Invertase Activity and its Relation to Hexose Accumulation in Potato Tubers

Richardson, D.; Davies, Howard V.; Ross, Heather A.; Mackay, G. R.

doi:10.1093/jxb/41.1.95

Cited by 80 publications

(49 citation statements)

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“…The effect of genotype on the response is also reported. This complements work published previously on the influence of genotype on sugar accumulation in tubers stored at low temperature (21) and on sugar metabolism in developing tubers (15,23). Furthermore, whereas Claussen et al (2) suggested that sucrose regulates sucrose synthase activity, this hypothesis has not been adequately tested for starch-storing sink tissues such as potato tubers.…”

Section: 2126)supporting

confidence: 62%

“…Potatoes (Solanum tuberosum L.) cvs Cara, Record, and Brodick, were grown in the field at the Scottish Crop Research Institute as described previously (21 Lyophilized and finely ground samples were extracted in 80% ethanol at 70°C and the concentration of glucose, fructose, and sucrose determined using enzyme-coupled reaction systems (21). In 14C experiments, ethanol extracts were prepared as above and total 14C in the soluble fraction determined.…”

Section: Tuber Excision and Postharvest Storage Experimentsmentioning

confidence: 99%

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Sucrose Metabolism in Tubers of Potato (Solanum tuberosum L.)

Ross¹,

Davies²

1992

Plant Physiol.

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Excision of developing potato (Solanum tuberosum L.) tubers from the mother plant, followed by storage at 10°C, resulted in a rapid, substantial decrease in sucrose synthase activity and considerable increases in hexose content and acid invertase activity. A comparison of the response of three genotypes, known to accumulate different quantities of hexoses in storage, showed that both sucrose synthase activity and the extent to which activity declined following excision were similar in all cases. However, there was significant genotypic variation in the extent to which acid invertase activity developed, with tubers accumulating the highest hexose content also developing the highest extractable activity of invertase. Similar effects were found in nondetached tubers when growing plants were maintained in total darkness for a prolonged period. Furthermore, supplying sucrose to detached tubers through the cut stolon surface prevented the decline in sucrose synthase activity. Maltose proved to be ineffective. Westem blots using antibodies raised against maize sucrose synthase showed that the decline in sucrose synthase activity was associated with the loss of protein rather than the effect of endogenous inhibitors. Although there were indications that maintaining a flux of sucrose into isolated tubers could prevent the increase in acid invertase activity, the results were not conclusive.

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Section: 2126)supporting

confidence: 62%

Section: Tuber Excision and Postharvest Storage Experimentsmentioning

confidence: 99%

Sucrose Metabolism in Tubers of Potato (Solanum tuberosum L.)

Ross¹,

Davies²

1992

Plant Physiol.

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“…Low-temperature stress and other environmental stresses can induce essential changes in carbohydrate metabolism in plants (Gupta & Kaur 2005, Guy et al 2008. The role of sucrose in cold stress in plant tissues has been studied in detail with focus on the responses of related enzymes such as invertase (EC 3.2.1.26) (Richardson 1990), sucrosephosphate synthase (EC 2.4.1.14) and sucrose synthase (EC 2.4.1.13) (Guy et al 2008).…”

Section: Analysis Of Sugar Content and Expression Of Sucrose Transpormentioning

confidence: 99%

Analysis of Sugar Content and Expression of Sucrose Transporter Genes (<i>OsSUTs</i>) in Rice Tissues in Response to a Chilling Temperature

Takahashi

Meguro-Maoka

Yoshida

2017

JARQ

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Changes in sucrose metabolism have been implicated in tissue damage found in rice plants exposed to cold. However, little is known about the effect of low temperature on sucrose transport in cold-sensitive plants such as rice. Here, we investigated the changes induced by a 12°C chilling treatment on the soluble sugar content relative to the expression of sucrose transporter genes (OsSUT1-5) in various tissues of young (6-week-old) and mature (booting stage) rice plants. Sucrose levels increased in source tissues but decreased in young panicles during the chilling treatment. Sucrose transporter genes also responded to the chilling treatment: OsSUT1 in leaf sheaths and OsSUT2 and OsSUT4 in panicles were down-regulated. Our results suggested that the sucrose translocation supported by OsSUTs in rice plants decreased during chilling treatment, assuming the involvement of OsSUT1 and OsSUT4 in phloem loading of sucrose. These changes may induce a sugar imbalance in anthers, resulting in impaired pollen development.

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“…Ao serem processados na forma de "chips", em altas temperaturas durante a fritura, são formados produtos de coloração escura. A cor desenvolvida durante a fritura é atribuída à reação de Maillard (escurecimento não enzimático) que ocorre quando o grupo carbonila do carboidrato interage com o grupo amino do aminoácido ou proteína, e após várias etapas produz as melanoidinas, depreciando ou, em alguns casos, até impedindo a comercialização do produto (RICHARDSON et al, 1990). Embora este fenômeno tenha sido reconhecido há séculos, o entendimento do mecanismo responsável pelo adoçamento é incompleto.…”

Section: Introductionunclassified

“…Provavelmente, a sequência pela qual o acúmulo de açúcares ocorre é iniciada com a mobilização do amido, seguida por uma aumentada síntese de sacarose e, finalmente, hidrólise da sacarose até glicose (AP REES et al, 1981). Este passo é afirmado pela quase equimolaridade de glicose e frutose durante o acúmulo de açúcares induzido pelo frio (RICHARDSON et al, 1990). Devido à equimolaridade observada entre glicose e frutose e sua igual relevância para a reação de Maillard, as duas formas de açúcares redutores são tratadas como um composto.…”

Section: Introductionunclassified