Inverse Dose‐ and Time‐Dependent Effect of Basic Fibroblast Growth Factor on the Gene Expression of Collagen Type I and Matrix Metalloproteinase‐1 by Periodontal Ligament Cells in Culture

Palmon, Aaron; Roos, H.; Edel, J.; Zax, B.; Savion, Naphtali; Grosskop, A.; Pitaru, S.

doi:10.1902/jop.2000.71.6.974

Cited by 46 publications

(42 citation statements)

References 29 publications

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“…75,76 Flexor tendon tenocytes subjected to shear stress also demonstrated downregulation of type I collagen expression, 77 as did bone marrow stem cells seeded on a composite of tendon slices. 56 The presence of LDM significantly increased cell proliferation and matrix synthesis.…”

Section: Little Et Almentioning

confidence: 99%

Ligament-Derived Matrix Stimulates a Ligamentous Phenotype in Human Adipose-Derived Stem Cells

Little

Guilak

Ruch

2010

Tissue Engineering Part A

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Human adipose stem cells (hASCs) can differentiate into a variety of phenotypes. Native extracellular matrix (e.g., demineralized bone matrix or small intestinal submucosa) can influence the growth and differentiation of stem cells. The hypothesis of this study was that a novel ligament-derived matrix (LDM) would enhance expression of a ligamentous phenotype in hASCs compared to collagen gel alone. LDM prepared using phosphate-buffered saline or 0.1% peracetic acid was mixed with collagen gel (COL) and was evaluated for its ability to induce proliferation, differentiation, and extracellular matrix synthesis in hASCs over 28 days in culture at different seeding densities (0, 0.25Â10 6 , 1Â106 , or 2Â10 6 hASC/mL). Biochemical and gene expression data were analyzed using analysis of variance. Fisher's least significant difference test was used to determine differences between treatments following analysis of variance. hASCs in either LDM or COL demonstrated changes in gene expression consistent with ligament development. hASCs cultured with LDM demonstrated more dsDNA content, sulfated-glycosaminoglycan accumulation, and type I and III collagen synthesis, and released more sulfated-glycosaminoglycan and collagen into the medium compared to hASCs in COL ( p 0.05). Increased seeding density increased DNA content incrementally over 28 days in culture for LDM but not COL constructs ( p 0.05). These findings suggest that LDM can stimulate a ligament phenotype by hASCs, and may provide a novel scaffold material for ligament engineering applications.

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Section: Little Et Almentioning

confidence: 99%

Ligament-Derived Matrix Stimulates a Ligamentous Phenotype in Human Adipose-Derived Stem Cells

Little

Guilak

Ruch

2010

Tissue Engineering Part A

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“…18 Fibroblast growth factors (FGFs) are a large family of polypeptide growth factors that regulate the secretion of various extracellular matrix (ECM) proteins, which contribute to the formation of new blood vessels. 19,20 Fibroblast growth factor 2 (FGF2) is a potent mitogen in a variety of cells and is effective in regulating the proliferation and ECM-related gene expression of fibroblasts, 21,22 the most common cell type in peri-implant gingival connective tissue.…”

Section: Introductionmentioning

confidence: 99%

Concentration- and time-dependent response of human gingival fibroblasts to fibroblast growth factor 2 immobilized on titanium dental implants

Wang²,

Chu³

et al. 2012

IJN

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Background: Titanium (Ti) implants are widely used clinically, but peri-implantitis remains one of the most common and serious complications. Healthy integration between gingival tissue and the implant surface is critical to long-term success in dental implant therapy. The objective of this study was to investigate how different concentrations of immobilized fibroblast growth factor 2 (FGF2) on the titania nanotubular surface influence the response of human gingival fibroblasts (HGFs). Methods: Pure Ti metal was anodized at 20 V to form a vertically organized titanium dioxide nanotube array on which three concentrations of FGF2 (250 ng/mL, 500 ng/mL, or 1000 ng/mL) were immobilized by repeated lyophilization. Surface topography was observed and FGF2 elution was detected using enzyme-linked immunosorbent assay. The bioactivity changes of dissolvable immobilized FGF2 were measured by methyl-thiazolyl-tetrazolium assay. Behavior of HGFs was evaluated using adhesion and methyl-thiazolyl-tetrazolium bromide assays. Results: The FGF2 remained for several days on the modified surface on which HGFs were cultured. Over 90% of the dissolvable immobilized FGF2 had been eluted by Day 9, whereas the FGF2 activity was found to diminish gradually from Day 1 to Day 9. The titania nanotubular surface with an optimal preparing concentration (500 ng/mL) of FGF2 immobilization exhibited improved HGF functions such as cellular attachment, proliferation, and extracellular matrix-related gene expression. Moreover, significant bidirectional as well as concentration-and time-dependent bioactivity was observed. Conclusion: Synergism of the FGF2-impregnated titanium dioxide nanotubular surface revealed good gingival-implant integration, indicating that these materials might have promising applications in dentistry and other biomedical devices.

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“…This suggests MMP-1 is associated with pro-angiogenic events (Blackburn and Brinckerhoff, 2008). Research regarding the effects of bFGF on MMP-1 expression has demonstrated that bFGF administration increase MMP-1 expression in human periodontal ligament cells (Palmon et al, 2000). An increase of MMP-1 protein was also observed when bFGF was added to human skin fibroblasts (Xie et al, 2008).…”

Section: Discussionmentioning

confidence: 99%

Cathepsin L derived from skeletal muscle cells transfected with bFGF promotes endothelial cell migration

Chung

Jin

et al. 2011

Exp Mol Med

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Inverse Dose‐ and Time‐Dependent Effect of Basic Fibroblast Growth Factor on the Gene Expression of Collagen Type I and Matrix Metalloproteinase‐1 by Periodontal Ligament Cells in Culture

Abstract: These results suggest that bFGF is one of the important regulators involved in the active remodeling of col I in the periodontal ligament and possibly in other connective tissues.

Cited by 46 publications

References 29 publications

Ligament-Derived Matrix Stimulates a Ligamentous Phenotype in Human Adipose-Derived Stem Cells

Ligament-Derived Matrix Stimulates a Ligamentous Phenotype in Human Adipose-Derived Stem Cells

Concentration- and time-dependent response of human gingival fibroblasts to fibroblast growth factor 2 immobilized on titanium dental implants

Cathepsin L derived from skeletal muscle cells transfected with bFGF promotes endothelial cell migration

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