InvA Gene Sequencing of Salmonella typhimurium Isolated from Egyptian Poultry

El-Sebay, Nadine A.; Shady, Hala M. Abu; El-Zeedy, Seham A.; Samy, A. A.

doi:10.3923/ajsr.2017.194.202

Cited by 17 publications

(15 citation statements)

References 10 publications

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“…A study to compare the conventional isolation methods vs molecular identification procedure to detect Salmonella in broiler chicken meat has revealed that prevalence of Salmonella in meat samples was 12% in molecular method whereas it was 22% in the conventional method (Ibrahim et al, 2014). Use of PCR by targeting the sequences of hns and invA genes for rapid detection of Salmonella has been proven earlier too (El-Sebay et al, 2017). As a unique sequence to this genus available in fragment of invA gene, it has been verified as a precise PCR target (Rahn et al, 1992).…”

Section: Isolation Identification Confirmation and Serotyping Of Samentioning

confidence: 99%

Isolation and Identification of Salmonella spp. from Broiler Chicken Meat in Sri Lanka and their Antibiotic Resistance

et al. 2020

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Purpose : Salmonella infections continue to be a global problem with millions of humans and animal cases occurring annually. Broiler chicken plays a significant role causing Salmonella infections in Sri Lanka. Consumption of food contaminated with antimicrobial resistant Salmonella aggravates the problem. This study isolated, identified, and serotype the Salmonella spp. from broiler chicken meat in Sri Lanka and examined their antimicrobial susceptibility to be used in establishment of control measures. Research Method : Isolation of Salmonella species from broiler chicken meat was done by conventional method of isolation followed by polymerase chain reaction (PCR) confirmation. All PCR confirmed isolates of Salmonella were serotype and then, isolates were tested for antibiotic susceptibility using disc diffusion assay followed by the detection of antibiotic resistance genes using PCR. Findings : Broiler chicken meat in Sri Lanka is contaminated with Salmonella spp. at the prevalence of 11.6% and 8.9% of them carried hns and invA specific genes. Isolates were serotyped as Salmonella Typhimurium (47.8%), Salmonella Enteritidis (26.1%) and non typable (26.1%). Three isolates were resistant to ampicillin. Intermediate resistance was shown to three antibiotics and all the isolates were sensitive to nine antibiotics. Majority (56.5%) of Salmonella were sensitive to all the tested antibiotics. Prevalence of resistant genes for tetracycline, sulfonamides and aminoglycosides were within 4%-26%. None of the isolates except one (4%) carried chloramphenicol resistance genes. Originality / Value : Steps must be taken to minimize contamination of broiler chicken meat with Salmonella spp in Sri Lanka. Although, there is a low prevalence of antibiotic resistant isolates, its mere presence in broiler chicken is a warning signal of possibility of emergence of multidrug resistant strains.

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Section: Isolation Identification Confirmation and Serotyping Of Samentioning

confidence: 99%

Isolation and Identification of Salmonella spp. from Broiler Chicken Meat in Sri Lanka and their Antibiotic Resistance

et al. 2020

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“…Gene invA with 284 pb, which is also specific for Salmonella spp., has been used (Malorny et al, 2003;El-Baz et al, 2017). Edwards et al (2002) and El-Sebay et al (2017) have shown that all of the SP1 loci, in which invA genes are contained, share almost the same identity of the whole genomes in different serotypes (varying between 97.7 and 98.6% similarities). Once the presence of Salmonella spp.…”

Section: Introductionmentioning

confidence: 99%

Isolation and identification of Salmonella spp. in raw milk from dairy herds in Colombia

Castañeda-Salazar

Pulido-Villamarín

Ángel-Rodríguez

et al. 2021

Braz. J. Vet. Res. Anim. Sci.

