Invasion and MMP expression profile in desmoid tumours

Denys, Hannelore; Wever, Olivier De; Nusgens, Betty; Kong, Yuan; Sciot, Raf; Le, Anh-Thy; Dam, Kim Van; Jadidizadeh, Ali; Tejpar, Sabine; Mareel, Marcus; Alman, Benjamin A.; Cassiman, Jean Jacques

doi:10.1038/sj.bjc.6601661

Cited by 43 publications

(41 citation statements)

References 22 publications

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“…metalloproteinase-3 (MMP3, also known as stromelysin-1), which has been reported overexpressed in colorectal tumors and desmoid tumors, 20,21 is highly upregulated in Gadd45a -/-cells both in microarray and real-time PCR for over a hundred folds. Basement membrane (BM), which plays an essential role in promoting the motility of tumor cells, is an important component of ECM and laminins are the major constituents of BM.…”

Section: Gadd45a Decreases Migration and Invasion Abilities Of Hepg2 mentioning

confidence: 99%

Gadd45a inhibits cell migration and invasion by altering the global RNA expression

Shan

Zhan

et al. 2012

Cancer Biology & Therapy

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Section: Gadd45a Decreases Migration and Invasion Abilities Of Hepg2 mentioning

confidence: 99%

Gadd45a inhibits cell migration and invasion by altering the global RNA expression

Shan

Zhan

et al. 2012

Cancer Biology & Therapy

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“…RNA was extracted using Trizol regents (Life Technologies, Inc.) and converted to cDNA using reverse transcriptase with a poly-T primer. PCR primer pairs that amplify a product that crosses introns were chosen from previous publications [16][17][18][19][20] for reduced glyceraldehyde-phosphate dehydrogenase (GAPDH), b-catenin, genes known to be upregualted in the proliferative phase of wound healing (a-smooth muscle actin and type three collagen), and the bcatenin target genes MMP-7 and FN. PCR was performed under semiquantitative conditions as previously reported.…”

Section: Rna Analysismentioning

confidence: 99%

“…Two genes that are upregulated in mesenchymal cells in response to b-catenin mediated tcf-dependent transcription are matrix metalloproteinase seven (MMP-7) and fibronectin (FN). [8][9][10] Both matrix metalloproteinases and fibronectin are known to be upregulated during wound healing, and are also expressed in hyperplastic wounds. [11][12][13] Fibroblasts present during the proliferative healing phase have a cytological appearance resembling immature fibroblasts with abundant rough endoplasmic reticulum.…”

mentioning

confidence: 99%

Prolonged β-catenin stabilization and tcf-dependent transcriptional activation in hyperplastic cutaneous wounds

Cheon

Poon

et al. 2005

Laboratory Investigation

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Mesenchymal cells that accumulate during the proliferative phase of wound healing and that are present in hyperplastic wounds share cytologic similarities with the cells from fibroproliferative lesions in which there is activation of b-catenin-mediated transcription. Re-excision wounds from a previous biopsy and samples from hyperplastic cutaneous wounds were studied along with normal tissues. During normal wound healing, there was an increase in b-catenin protein level, peaking 4 weeks following the insult and returning towards baseline level by 12 weeks. Hyperplastic wounds exhibited a prolonged duration of elevated b-catenin, lasting more than 2 years following the initial injury. The level of expression of genes known to be upregulated in the proliferative phase of wound healing (a-smooth muscle actin and type three collagen), correlated with b-catenin protein level. The phosphorylation level of glycogen synthase kinase-3-b, a kinase important for b-catenin protein destabilization, correlated with b-catenin protein level. b-Catenin was transcriptionally active in these wounds as demonstrated by the expression of the b-catenin target genes (MMP-7 and FN) and by activation of a tcfreporter in primary cell cultures. b-catenin stabilization increases cell proliferation and motility in fibroblasts in vitro, and likely has a similar function during its transient elevation in the proliferative phase of normal wound healing. In hyperplastic wounds, there is dysregulation of b-catenin, maintaining the mesenchymal cells in a prolonged proliferative state. As such, b-catenin likely plays a central role in mesenchymal cells during the healing process, and is an appealing therapeutic target for disorders of wound healing.

