Invariant Vα14 Chain NKT Cells PromotePlasmodium bergheiCircumsporozoite Protein-Specific Gamma Interferon- and Tumor Necrosis Factor Alpha-Producing CD8⁺T Cells in the Liver after Poxvirus Vaccination of Mice

Activation and Function of iNKT and MAIT Cells

“…Treatment of mice with αGalCer 1 or 2 days prior to infection with Plasmodium berghei or P. yoelii sporozoites protected them from acute parasitemia (GonzalezAseguinolaza et al, 2000). αGalCer also was shown to enhance the CD8 + T cells response when administered with experimental malaria vaccine (Korten et al, 2005). An increase in the number of IFNγ secreting lymphocytes was observed upon αGalCer administration.…”

Development of a Novel CD4+ TCR Transgenic Line That Reveals a Dominant Role for CD8+ Dendritic Cells and CD40 Signaling in the Generation of Helper and CTL Responses to Blood-Stage Malaria

“…Another important role for CD4 + T cells is the provision of help resulting in the licensing of dendritic cells (DC) for the effective priming of CD8 + T cells. However, although CD4 + T cell help is essential for primary responses to certain pathogens, such as HSV (11,18), it is dispensable during infection with influenza A virus, lymphocytic choriomeningitis virus, or Listeria monocytogenes (14,(19)(20)(21). It is understood that in the latter cases, sufficient engagement of receptors for pathogen-associated molecular patterns on DC by material derived from the infectious agent (6,22), or cytokines secreted by innate cells upon recognition of the pathogen (23,24), bypasses the need for CD4 + T cell help.…”

“…Although the use of these parasites in conjunction with model T cell lines has aided the study of antimalarial CD4 + and CD8 + T cell responses (6,(11)(12)(13)(14)(15), wild-type (WT) parasites and transgenic T cells capable of recognizing authentic parasitederived Ags are preferred, as they more closely resemble endogenous responses to natural infections. With this in mind, we recently generated a murine TCR transgenic line of PbA-specific CD8 + T cells, termed PbT-I (8,16).…”

Glycosphingolipids Gb3 and iGb3

“…Einzelzellsuspensionen von Thymi und Milzen wurden durch enzymatischen Gewebsverdau mittels Collagenase IA und DNAseI vorbereitet. Die Präparation von Leukozyten aus der Leber erfolgte mit Hilfe eines Percoll-Gradienten [7]. PE-markierte CD1d-Tetramere (Proimmune, Oxford, UK) wurden mit αGalCer (Axxora, Lörrach, Deutschland) beladen [10].…”