imidazolinone tolerance in rice. Of the novel ones, G152E was found to alter the hydrophobicty and abolish an N myristoylation site in the HTM compared to the WT, from reference based modeling and motif prediction studies.ConclusionsA novel mutant tolerant to the herbicide “Imazethapyr” was developed and characterized for genetic, sequence and protein level variations. This is a HTM in rice without any IPR (Intellectual Property Rights) infringements and hence can be used in rice breeding as a novel genetic stock by the public funded organizations in the country and elsewhere.Electronic supplementary materialThe online version of this article (doi:10.1186/s12284-017-0151-8) contains supplementary material, which is available to authorized users.

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“…A bacterial mtlD gene encoding mannitol 1-phosphate dehydrogenase when expressed in groundnut cultivar GG20 under a constitutive promoter CaMV35s conferred drought tolerance through accumulation of mannitol (Bhauso et al, 2014). The genes from Salicornia brachiata, ascorbate peroxidase ( SbAPX ), and abscisic acid stress ripening ( SbASR ) genes when expressed in groundnut displayed salt and drought stress tolerance coupled with more chlorophyll retention and enhanced levels of relative water content under normal/stress conditions (Singh et al, 2014; Tiwari et al, 2015). The transcription factors from Arabidopsis, AtNAC2 (Patil et al, 2014), AtDREB1A (Sarkar et al, 2014, 2016) and horsegram, MuNAC4 (Pandurangaiah et al, 2014) were exhibited drought and salt stress tolerance in groundnut by reducing the membrane damage and improving scavenging of reactive oxygen species scavenging (ROS).…”

Section: Introductionmentioning

confidence: 99%

Expression of Pennisetum glaucum Eukaryotic Translational Initiation Factor 4A (PgeIF4A) Confers Improved Drought, Salinity, and Oxidative Stress Tolerance in Groundnut

et al. 2017

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Eukaryotic translational initiation factor 4A belong to family of helicases, involved in multifunctional activities during stress and non-stress conditions. The eIF4A gene was isolated and cloned from semi-arid cereal crop of Pennisetum glaucum. In present study, the PgeIF4A gene was expressed under the regulation of stress inducible Arabidopsis rd29A promoter in groundnut (cv JL-24) with bar as a selectable marker. The de-embryonated cotyledons were infected with Agrobacterium tumefaciens (LBA4404) carrying rd29A:PgeIF4A construct and generated high frequency of multiple shoots in phosphinothricin medium. Twenty- four T0 plants showed integration of both nos-bar and rd29A-PgeIF4A gene cassettes in genome with expected amplification products of 429 and 654 bps, respectively. Transgene copy number integration was observed in five T0 transgenic plants through Southern blot analysis. Predicted Mendelian ratio of segregation (3:1) was noted in transgenic plants at T1 generation. The T2 homozygous lines (L1-5, L8-2, and L16-2) expressing PgeIF4A gene were exhibited superior growth performance with respect to phenotypic parameters like shoot length, tap root length, and lateral root formation under simulated drought and salinity stresses compared to the wild type. In addition, the chlorophyll retention was found to be higher in these plants compared to the control plants. The quantitative real time—PCR results confirmed higher expression of PgeIF4A gene in L1-5, L8-3, and L16-2 plants imposed with drought/salt stress. Further, the salt stress tolerance was associated with increase in oxidative stress markers, such as superoxide dismutase accumulation, reactive oxygen species scavenging, and membrane stability in transgenic plants. Taken together our results confirmed that the PgeIF4A gene expressing transgenic groundnut plants exhibited better adaptation to stress conditions.

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Introgression of the SbASR-1 Gene Cloned from a Halophyte Salicornia brachiata Enhances Salinity and Drought Endurance in Transgenic Groundnut (Arachis hypogaea) and Acts as a Transcription Factor

Cited by 83 publications

References 78 publications

SiASR4, the Target Gene of SiARDP from Setaria italica, Improves Abiotic Stress Adaption in Plants

SiASR4, the Target Gene of SiARDP from Setaria italica, Improves Abiotic Stress Adaption in Plants

Development and Genetic Characterization of A Novel Herbicide (Imazethapyr) Tolerant Mutant in Rice (Oryza sativa L.)

Expression of Pennisetum glaucum Eukaryotic Translational Initiation Factor 4A (PgeIF4A) Confers Improved Drought, Salinity, and Oxidative Stress Tolerance in Groundnut

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