Invasive<i>Haemophilus influenzae</i>Disease, Europe, 1996–2006

Ladhani, Shamez; Slack, Mary; Heath, Paul T; Gottberg, Anne von; Chandra, Manosree; Ramsay, Mary

doi:10.3201/eid1603.090290

Cited by 194 publications

(152 citation statements)

References 36 publications

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“…Several recent surveys have utilized various study designs to monitor invasive H. influenzae disease in several countries throughout the world in the Hib conjugate vaccine era (1,3,10,19,20,22,23,27,35,46). The following statements summarize the main conclusions from these studies.…”

Section: Impact Of Hib Conjugate Vaccines On Invasive Non-hib Diseasementioning

confidence: 98%

Haemophilus influenzae Infections in the H. influenzae Type b Conjugate Vaccine Era

2011

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The widespread use of Haemophilus influenzae type b (Hib) conjugate vaccines has nearly eradicated invasive Hib disease where the vaccines are used. This success was accompanied by a shift in capsular serotypes of invasive H. influenzae disease, with nontypeable strains replacing type b strains as the most common bloodstream isolate, but there is no convincing evidence of a true increase in the incidence of non-serotype b invasive infections. H. influenzae causes predominantly mucosal infections. The introduction of vaccines for otitis media and global shifts in antimicrobial susceptibility emphasize the importance of continued surveillance of H. influenzae colonization and disease patterns.

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Section: Impact Of Hib Conjugate Vaccines On Invasive Non-hib Diseasementioning

confidence: 98%

Haemophilus influenzae Infections in the H. influenzae Type b Conjugate Vaccine Era

2011

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“…OMPs (1 mg) were incubated with 50 U benzonase (Sigma-Aldrich) and solubilized in 400 ml of freshly prepared rehydration buffer ( [4][5][6][7] [GE Healthcare Life Sciences], 1 mM phenylmethanesulfonylfluoride [USB, Affymetrix, Santa Clara, CA], and 4 mM (2-aminoethyl) benzenesulfonyl fluoride hydrochloride [Sigma-Aldrich]). Thereafter, overnight in-gel rehydration was done on a precast 7 cm (pH 4-7) immobilized pH gradient gel strip (Immobiline Drystrips; GE Healthcare Life Sciences).…”

Section: Extraction Of Omps and Two-dimensional Gel Electrophoresismentioning

confidence: 99%

“…In contrast, NTHi are often associated with acute otitis media, sinusitis and are responsible for exacerbations in patients suffering from chronic obstructive pulmonary disease or cystic fibrosis (2,5,6). After introduction of the Hib vaccine in the early 1990s, which has successfully abrogated Hib infections, the incidence of other non-Hib H. influenzae, in particular NTHi and serotype f (Hif), has steadily increased as reported in several countries of the Western world (7)(8)(9).…”

mentioning

confidence: 99%

Identification of a Haemophilus influenzae Factor H–Binding Lipoprotein Involved in Serum Resistance

Fleury

Hallström

et al. 2014

The Journal of Immunology

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Haemophilus influenzae is a Gram-negative human pathogen that resides in the upper respiratory tract. Encapsulated H. influenzae type b (Hib) and type f (Hif) are the most common serotypes associated with invasive disease. H. influenzae displays various strategies to circumvent the host innate immune response, including the bactericidal effect of the complement system. In this study, we identified an H. influenzae lipoprotein having the ability to bind factor H (FH), the major regulator of the alternative pathway of complement activation. This protein, named protein H (PH), was surface exposed and was found in all clinical Hib and Hif isolates tested. Deletion of the gene encoding for PH (lph) in Hib and Hif significantly reduced the interaction between bacteria and FH. When Hib and Hif PH variants were separately expressed in nontypeable (unencapsulated) H. influenzae, which did not bind FH, an increased FH affinity was observed. We recombinantly expressed the two PH variants in Escherichia coli, and despite sharing only 56% identical amino acids, both FH-binding Haemophilus proteins similarly interacted with the complement regulator FH short consensus repeats 7 and 18–20. Importantly, Hib and Hif resistance against the bactericidal effect of human serum was significantly reduced when bacterial mutants devoid of PH were tested. In conclusion, we have characterized a hitherto unknown bacterial protein that is crucial for mediating an interaction between the human pathogen H. influenzae and FH. This novel interaction is important for H. influenzae resistance against complement activation and will consequently promote bacterial pathogenesis.

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“…It is a major cause of bacterial meningitis in children aged under 5 years old and is also a significant agent of respiratory tract infections, including acute otitis media, sinusitis, pneumonia, and other serious invasive infections [1]. In elderly individuals, particularly those with underlying lung disease, Haemophilus influenzae can cause severe pneumonia.…”

Section: Introductionmentioning

confidence: 99%

Clinical Characteristics of Haemophilus influenzae at General Hospital in the Central Region of Japan

Minami¹,

Sakakibara²,

Imura³

et al. 2016

JBM

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Haemophilus influenzae is an important pathogen that caused several infection diseases, such as sinusitis, otitis media, sepsis, and meningitis. This study was conducted to find out the prevalence and antimicrobial susceptibility pattern of Haemophilus influenzae isolates at general hospital in the central region of Japan from December 2015 to January 2016. Haemophilus influenzae was identified by standard laboratory procedure. Antimicrobial susceptibility testing was performed by micro dilution assay according to CLSI recommendation. One hundred ninety-one Haemophilus influenzae were isolated, among which 95 (49.7%) were from male and 96 (50.3%) were from female. The age incidence of (0) years, (≤2) years, (≤5) years, and (6≤) years groups were 22(11.5%), 92(48.2%), 61(31.9%), and 16(8.4%), respectively. Positive samples were received mostly from the nasal discharge (177/92.7%), sputum (6/3.1%), tonsillar (6/3.1%), and pharynx (2/1.0%). Ceftriaxone was the most active antibiotics with 100% susceptible rates, followed by ciprofloxacin (99.5%) and minocycline (99%) in our study. Furthermore, we categorized four patterns: beta lactamase-negative ampicillin-sensitive strain (BLNAS), beta lactamase-negative ampicillin-resistant strain (BLNAR), beta lactamase-positive ampicillin resistant strain (BLPAR), and beta lactamase-positive amoxicillin-clavulanic acid-resistant strain (BLPACR) from those ampicillin susceptible results. The numbers of female were significant greater than those of male in BLPAR (p = 0.0336). With respect to antimicrobial susceptible pattern, there was no minocycline and piperacillin resistant strain in both BLNAS and BLNAR (p < 0.0001). Haemophilus influenzae infection spreads worldwide and inadequate use of antibiotics contributes to uptake of their new antimicrobial resistance. Continuous antimicrobial surveys are need for control the emergence and spread of antimicrobial resistance to reduce the morbidity and mortality.

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InvasiveHaemophilus influenzaeDisease, Europe, 1996–2006

Abstract: Incidence and case-fatality ratios are higher for non–type b than for type b infection.

Cited by 194 publications

References 36 publications

Haemophilus influenzae Infections in the H. influenzae Type b Conjugate Vaccine Era

Haemophilus influenzae Infections in the H. influenzae Type b Conjugate Vaccine Era

Identification of a Haemophilus influenzae Factor H–Binding Lipoprotein Involved in Serum Resistance

Clinical Characteristics of Haemophilus influenzae at General Hospital in the Central Region of Japan

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