The main goal of this paper is to propose an approach to inverse spectral problems for functional-differential operators (FDO) with involution. For definiteness, we focus on the second-order FDO with involution-reflection. Our approach is based on the reduction of the problem to the matrix form and on the solution of the inverse problem for the matrix Sturm-Liouville operator by developing the method of spectral mappings. The obtained matrix Sturm-Liouville operator contains the weight, which causes qualitative difficulties in the study of the inverse problem. As a result, we show that the considered FDO with involution is uniquely specified by five spectra of certain regular boundary value problems.