Invasive American crayfish Pacifastacus leniusculus (Decapoda: Astacidae) in the Morava River (Slovakia)

Petrusek, Adam; Petrusková, Tereza

doi:10.2478/s11756-007-0059-7

Cited by 7 publications

(6 citation statements)

References 13 publications

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“…Similarly, we detected Agent Level A2 in 1 out of 2 investigated signal crayfish individuals from Slovakia. Invasions of American crayfish species were only recently reported from that country (Janský & Kautman 2007, Petrusek & Petrusková 2007, and, although the presence of the crayfish plague pathogen could be suspected, our analysis is the first to support this assumption with molecular data.…”

Section: Discussionsupporting

confidence: 58%

“…Each sample represented 1 crayfish individual. The samples were originally obtained for Kozubíková et al (2006Kozubíková et al ( , 2008Kozubíková et al ( , 2009Kozubíková et al ( , 2010 and Petrusek & Petrusková (2007) (see Table 2 for a list of localities and the number of analysed individuals). DNA was isolated using the DNeasy tissue kit (Qiagen) from soft abdominal cuticle (in individuals smaller than 5 cm a part of an uropod or telson was also included) as described by Kozubíková et al (2009), and the isolates were stored for 1 to 5 yr in −20°C.…”

Section: Dna Isolatesmentioning

confidence: 99%

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Re-examination of the prevalence of Aphanomyces astaci in North American crayfish populations in Central Europe by TaqMan MGB real-time PCR

Kozubíková

Vrålstad²,

Filipová³

et al. 2011

Dis. Aquat. Org.

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We applied quantitative TaqMan minor groove binder real-time polymerase chain reaction (PCR) on DNA isolates from soft abdominal cuticle of 460 North American crayfish Orconectes limosus and Pacifastacus leniusculus, previously tested for Aphanomyces astaci presence by conventional semi-nested PCR. Both approaches target the internal transcribed spacers of the pathogen nuclear ribosomal DNA, but apply different specific sequence motifs and technologies. The real-time PCR approach seems to provide higher sensitivity; the number of crayfish that tested positive increased from 23 to 32%, and 10 additional crayfish populations were indicated as hosting the disease agent. However, the vast majority of newly recorded positives contained very low agent levels, from 5 to 50 PCR-forming units. An isolate producing a false positive result by the semi-nested PCR (apparently undescribed Aphanomyces related to A. astaci) remained negative using the real-time PCR. The present study shows that previous results based on the seminested PCR were not substantially influenced by false positives but might have suffered from some false negatives at low agent levels. Combining alternative methods may therefore provide more reliable conclusions on the pathogen's presence. Further, we found positive correlation between the prevalence of infection carriers in American crayfish populations and the average amounts of A. astaci DNA detected in infected local crayfish individuals.

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Section: Discussionsupporting

confidence: 58%

Section: Dna Isolatesmentioning

confidence: 99%

Re-examination of the prevalence of Aphanomyces astaci in North American crayfish populations in Central Europe by TaqMan MGB real-time PCR

Kozubíková

Vrålstad²,

Filipová³

et al. 2011

Dis. Aquat. Org.

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“…So far, however, the species apparently did not spread further, either upstream to the Czech Republic or inland into Slovakia. Although original analyses did not suggest the infection by A. astaci (Petrusek and Petrusková, 2007), subsequent use of a more sensitive molecular method detected presence of the pathogen in this population (Kozubíková et al, 2011).…”

Section: Figure 7 Distribution Of Pacifastacus Leniusculus (Signal Crmentioning

confidence: 80%

“…The presence of signal crayfish, originating likely from introduction by Austrian fishermen or invasion from waterbodies in Austria, was confirmed in the border stretch of the river Morava between Slovakia and Austria in August 2006 (Petrusek and Petrusková, 2007), and since then the species has been observed in various backwaters of this river in Slovakia (E. Stloukal, pers. comm.).…”

Section: Figure 7 Distribution Of Pacifastacus Leniusculus (Signal Crmentioning

confidence: 93%

Continental-wide distribution of crayfish species in Europe: update and maps

Kouba

Petrusek

Kozák

2014

Knowl. Managt. Aquatic Ecosyst.

276

312

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Key-words: crayfish distribution, indigenous species, invasive species, native range, mapsRecently published astacological studies substantially improved available data on distribution of crayfish in various European regions. At the same time, spread of invasive species has been recorded, additional nonindigenous species became established in various countries, and losses of populations of native species due to crayfish plague and other negative factors were observed. We overview recent advances in this knowledge, and provide updated colour maps of the distribution of all crayfish species present in Europe. These maps are originally based on the data from the Atlas of Crayfish in Europe published in 2006 as a result of the CRAYNET project, and were further updated from more recently published reports, grey literature, and especially thanks to contributions and feedback of over 70 specialists from 32 countries. Separate maps are available for all indigenous crayfish species in Europe as well as for three most widespread non-indigenous crayfish species. Additionally, two maps give locations of known findings of crayfish species introduced to Europe after 1980. These newly established alien species have so far restricted distributions; however, the frequency of recent reports suggests that findings of such species resulting from releases of aquarium pets will further increase. RÉSUMÉ Répartition à l'échelle continentale des espèces d'écrevisses en Europe : mises à jour et cartes Mots-clés :distribution des écrevisses, espèces indigènes, espèces envahissantes, Les études astacologiques récemment publiées ont sensiblement amélioré les données disponibles sur la distribution des écrevisses dans les différentes régions européennes. Dans le même temps, la propagation des espèces envahissantes a été enregistrée, des espèces non indigènes supplémentaires se sont établies dans différents pays, et des pertes de populations d'espèces indigènes en raison de la peste des écrevisses et d'autres facteurs négatifs ont été observées. Nous synthétisons les progrès récents dans la connaissance, et fournissons des cartes en couleur mises à jour de la distribution de toutes les espèces d'écre-visses présentes en Europe. Ces cartes sont à l'origine basées sur les données de l'Atlas des écrevisses en Europe publié en 2006 à la suite du projet CRAYNET, et ont ensuite été mises à jour à partir des études publiées plus récemment, de la

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“…comm. ) and Romania (Pârvulescu et al, 2009); P. leniusculus in Croatia (Maguire et al, 2008), Norway (Johnsen et al, 2007) and Slovakia (Petrusek and Petruskova, 2007); and P. clarkii in Austria (Strasser and Patzner, 2005). The presence of P. clarkii in Cyprus is also confirmed (Stephanou, 1987;Perdikaris, 2009, pers.…”

Section: > Old Nics and Their Continued Spreadmentioning

confidence: 99%

A review of the ever increasing threat to European crayfish from non-indigenous crayfish species

Holdich¹,

Reynolds

Souty‐Grosset

et al. 2009

Knowl. Managt. Aquatic Ecosyst.

390

467

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Invasive American crayfish Pacifastacus leniusculus (Decapoda: Astacidae) in the Morava River (Slovakia)

Cited by 7 publications

References 13 publications

Re-examination of the prevalence of Aphanomyces astaci in North American crayfish populations in Central Europe by TaqMan MGB real-time PCR

Re-examination of the prevalence of Aphanomyces astaci in North American crayfish populations in Central Europe by TaqMan MGB real-time PCR

Continental-wide distribution of crayfish species in Europe: update and maps

A review of the ever increasing threat to European crayfish from non-indigenous crayfish species

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