Inverse and Reversible Switching Gradient Surfaces from Mixed Polyelectrolyte Brushes

Ionov, Leonid; Houbenov, Nikolay; Sidorenko, Alexander; Stamm, Manfred; Luzinov, Igor; Minko, Sergiy

doi:10.1021/la048158a

Cited by 112 publications

(130 citation statements)

References 40 publications

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“…42 In the first step, a PBA layer with gradually changing thickness is prepared using annealing on the temperature-gradient stage ͑see Sec. II͒.…”

Section: Resultsmentioning

confidence: 99%

“…42,44,47 The grafted thickness of PBA gradually increased from about 1 to 7 nm from the left edge to right ͑Fig. 2͒.…”

Section: Methodsmentioning

confidence: 99%

“…40,41 These effects are at the heart of inverse and reversible switching of the gradient of water contact angle on the surface of mixed brushes with a gradually changing ratio between the components of the brushes. 42 Here, we report on the use of such mixed-gradient grafted polyelectrolyte brushes for stimuli-sensitive generation of surface gradients of proteins. The principle of this approach is illustrated in Fig.…”

Section: 27mentioning

confidence: 99%

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Stimuli-responsive command polymer surface for generation of protein gradients

et al. 2009

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Mixed polyelectrolyte brushes with a composition gradient were used as a platform for fabrication of stimuli-responsive command surfaces to control the generation of concentration gradients of adsorbed protein molecules. Switching between homogeneously adsorbed protein layers and adsorbed layers with protein concentration gradients was achieved by changing the pH of protein aqueous solutions. Protein adsorption and the direction of the adsorption gradient were tuned and also turned off and on or reversed by tuning the proton concentration in the pH range 4.0–8.6.

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“…42 In the first step, a PBA layer with gradually changing thickness is prepared using annealing on the temperature-gradient stage ͑see Sec. II͒.…”

Section: Resultsmentioning

confidence: 99%

“…42,44,47 The grafted thickness of PBA gradually increased from about 1 to 7 nm from the left edge to right ͑Fig. 2͒.…”

Section: Methodsmentioning

confidence: 99%

Section: 27mentioning

confidence: 99%

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Stimuli-responsive command polymer surface for generation of protein gradients

et al. 2009

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“…Switchable systems are designed to respond to an external signal that instigates a change in the material between disparate properties in order to control the behaviour of molecules in time. Examples of switchable systems include changes in surface wettability, charge and permeability [12][13][14] . Biodevices that are able to combine switchable and patterned properties to control biomolecules in both space and time enable advanced biomolecular manipulation and have enormous potential for the development of highly useful, effective and sophisticated biodevice systems.…”

Section: Introductionmentioning

confidence: 99%

A platform for the advanced spatial and temporal control of biomolecules

et al. 2006

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Manipulating biomolecules at solid/liquid interfaces is important for the development of various biodevices including microarrays. Smart materials that enable both spatial and temporal control of biomolecules by combining switchability with patterned surface chemistry offer unprecedented levels of control of biomolecule manipulation. Such a system has been developed for the microscale spatial control over both DNA and cell growth on highly doped p-type silicon. Surface modification, involving plasma polymerisation of allylamine and poly(ethlylene glycol) grafting with subsequent laser ablation, led to the production of a patterned surface with dual biomolecule adsorption and desorption properties. On patterned surfaces, preferential electro-stimulated adsorption of DNA to the allylamine plasma polymer surface and subsequent desorption by the application of a negative bias was observed. The ability of this surface to control both DNA and cell attachment in four dimensions has been demonstrated, exemplifying its capacity to be used for complex biological studies such as gene function analysis. This system has been successfully applied to living microarray applications and is an exciting platform for any system incorporating biomolecules.

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“…After the generation of a coverage gradient, the surface can be easily saturated with a differently functionalised copolymer, which allows the creation of gradients from different functional groups, such as peptides or biotin. The effect of temperature on the grafting rate was exploited by Ionov et al [71][72][73] They first coated a silica substrate with an anchoring layer of either silanes or a polymer and then a second layer of polymer. The degree of anchoring of the top-layer polymer, which was to form a brushlike coating, to the layer below is controlled by applying a temperature gradient.…”

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confidence: 99%

Surface-chemical and -morphological gradients

2008

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Surface gradients of chemistry or morphology represent powerful tools for the high-throughput investigation of interfacial phenomena in the areas of physics, chemistry, materials science and biology. A wide variety of methods for the fabrication of such gradients has been developed in recent years, relying on principles ranging from diffusion to time-dependent irradiation in order to achieve a gradual change of a particular parameter across a surface. In this review we have endeavoured to cover the principal fabrication approaches for surface-chemical and surface-morphological gradients that have been described in the literature, and to provide examples of their applications in a variety of different fields.

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Inverse and Reversible Switching Gradient Surfaces from Mixed Polyelectrolyte Brushes

Cited by 112 publications

References 40 publications

Stimuli-responsive command polymer surface for generation of protein gradients

Stimuli-responsive command polymer surface for generation of protein gradients

A platform for the advanced spatial and temporal control of biomolecules

Surface-chemical and -morphological gradients

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