Intron 4 polymorphism of the endothelial nitric oxide synthase gene is associated with elevated blood pressure in type 2 diabetic patients with coronary heart disease

Pulkkinen, Arto; Viitanen, Laura; Kareinen, Anu; Lehto, Seppo; Laakso, Markku

doi:10.1016/s0021-9150(00)80941-7

Cited by 10 publications

(11 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…When statistical calculations were performed on the basis of the number of diseased vessels (0 vs. 1, 0 vs. 2 or 0 vs. 3 vessel disease), men carrying the a-allele (ba+aa) tended to have single-vessel coronary stenosis of over 50% less often than those who were homozygous for the b-allele. However, in men with two-or three-vessel disease, differences in stenosis were not observed, which is in line with previous findings of a Finnish case-control study by angiography [11]. Thus, our results are mainly in agreement with previous reports [9,10,11,21,22,23] that eNOS genotype does not associate significantly with the severity of CAD.…”

Section: Discussionsupporting

confidence: 93%

“…Although the results from clinical case-control studies have been far from consistent, the rare a-allele has previously been linked with no difference or an increased risk for MI or CAD [8,9,10,11,12,13,21,22,23]. Possible explanations for the discrepancies between our study and the previous clinical studies may be based on differences in distribution of eNOS genotypes in populations, due to selection factors and ethnicity and also on the fact that CAD risk factors were differently taken into account as confounding variables in the analyses.…”

Section: Discussionmentioning

confidence: 99%

“…The rare genotype of this polymorphism is aa, which occurs in 1%-10.6% of individuals from ethnically different populations. The aa genotype was first reported to be related to the severity of CAD in an Australian study [8], but this association was not confirmed in subsequent studies from populations in Japan [9] or Europe [10,11]. On the other hand, the eNOS 4aa genotype was also reported to be an independent risk factor for MI in Australia [8] and in Japan, especially among subjects lacking other conventional risk factors [12].…”

Section: O R I G I N a L A Rt I C L E Tarja A Kunnas · Erkki Ilveskomentioning

confidence: 96%

“…The eNOS gene harbours at least 11 polymorphic sites [6,7]. Because genetic variation could alter the expression and activity of eNOS, and therefore also contribute to the development of coronary artery disease (CAD) and myocardial infarction (MI), several genetic association studies have been conducted using numerous clinical patient populations [6,7,8,9,10,11,12,13,14,15].…”

Section: O R I G I N a L A Rt I C L E Tarja A Kunnas · Erkki Ilveskomentioning

confidence: 99%

See 3 more Smart Citations

Association of the endothelial nitric oxide synthase gene polymorphism with risk of coronary artery disease and myocardial infarction in middle-aged men

et al. 2002

View full text Add to dashboard Cite

Nitric oxide (NO), formed by endothelial constitutive nitric oxide synthase (eNOS) maintains endothelium-dependent vasodilatation and also mediates antithrombotic actions. The eNOS gene harbours a common polymorphism in intron 4 (4a/b), and some clinical studies have suggested an association of the rare a-allele with coronary artery disease (CAD) and myocardial infarction (MI). However, contradictory results have also been reported. We studied associations of eNOS polymorphism with CAD and MI in two prospective autopsy series comprising altogether 700 Caucasian Finnish men, who died suddenly. In ANCOVA, no significant differences in areas of atherosclerotic lesions and coronary stenosis percentages were found between men carrying the a-allele (ba+aa) compared with those homozygous for the b-allele. Subjects with the a-allele had significantly lower risk of MI (odds ratio 0.44, 95% confidence interval 0.25-0.77, P=0.004) compared with those carrying the bb genotype. Men with the a-allele also tended to have coronary thrombosis less often (odds ratio 0.43, 95% confidence interval 0.18-1.01, P=0.055). The eNOS gene 4a/b polymorphism was not associated with the extent of coronary atherosclerosis, but the a-allele of the variant seems to protect to some degree against the development of MI.

show abstract

Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Section: O R I G I N a L A Rt I C L E Tarja A Kunnas · Erkki Ilveskomentioning

confidence: 96%

Section: O R I G I N a L A Rt I C L E Tarja A Kunnas · Erkki Ilveskomentioning

confidence: 99%

See 2 more Smart Citations

Association of the endothelial nitric oxide synthase gene polymorphism with risk of coronary artery disease and myocardial infarction in middle-aged men

et al. 2002

View full text Add to dashboard Cite

show abstract

“…A number of variable tandem repeats (VNTR) and dinucleotide repeats have been identified in the ecNOS gene. Among previously reported polymorphisms in the ecNOS gene [7,8], ecNOS intron 4 b/a VNTR gene polymorphism was found to be associated with the incidences of many vascular diseases such as ischemic stroke [9], essential hypertension in Japanese [10], hypertension in type 2 diabetic patients [11], and coronary heart disease [12]. We previously reported an association between this polymorphism and patients with Kawasaki disease [13].…”

Section: Introductionmentioning

confidence: 99%

Lack of association between endothelial constitutive nitric oxide synthase (ecNOS 4 b/a) gene polymorphism and rheumatic heart disease

et al. 2009

View full text Add to dashboard Cite

Our aim in this work was to explore any possible association between ecNOS 4 b/a polymorphism and rheumatic heart disease (RHD). In this study, leukocyte DNA was extracted from 139 patients with RHD and 79 healthy control children. After amplification by PCR, the products were separated by electrophoresis in 6% polyacrylamide gel and visualized after ethidium bromide staining with UV light. PCR resulted in a 420-bp fragment (ecNOS4b) when five 27-bp repeats were present in intron 4 of the ecNOS gene, a 393-bp product (ecNOS4a)when only four repeats were present, and 420-bp and 393-bp fragments in heterozygous individuals (ecNOS4b/a). ecNOS4b/a genotyping in the control group gave the following results: b/b genotype (77.2%), b/a genotype(21.5%) and a/a genotype (1.3%). The frequencies in RHD group were as follows: b/b genotype (67.6%), b/a genotype (31.7%), and a/a genotype (0.7%). The b allele/a allele ratio was (88%/12%) in the control group and (83.5%/16.5%) in the RHD group. We found no statistical difference in genotype or allele frequency between these groups. The present study is the first to study the association between ecNOS 4b/a gene polymorphism and RHD, and to find a lack of any association between them. Further investigations of this gene polymorphism alone and in combination with other genes should be encouraged.

show abstract

Three polymorphisms of the eNOS gene and plasma levels of metabolites of nitric oxide in depressed Japanese patients: a preliminary report

Ikenouchi-Sugita

Yoshimura

Kishi

et al. 2011

Human Psychopharmacology

View full text Add to dashboard Cite

show abstract

Intron 4 polymorphism of the endothelial nitric oxide synthase gene is associated with elevated blood pressure in type 2 diabetic patients with coronary heart disease

Cited by 10 publications

References 0 publications

Association of the endothelial nitric oxide synthase gene polymorphism with risk of coronary artery disease and myocardial infarction in middle-aged men

Association of the endothelial nitric oxide synthase gene polymorphism with risk of coronary artery disease and myocardial infarction in middle-aged men

Lack of association between endothelial constitutive nitric oxide synthase (ecNOS 4 b/a) gene polymorphism and rheumatic heart disease

Three polymorphisms of the eNOS gene and plasma levels of metabolites of nitric oxide in depressed Japanese patients: a preliminary report

Contact Info

Product

Resources

About