Invasiveness of transformed bladder epithelial cells

Abstract

The invasiveness of bladder tumors has been studied in man, experimental animals, and in tissue culture by numerous authors. The prognostic importance of cellular markers for invasiveness is stressed, and the usefulness of histopathological and cytologic grading, cytogenetic studies, antigenic investigations, and enzymatic characterization is discussed. The invasiveness of bladder cells has frequently been examined in transplantation and explantation experiments. In human urothelial cell cultures three grades of transformation are defined, and a correlation has been established between the invasiveness of these cell lines in a three-dimensional in vitro model and their tumorigenicity in nude mice. The mechanism of tumor invasion is discussed, and it is recommended in future research to make a distinction between invasion en bloc and cellular infiltration.

The spectrum of TP53 mutations in bladder carcinoma

“…(Human Tumor Cell Laboratory, Sloan-Kettering Cancer Center, personal communication), SCaBER (O'Toole et al, 1976), VM-CUB-2 (Williams, 1980), RT112 (O'Toole, unpublished), SW1710 (Kyriazis et al, 1984), HT1376 and HT1197 (Rasheed et al, 1977), S D (Paulie et al, 1983), 2535 (Elliot et al, 1974), SW800 (Kyriazis et al, 1984), IJM-UC-3 (Grossman et al, 1986), and EJ (Evans et al, 1977). The cell line 609 (Kieler, 1984), an immortal urothelial cell line which was established from the normal ureter of a patient with renal carcinoma, was also included.…”

Altered glycosylation of membrane glycoproteins in human uroepithelial cell lines

“…(1984) and by Kieler (1984) have made these cell lines a valuable tool in studies of various aspects of the biology of human transitional cell carcinoma (TCC) of the bladder (Bubenik et al, 1985;Christensen et al, 1984;DuS et al, 1985;Kieler, 1984;Vilien et al, 1983). The aim of the present study was to examine the expression of total cellular glycopeptides on these cells and to relate our findings to their growth properties in vitro, tumorigenic capacity in vivo and invasiveness in vitro.…”

Correlation between classification of human urothelial cell lines and HLA-A,B,C expression

“…Normal (TGr0) and slightly transformed (TGrI) cells differ from pre-malignant (TGrII) and malignant (TGrIII) cells in their morphology and finite life-span in culture. TGrIII cells differ from TGrII cells in their morphology [2], their tumourigenicity in nude mice [2,19], in their ability to invade normal tissue in vitro [2,10], in the glycosylation of membrane glycoproteins [1] and in their apparent lack of polymorphic HLA-A,B epitopes [18]. In the present study we demonstrate an inverse relationship between the TGrII and TGrIII classification of 9 human urothelial cell lines and their expression of the monomorphic part of HLA-A,B,C.…”