Invertase, sucrose synthase and sucrose phosphate synthase in lyophilized spruce needles; microplate reader assays

Egger, B.; Hampp, Rüdiger

doi:10.1007/bf00225476

Cited by 36 publications

(40 citation statements)

References 18 publications

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“…In winter, pineapple had a long growth stage, so can accumulate more sugar than that in summer. Meanwhile, the changes of activity of SS were similar with SPS (Egger and Hampp, 1993).…”

Section: Discussionsupporting

confidence: 53%

Dynamic analysis of sugar metabolism in different harvest seasons of pineapple (Ananas comosus L. (Merr.)

Zhang¹,

Dou²,

Yao³

et al. 2011

Afr. J. Biotechnol.

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In pineapple fruits, sugar accumulation plays an important role in flavor characteristics, which varies according to the stage of fruit development. Metabolic changes in the contents of fructose, sucrose and glucose and reducing sugar related to the activities of soluble acid invertase (AI), neutral invertase (NI), sucrose synathase (SS) and sucrose-phosphate synthase (SPS) were studied in winter and summer pineapple fruits in this paper. Sucrose was significantly increased in most of the harvesting winter fruits which reached the peak of 64.87 mg·g-1 FW at 130 days after anthesis, while hexose was mainly accumulated at the 90 day of the summer fruits in July. The ratio of hexose to sucrose was 5.92:0.73 from the winter fruit in February. Interestingly, the activities of SPS and SS synthetic direction of the harvested fruits in February were significantly higher than those in July, whereas the invertase activities were exactly opposite. NI activity showed a similar trend to AI, but the amount of NI activity was higher than AI in both months. Therefore, NI appears to be one of the vital enzymes in pineapple fruit development. Conclusively, the enzyme activities related to sugar play key roles in the eating of quality pineapple, which could be improved by cultivation in different seasons. So we can arbitrate different temperature to improve the quality of pineapple fruits according to market demand.

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“…In winter, pineapple had a long growth stage, so can accumulate more sugar than that in summer. Meanwhile, the changes of activity of SS were similar with SPS (Egger and Hampp, 1993).…”

Section: Discussionsupporting

confidence: 53%

Dynamic analysis of sugar metabolism in different harvest seasons of pineapple (Ananas comosus L. (Merr.)

Zhang¹,

Dou²,

Yao³

et al. 2011

Afr. J. Biotechnol.

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“…Extractable activities of sucrose phosphate synthase, sucrose synthase, acid (pH 3-8) and alkaline invertase (pH 6-8) were measured using a microplate photometer according to Egger & Hampp (1993). ADPglucose pyrophosphorylase and starch phosphorylase were determined as described by Egger et al (1996).…”

Section: Methodsmentioning

confidence: 99%

Ozone‐induced accumulation of carbohydrates changes enzyme activities of carbohydrate metabolism in birch leaves

et al. 1997

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SUMMARYBirch cuttings {Betula pendula Roth) were grown in a sand-culture system with two concentrations (0-05, HF and 0-005%, LF) of fertilizer containing macronutrients and micronutrients, and were exposed to 90/40 nll"^ O3 (day/night) and < 3 nl T^ O3 (control) for one growing season in the field fumigation chambers at Birmensdorf (Switzerland). Leaves of different ages were analysed for gas exchange, contents of chlorophyll, protein, and for metabolites as well as enzyme activities of carbohydrate metabolism.Ozone reduced net photosynthesis and chlorophyll contents in mature leaves of both fertilization treatments, whereas that of protein was only reduced in high-fertilized plants (HF). However, net photosynthesis, chlorophyll, and protein increased in young leaves of low'-fertilized plants (LF). The effects of ozone on enzyme activities of carbohydrate metabolism were most pronounced in leaves of LF plants. Specific activities of the sucrose-cleaving enzymes, sucrose synthase and alkaline invertase, were induced, whereas acid invertase was unchanged. Extractable activity of sucrose phosphate synthase, which is a key enzyme of sucrose synthesis, was reduced. Levels of fructose 2,6-bisphosphate, an inhibitor of sucrose synthesis, were increased in leaves of O3/LF plants, but reduced in O3/HF plants. In addition, activities of enzymes involved in starch metabolism, ADP-glucose pyrophosphorylase and starch phosphorylase, were lowered in ozone-treated samples and the ratio of ATP: ADP was increased.It is concluded that chronic ozone exposure leads to an inhibition of sucrose synthesis and favours sucrose degradation. This effect is modulated by the nutrient status of the plants, indicating higher O3 tolerance in HF plants. Furthermore, as the metabolic responses in the ozone-treated samples resemble very closely those observed under end-product inhibition of photosynthesis, we assume that the O3 effect is mainly due to reduced photosynthate export.

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“…Enzymes such as sucrose invertase are involved in this process (Tymowska-Lalanne and Kreis, 1998), and alkaline/neutral invertase (NI) is involved in sucrose hydrolysis in the cytoplasm (Egger and Hampp, 1993;Du et al, 2010). NI regulates sucrose metabolism in many plants, including Saccharum officinarum, Beta vulgaris, and Citrus reticulata (Hubbard et al, 1991), and plays an important role in monosaccharide-dependent biosynthesis (Nonis et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Characterization of the alkaline/neutral invertase gene in Dendrobium officinale and its relationship with polysaccharide accumulation

Gao¹,

Xf²,

Jp³

et al. 2016

Genet. Mol. Res.

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ABSTRACT. Dendrobium officinale is one of the most wellknown traditional Chinese medicines, and polysaccharide is its main active ingredient. Many studies have investigated the synthesis and accumulation mechanisms of polysaccharide, but until recently, little was known about the molecular mechanism of how polysaccharide is synthesized because no related genes have been cloned. In this study, we cloned an alkaline/neutral invertase gene from D. officinale (DoNI) by the rapid amplification of cDNA ends (RACE) method. DoNI was 2231 bp long and contained an open reading frame that predicted a 62.8-kDa polypeptide with 554-amino acid residues. An alkaline/neutral invertase conserved domain was predicted from this deduced amino acid sequence, and DoNI had a similar deduced amino acid sequence to Setaria italica and Oryza brachyantha. We also found that DoNI expression in different tissues was closely related to DoNI activity, and more importantly, polysaccharide level. Our results indicate that DoNI is associated with polysaccharide accumulation in D. officinale.

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Invertase, sucrose synthase and sucrose phosphate synthase in lyophilized spruce needles; microplate reader assays

Cited by 36 publications

References 18 publications

Dynamic analysis of sugar metabolism in different harvest seasons of pineapple (Ananas comosus L. (Merr.)

Dynamic analysis of sugar metabolism in different harvest seasons of pineapple (Ananas comosus L. (Merr.)

Ozone‐induced accumulation of carbohydrates changes enzyme activities of carbohydrate metabolism in birch leaves

Characterization of the alkaline/neutral invertase gene in Dendrobium officinale and its relationship with polysaccharide accumulation

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