Intussusceptive Pillar Formation in Developing Porcine Glomeruli

Abstract

Background/Aims: Intussusceptive angiogenesis (IA) is a dynamic process which contributes to vascular expansion and remodeling. Intraluminal pillars have long been the distinctive structural indicator of IA. However, the mechanism of their formation has not been fully elucidated. Methods: Using light and electron microscopy, we studied intussusceptive vascular growth in the developing porcine metanephric kidney. Results: We observed intraluminal pillars formed by endothelial cells in the vasculature of developing glomeruli. Their diameter was < 2.5 µm, consistent with the diameter of nascent pillars. TEM revealed that the majority of these pillars consisted only of endothelium. However, a central core of extracellular matrix (ECM) covered by endothelium, reminiscent of a more mature intussusceptive pillar, was also found in the lumen of a glomerular capillary. Perivascular cells or pericytes were not involved in the pillar structure during these stages of formation. Conclusion: This study shows ECM presence in a mature intussusceptive pillar without any perivascular cell involvement in the structure. This leads to the hypothesis that ECM deposition precedes the participation of these cells in the formation of intraluminal pillars during IA in porcine metanephric glomerular capillaries.

Coalescent angiogenesis—evidence for a novel concept of vascular network maturation

“…Generation of new blood vessels by this mechanism has also been termed intussusceptive microvascular growth. In contrast, intussusceptive arborization and intussusceptive branching remodeling [15] involve the reorganization of an existing capillary bed via expansion or pruning of vessel branches to optimize branching geometry and hemodynamics in existent vascular trees [16][17][18].…”