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Section: Introductionmentioning

confidence: 99%

“…MMPs are zinc-dependent endopeptidases, which collectively can degrade all constituents of the ECM. Based on their structure and substrate specificity, they can be divided into subgroups of collagenases, stromelysins, gelatinases, membrane-type MMPs and other MMPs [7] .Breast cancer is the major cause of malignancy-related deaths of women worldwide. In Thailand, breast cancer is the second most common cancer among women and its incidence is increasing [8] .…”

mentioning

confidence: 99%

Inhibition of MMP-3 activity and invasion of the MDA-MB-231 human invasive breast carcinoma cell line by bioflavonoids

et al. 2009

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IntroductionMetastatic invasion is the primary cause of patient mortality during breast cancer progression. For a transformed cell to metastasize to a distant site in the body, it must first lose adhesion, penetrate and invade the surrounding extracellular matrix (ECM), enter the vascular system, and adhere to distant organs [1] . The inhibition of invasion of cancer cells has been an important strategy in cancer treatment [2] . A crucial step in the invasive processes is the proteolytic degradation of the ECM and basal membranes [3] . Several studies have shown that among the enzymes responsible for ECM degradation, the matrix metalloproteinases (MMPs) appear to play a critical role [4][5][6] . MMPs are zinc-dependent endopeptidases, which collectively can degrade all constituents of the ECM. Based on their structure and substrate specificity, they can be divided into subgroups of collagenases, stromelysins, gelatinases, membrane-type MMPs and other MMPs [7] .Breast cancer is the major cause of malignancy-related deaths of women worldwide. In Thailand, breast cancer is the second most common cancer among women and its incidence is increasing [8] . Cancer invasion and metastasis are leading causes of morbidity and mortality in patients with breast cancer. Several studies have demonstrated that upregulation of MMP-1, -2, -3, -7, -9, -13, and -14 is associated with breast cancer cell invasion [9][10][11] .MMP-3, also designated stromelysin 1, is a member of the matrixin family, which plays a pivotal role in the degradation and remodeling of the ECM. MMP-3 degrades several components of the ECM, such as fi bronectin, laminin, collagen type IV [12][13][14] and proteoglycans, and is thought to play an important role in rheumatoid arthritis, osteoarthritis [15,16] , tumor cell invasion and metastasis [17] . Aim: Stromelysin 1 (matrix metalloproteinase 3; MMP-3) is an enzyme known to be involved in tumor invasion and metastasis. In this study, fl avonoids from vegetables and fruits, such as quercetin, kaempferol, genistein, genistin, and daidzein, were tested for their ability to modulate the secretion and activity of MMP-3 in the MDA-MB-231 breast cancer cell line. In addition, we investigated the in vitro effects of fl avonoids on MDA-MB-231 cell invasion. Methods: The toxic concentration range of fl avonoids was evaluated using the MTT assay. The ability of MDA-MB-231 cells to invade was evaluated using a modifi ed Boyden chamber system. The activity of MMP-3 was determined by casein zymography. The secretion of MMP-3 was evaluated using Western blotting, casein zymography and confi rmed by ELISA. Results: Some putative fl avonoids, ie, quercetin and kaempferol (fl avonols), signifi cantly inhibited the in vitro invasion of MDA-MB-231 cells in a concentration-dependent manner, with IC 50 values of 27 and 30 μmol/L, respectively. Quercetin and kaempferol also reduced MMP-3 activity in a dose-dependent manner, with IC 50 values in the range of 30 μmol/L and 45 μmol/L, respectively. None of the fl avonoids had a signif...

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Invasion and MMP expression profile in desmoid tumours

Cited by 43 publications

References 22 publications

Gadd45a inhibits cell migration and invasion by altering the global RNA expression

Gadd45a inhibits cell migration and invasion by altering the global RNA expression

Prolonged β-catenin stabilization and tcf-dependent transcriptional activation in hyperplastic cutaneous wounds

Inhibition of MMP-3 activity and invasion of the MDA-MB-231 human invasive breast carcinoma cell line by bioflavonoids

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