Invertebrate RNA virus diversity from a taxonomic point of view

Ryabov, Eugene V.

doi:10.1016/j.jip.2016.10.002

Cited by 42 publications

(32 citation statements)

References 106 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The RNAi pathway has a major role in antiviral immunity in insects, protecting them from viral infections or maintaining hemostasis with viral infections . Next‐generation sequencing has provided evidence that insects harbor a wide range of insect‐specific RNA viruses, and these viruses are often restricted by the RNAi pathway . Aphids feed on plant phloem, therefore, their viral infections occur mainly through two routes, digestive system and/or body wall.…”

Section: Discussionmentioning

confidence: 99%

Topical dsRNA delivery induces gene silencing and mortality in the pea aphid

Niu

Yang

Tian

et al. 2019

Pest Management Science

View full text Add to dashboard Cite

BACKGROUND: With the growing number of available aphid genomes and transcriptomes, an efficient and easy-to-adapt tool for gene function study is urgently required. RNA interference (RNAi), as a post-transcriptional gene silencing mechanism, is important as a research tool for determining gene functions and has potential as a novel insect control strategy. However, these applications have been hampered by the lack of effective dsRNA delivery approaches in aphids. RESULTS:Here, we developed a convenient and efficient dsRNA delivery method, topical RNAi, in aphids. An investigation of its dose and time-dependent RNAi efficiencies revealed that with as little as 60 ng dsRNA per adult pea aphid (Acyrthosiphon pisum), the indicator gene, Aphunchback, could be significantly silenced within 2 h of exposure. The method was further validated by successfully silencing other different genes, and it was also efficient toward two other aphid species, Aphis citricidus and Myzus persicae. Furthermore, a noticeable mortality was also observed in pea aphids using topical RNAi-mediated gene silencing, within 4 days post-dsRNA application for four out of seven tested genes. CONCLUSION: Compared with the currently used dsRNA delivery methods in aphids, microinjection and ingestion, topical RNAi is time-and cost-effective, which could greatly influence RNAi-based gene functional studies and potential candidate gene selection for developing RNAi-based aphid control strategies in the future.

show abstract

Section: Discussionmentioning

confidence: 99%

Topical dsRNA delivery induces gene silencing and mortality in the pea aphid

Niu

Yang

Tian

et al. 2019

Pest Management Science

View full text Add to dashboard Cite

show abstract

“…RNA viruses infecting invertebrates belong to three of the four RNA virus orders: Mononegavirales (two families out of five), Nidovirales (two families out of five), and Picornavirales (two families out of five) and ten out of 37 RNA virus families, that have yet to be assigned to an order (Ryabov, 2017). The order Nidovirales is of importance here, as it comprises the vertebrate-specific family Coronaviridae, to which SARS-CoV, MERS-CoV and SARS-CoV-2 belong, as well two other families, the Mesoniviridae and Roniviridae occurring in mosquitoes and shrimps, respectively (ICTV, 2011b).…”

Section: Viruses In the Microbiomes Of Edible Insectsmentioning

confidence: 99%

Edible insects unlikely to contribute to transmission of coronavirus SARS-CoV-2

Dicke

Eilenberg

Salles³

et al. 2020

Journal of Insects as Food and Feed

View full text Add to dashboard Cite

In the context of food safety, edible insects are evaluated for biological hazards such as microbial pathogens according to regulations currently in place. When the European Food Safety Authority evaluated the hazards of edible insects as a potential source of pathogenic viruses for humans and livestock, the novel zoonotic coronavirus SARS-CoV-2 had not yet emerged but other pathogenic coronaviruses such as SARS (SARS-CoV) and MERS (MERS-CoV) were known. As a result of the COVID-19 pandemic, animal sources of protein for human consumption are being evaluated for the risks of being a transmission vector of coronaviruses, like SARS-CoV-2. Insects lack a receptor that can bind SARS-CoV-2, thus preventing the virus from replicating in insects, unlike some vertebrate livestock species and companion animals. Despite extensive monitoring, coronaviruses have never been recorded in insect microbiomes. Contamination of insects produced for food or feed may occur during the production process, resulting from rearing substrate or from insect farmers. However, the currently permitted rearing substrates do not include animal products and the farming process is highly automated, thus limiting interactions between farmers and insects. If contamination would still occur, the fact that the insects in production are not hosts to SARS-CoV-2 precludes virus replication and the further processing of the insects will destroy the contamination. We conclude that the hazard of edible insects being a transmission vector of SARS-CoV-2 is extremely low.

show abstract

“…This could be expected from viruses that integrate their genome into that of their host as an essential step of their life cycle, like retroviruses. However, many EVEs derive from the viruses displaying no DNA stage, and it appears that all kinds of viruses end up in genomes, although the mechanisms involved are generally unknown [ 7 , 9 , 10 ]. Most of the time, after their integration, EVEs slowly decay under neutral molecular evolution, because they are no longer active.…”

Section: Introductionmentioning

confidence: 99%

Impact of Lateral Transfers on the Genomes of Lepidoptera

Drezen

Josse

Bézier

et al. 2017

Genes

View full text Add to dashboard Cite

Transfer of DNA sequences between species regardless of their evolutionary distance is very common in bacteria, but evidence that horizontal gene transfer (HGT) also occurs in multicellular organisms has been accumulating in the past few years. The actual extent of this phenomenon is underestimated due to frequent sequence filtering of “alien” DNA before genome assembly. However, recent studies based on genome sequencing have revealed, and experimentally verified, the presence of foreign DNA sequences in the genetic material of several species of Lepidoptera. Large DNA viruses, such as baculoviruses and the symbiotic viruses of parasitic wasps (bracoviruses), have the potential to mediate these transfers in Lepidoptera. In particular, using ultra-deep sequencing, newly integrated transposons have been identified within baculovirus genomes. Bacterial genes have also been acquired by genomes of Lepidoptera, as in other insects and nematodes. In addition, insertions of bracovirus sequences were present in the genomes of certain moth and butterfly lineages, that were likely corresponding to rearrangements of ancient integrations. The viral genes present in these sequences, sometimes of hymenopteran origin, have been co-opted by lepidopteran species to confer some protection against pathogens.

show abstract

Invertebrate RNA virus diversity from a taxonomic point of view

Cited by 42 publications

References 106 publications

Topical dsRNA delivery induces gene silencing and mortality in the pea aphid

Topical dsRNA delivery induces gene silencing and mortality in the pea aphid

Edible insects unlikely to contribute to transmission of coronavirus SARS-CoV-2

Impact of Lateral Transfers on the Genomes of Lepidoptera

Contact Info

Product

Resources

About