Invasiveness of endometriotic cells in vitro

Gaetje, R.; Baumann, R; Herrmann, G.; Kotzian, Silvia; Starzinski‐Powitz, Anna

doi:10.1016/s0140-6736(95)92474-4

Cited by 151 publications

(76 citation statements)

References 6 publications

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“…As shown previously, primary peritoneal endometriotic cells 24,40 and endometriotic cell lines derived from peritoneal lesions (this article and Starzinski-Powitz et al 35 ) share features with carcinoma cells (absence of E-cadherin and invasive capacity) although they are not neoplastic. The question was whether additional parameters typical for invasive/metastatic tumor cells might be found in the endometriotic cell lines.…”

Section: Endometriotic Cell Lines Express N-cadherin An Invasion/migsupporting

confidence: 78%

“…24,40 It is conceivable that the invasion and metastasis of endometriotic cells resembles in some ways early micrometastasis of carcinomas. Here, it is postulated that a few CKϩ cells can reach distant locations such as lymph nodes and bone marrow because of early disruption of homotypic cell-cell contacts in the primary tumor.…”

Section: Discussionmentioning

confidence: 99%

“…24,38,39 One main characteristic of primary endometriotic cell cultures established from peritoneal biopsies is that they contain invasive cells, as previously shown in an in vitro collagen invasion assay. 24,40 To confirm this and to more easily characterize the invasive cells by immunostaining, we introduced the Matrigel invasion assay (Figure 1).…”

Section: In Vitro Growth and Invasive Properties Of Primary Endometrimentioning

confidence: 99%

See 2 more Smart Citations

Identification of an Invasive, N-Cadherin-Expressing Epithelial Cell Type in Endometriosis Using a New Cell Culture Model

Zeitvogel

Baumann

Starzinski‐Powitz

2001

The American Journal of Pathology

244

226

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Section: Endometriotic Cell Lines Express N-cadherin An Invasion/migsupporting

confidence: 78%

Section: Discussionmentioning

confidence: 99%

Section: In Vitro Growth and Invasive Properties Of Primary Endometrimentioning

confidence: 99%

See 1 more Smart Citation

Identification of an Invasive, N-Cadherin-Expressing Epithelial Cell Type in Endometriosis Using a New Cell Culture Model

Zeitvogel

Baumann

Starzinski‐Powitz

2001

The American Journal of Pathology

244

226

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“…MTT assay revealed that the cells transfected with the miR-29c mimic proliferated at a significantly lower rate, and transfection with miR-29c inhibitor had the opposite effect. Endometriotic cells have an invasive potential which may contribute to the pathogenesis of endometriosis (10). In the present study, we found that transfection with miR-29c mimic suppressed cell invasion ability, while transfection with the miR-29c inhibitor enhanced cell invasion ability.…”

Section: Discussionsupporting

confidence: 59%

“…Endometriosis displays a benign morphology; however, endometriotic cells have an invasive potential which is characterized by malignant cells (10). To date, to the best of our knowledge, there is no report available on the role of miR-29 in the endometrium.…”

Section: Introductionmentioning

confidence: 99%

miR-29c is downregulated in the ectopic endometrium and exerts its effects on endometrial cell proliferation, apoptosis and invasion by targeting c-Jun

Long

Wan

et al. 2015

International Journal of Molecular Medicine

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Abstract. Endometriosis is a prevalent and complex gynecological disease which affects 10% of women of reproductive age. Certain studies have suggested that a substantial number of microRNAs (miRNAs or miRs) are aberrantly or differentially expressed in the ectopic endometrium. To date, to the best of our knowlewdge, there is no report available on the role of miR-29 in the endometrium. In this study, we investigated the expression of the miR-29 family in the endometrium samples from women without endometriosis, as well as in paired ectopic and eutopic endometrium samples by reverse transcription-quantitative polymerase chain reaction (RT-qPCR). The results revealed that miR-29c was differentially expressed in the paired eutopic and ectopic endometrium samples. In addition, c-Jun was differentially expressed in the ectopic and eutopic endometrial tissues as determined by western blot analysis. Furthermore, the role of miR-29c in endometrial cell proliferation, invasion and apoptosis was examined in vitro. The results revealed that miR-29c exerted its effects on endometrial cells by suppressing cell proliferation and invasion, as well as promoting cell apoptosis. Furthermore, it was found that c-Jun was a novel target of miR-29c, and c-Jun reversed the effects of miR-29c on the proliferation, invasion and apoptosis of endometrial cells. To the best of our knowledge, this study is the first to identify miR-29c as a suppressor of endometriosis. Taken together, our results suggest that miR-29c exerts its effects on endometrial cell proliferation, apoptosis and invasion by inhibiting the expression of c-Jun. Our data may provide a novel potential therapeutic target for the treatment of endometriosis.

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Biology of Eutopic and Ectopic Endometrium in Women with Endometriosis

Klemmt

Starzinski‐Powitz

2011

Endometriosis

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Invasiveness of endometriotic cells in vitro

Cited by 151 publications

References 6 publications

Identification of an Invasive, N-Cadherin-Expressing Epithelial Cell Type in Endometriosis Using a New Cell Culture Model

Identification of an Invasive, N-Cadherin-Expressing Epithelial Cell Type in Endometriosis Using a New Cell Culture Model

miR-29c is downregulated in the ectopic endometrium and exerts its effects on endometrial cell proliferation, apoptosis and invasion by targeting c-Jun

Biology of Eutopic and Ectopic Endometrium in Women with Endometriosis

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