Invasion of a murine in vitro blood-brain barrier co-culture model by dengue virus serotypes 1 to 4

Idris, Fakhriedzwan; Muharram, Siti Hanna; Zaini, Zainun; Alonso, Sylvie; Diah, Suwarni

doi:10.1007/s00705-019-04175-3

Cited by 8 publications

(8 citation statements)

References 78 publications

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“…Increasing the permeability of the BBB is a common mechanism of damage used by numerous viruses ( 76 , 77 ). The IEB and the BBB are formed by epithelial and endothelial cells, respectively.…”

Section: Hypothesismentioning

confidence: 99%

Neurological Symptoms of COVID-19: The Zonulin Hypothesis

et al. 2021

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The irruption of SARS-CoV-2 during 2020 has been of pandemic proportions due to its rapid spread and virulence. COVID-19 patients experience respiratory, digestive and neurological symptoms. Distinctive symptom as anosmia, suggests a potential neurotropism of this virus. Amongst the several pathways of entry to the nervous system, we propose an alternative pathway from the infection of the gut, involving Toll-like receptor 4 (TLR4), zonulin, protease-activated receptor 2 (PAR2) and zonulin brain receptor. Possible use of zonulin antagonists could be investigated to attenuate neurological manifestations caused by SARS-CoV-19 infection.

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Section: Hypothesismentioning

confidence: 99%

Neurological Symptoms of COVID-19: The Zonulin Hypothesis

et al. 2021

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“…In addition, viral RNA were detected in the brain, but not detected in the liver. The breaching mechanism and hyperpermeability of blood–brain barrier by DENV were primarily due to direct consequences of viral infection of endothelial cells ( 45 ).…”

Section: Discussionmentioning

confidence: 99%

Tree Shrew as a New Animal Model for the Study of Dengue Virus

2021

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Dengue virus is a significant public health threat worldwide; however, the pathogenesis of dengue disease remains poorly understood due to lack of appropriate small animal models. Tree shrews are an emerging experimental animal model for the study of human diseases due to their resemblance of genetic characteristics to primate animals. Herein we report that dengue infection in tree shrews elicits resemble clinical symptoms as in humans. Dengue fever (△2°C> normal body temperature) developed in ~22% healthy Chinese tree shrews from 2 through 33 days after infection with a low dose (1 ∗ 104 PFU/animal) of dengue virus serotype 2 or 3 intravenously or subcutaneously. The dengue genomic RNA and neutralizing antibodies were detected in ~78% of animals at days 7 and 15 post infection respectively. The serum levels of liver enzymes including aspartate transaminase, alanine aminotransferase and alkaline phosphatase were elevated with peaks at day 7 after infection. Modest thrombocytopenia and a slight decrease in the white blood cell count were observed. Intriguingly, although viral RNA was barely detectable in the liver by 48 days after infection, it was still evident in the brain. The intra-brain bleeding lesions in the intravenous infection group were more severe than those in the subcutaneous infection group. Our data demonstrate that primary dengue virus infection in tree shrews causes resemble clinical disease as in humans and thus tree shrews may be a suitable model for the study of dengue disease pathogenesis.

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“…Rodent brains were processed for triple immunogold electron microscopy using ASE, revealing a subcellular relocalization from the synapse periphery to within the synaptic cleft of GABA-A receptors during mutanthuntingtin evoked neurodegeneration (Rosas-Arellano et al, 2018). ASE was also key to visualize arginase, a protein below the SIP detection limit, expressed in a subset of marmoset microglia (Rodriguez-Callejas et al, 2019) and it was also applied to a murine in vitro blood-brain barrier model in order to visualize the changes in tight junctions that explain how dengue virus compromises its integrity (Idris et al, 2019).…”

Section: Discussionmentioning

confidence: 99%

“…A prominent presence of astrocytes has been previously detected in the dentate gyrus-CA3 hippocampal region of rodents (Kim et al, 2018) (Idris et al, 2019). Immunocytofluorescence in MDCK monolayer cells using Claudin 2 and Claudin 4 antibodies has previously revealed their localization in both the plasma membrane and in subcellular membrane organelles, although the antibody signal is not always easy to detect, especially in the case of Claudin 4.…”

Section: Single Immunohistofluorescence and Triple Labeling Using A Cmentioning

confidence: 99%

A Low Cost Antibody Signal Enhancer Improves Immunolabeling in Cell Culture, Primate Brain and Human Cancer Biopsy

et al. 2020

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This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

show abstract

Invasion of a murine in vitro blood-brain barrier co-culture model by dengue virus serotypes 1 to 4

Cited by 8 publications

References 78 publications

Neurological Symptoms of COVID-19: The Zonulin Hypothesis

Neurological Symptoms of COVID-19: The Zonulin Hypothesis

Tree Shrew as a New Animal Model for the Study of Dengue Virus

A Low Cost Antibody Signal Enhancer Improves Immunolabeling in Cell Culture, Primate Brain and Human Cancer Biopsy

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