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Salmonellosis is a foodborne disease (FBD) that affects public health and can cause death in people. Many outbreaks of Salmonellosis have been reported due to the contamination of raw milk and dairy products with the pathogen. To determine the prevalence of Salmonella spp. in milk samples from four dairy herds in the Sabana of Bogotá in 2017, 112 milk samples were taken directly from the mammary gland during milking. All milk samples were cultured and tested to isolate and identify Salmonella spp. using microbiological and molecular methods. Salmonella spp. prevalence of milk samples was found to be 20.5% (n=23). The main Salmonella serovars isolated were S. Newport (60.87%), S. Typhimurium (17.4%), S. Virchow, S. Bredeney, and S. Anatum (4.3% each one of the serovars). However, it was not possible to determine the Salmonella serotype in two isolates. The prevalence of Salmonella spp. in milk has not been studied extensively in Colombia. The 20.5% in the prevalence might be due to fact that the sample was taken directly from the mammary gland allowing a better chance of isolation by avoiding the dilutional effect of mixed milk from different cows in the buckets. This also suggests that the infection of the udder could have occurred by hematogenous dissemination or by milking machine contamination. This study highlights the need to implement measures to prevent contamination and reduce the problem in the herds, which will result in milk and dairy products with high standards of innocuity and quality and decrease the risk of foodborne illness.

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“…detection as it contains sequences that are unique to the genus Salmonella . [34] Invasion A is a factor in the outer membrane of Salmonella spp. that is responsible for entering the host epithelial cells in the intestines thus initiating infection [34].…”

Section: Introductionmentioning

confidence: 99%

“…[34] Invasion A is a factor in the outer membrane of Salmonella spp. that is responsible for entering the host epithelial cells in the intestines thus initiating infection [34]. The inv locus in S. enterica serovar Typhimurium was characterized and it was reported that invA is essential in the display of virulence in the intestine [35].…”

Section: Introductionmentioning

confidence: 99%

Detection and Molecular Identification of Salmonella Virulence Genes in Livestock Production Systems in South Africa

2019

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Livestock are an important source of protein and food for humans, however opportunistic pathogens such as Salmonella spp. turn livestock into vehicles of foodborne diseases. This study investigated the prevalence of virulence genes in Salmonella spp. isolated from livestock production systems in two provinces of South Africa. During the period from May to August, 2018, a total of 361 faecal (189), oral (100), environmental (soil (36) and water (27)) and feed (9) samples were randomly collected from different animals (cattle, sheep, goats, pigs, ducks and chickens) that were housed in small-scale livestock production systems from Eastern Cape and KwaZulu-Natal Provinces in South Africa. Salmonella spp. were isolated and identified using microbiological and DNA molecular methods. Salmonella spp. were present in 29.0% of the samples of which 30.2% belonged to the Salmonella enterica species as confirmed by the positive amplification of the species specific iroB gene. Virulence genes that were screened from livestock-associated Salmonella were invA, iroB, spiC, pipD and int1. Statistically significant associations (p < 0.05) were established between the virulence genes, sampling location, animal host as well as the season when samples were collected. Furthermore, statistically significant (p < 0.05) positive correlations were observed between most of the virulence genes investigated. This is one of the recent studies to detect and investigate livestock-associated Salmonella spp. in South Africa. This study highlights the importance of continuous monitoring and surveillance for pathogenic salmonellae. It also demonstrated the detection and prevalence of virulent Salmonella spp. harbored by livestock in South Africa. This study demonstrated the potential risks of pathogenic Salmonella enterica to cause foodborne diseases and zoonotic infections from farm-to-fork continuum using the global one-health approach.

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InvA Gene Sequencing of Salmonella typhimurium Isolated from Egyptian Poultry

Cited by 17 publications

References 10 publications

Isolation and Identification of Salmonella spp. from Broiler Chicken Meat in Sri Lanka and their Antibiotic Resistance

Isolation and Identification of Salmonella spp. from Broiler Chicken Meat in Sri Lanka and their Antibiotic Resistance

Isolation and identification of Salmonella spp. in raw milk from dairy herds in Colombia

Detection and Molecular Identification of Salmonella Virulence Genes in Livestock Production Systems in South Africa

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