Investigating bioconjugation by atomic force microscopy

Teßmer, Ingrid; Kaur, Parminder; Lin, Jiangguo; Wang, Hong

doi:10.1186/1477-3155-11-25

Cited by 21 publications

(13 citation statements)

References 109 publications

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“…This characteristics provides the flexibility in loading drugs and ligands to nanoparticles. [176] Biotin and avidin are also readily available with various functional groups for chemical conjugations. Moreover, the chemical conjugation on biotin or avidin avoids direct modification of the active biomolecule, thus maintaining their activity.…”

Section: Discussionmentioning

confidence: 99%

The principles and applications of avidin-based nanoparticles in drug delivery and diagnosis

Jain

Cheng

2017

Journal of Controlled Release

227

286

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Avidin-biotin interaction is one of the strongest non-covalent interactions in the nature. Avidin and its analogues have therefore been extensively utilized as probes and affinity matrices for a wide variety of applications in biochemical assays, diagnosis, affinity purification, and drug delivery. Recently, there has been a growing interest in exploring this non-covalent interaction in nanoscale drug delivery systems for pharmaceutical agents, including small molecules, proteins, vaccines, monoclonal antibodies, and nucleic acids. Particularly, the ease of fabrication without losing the chemical and biological properties of the coupled moieties makes the avidin-biotin system a versatile platform for nanotechnology. In addition, avidin-based nanoparticles have been investigated as diagnosis systems for various tumors and surface antigens. In this review, we will highlight the various fabrication principles and biomedical applications of avidin-based nanoparticles in drug delivery and diagnosis. The structures and biochemical properties of avidin, biotin and their respective analogues will also be discussed.

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Section: Discussionmentioning

confidence: 99%

The principles and applications of avidin-based nanoparticles in drug delivery and diagnosis

Jain

Cheng

2017

Journal of Controlled Release

227

286

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“…In addition, also atomic force microscopy (AFM) can be extremely useful in nanosurgery applications, by taking advantage of its bioconjugation capabilities coupled with active assembly and analysis of bioconjugation-based nanostructures [104]. In particular, in [103] the authors have proposed an AFM-based nanorobot with integrated imaging, manipulation, analysis, and tracking functions, important for cellular-level surgery on live samples.…”

Section: Nanosurgerymentioning

confidence: 99%

Applications of molecular communications to medicine: A survey

Felicetti

Femminella

Reali

et al. 2016

Nano Communication Networks

151

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In recent years, progresses in nanotechnology have established the foundations for implementing nanomachines capable of carrying out simple but significant tasks. Under this stimulus, researchers have been proposing various solutions for realizing nanoscale communications, considering both electromagnetic and biological communications. Their aim is to extend the capabilities of nanodevices, so as to enable the execution of more complex tasks by means of mutual coordination, achievable through communications.However, although most of these proposals show how devices can communicate at the nanoscales, they leave in the background specific applications of these new technologies. Thus, this paper shows an overview of the actual and potential applications that can rely on a specific class of such communications techniques, commonly referred to as molecular communications. In particular, we focus on health-related applications. This decision is due to the rapidly increasing interests of research communities and companies to minimally invasive, biocompatible, and targeted health-care solutions. Molecular communication techniques have actually the potentials of becoming the main technology for implementing advanced medical solution.Hence, in this paper we provide a taxonomy of potential applications, illustrate them in some details, alongwith the existing open challenges for them to be actually deployed, and draw future perspectives.

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“…Recently, several fluorogenic or cell-specific RNA aptamers have been developed for live cell applications (4). Bioconjugation of RNA aptamers with nanoparticles could combine unique and orthogonal strengths of the specific interactions of RNA nanostructures and novel material properties of nanoparticles and open up new options for a wide range of applications (10). For example, the utility of SAXS, AFM for analysis of nanoparticle-conjugated RNA nanostructures could also be established (13).…”

Section: Discussionmentioning

confidence: 99%

“…Due to their amenability of synthesis, unique surface, high electron density and strong optical absorption, gold nanoparticles (AuNPs) are an obvious choice in many biomedical research (8). For example, AuNPs have been used widely for labeling proteins for molecular localization in electron microscopy (9), or as markers to directly visualize the structure and conformational changes of biomolecules by atomic force microscopy (AFM) imaging (10).…”

Section: Introductionmentioning

confidence: 99%

Site-specific covalent labeling of large RNAs with nanoparticles empowered by expanded genetic alphabet transcription

Wang¹,

Chen

Hu³

2020

Preprint

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Classification Biological Sciences/Biochemistry; Physical Sciences/ChemistryKeywords Site-specific nanoparticle labeling, expanded genetic alphabet, TPT3-NaM, large RNAs, X-ray scattering interferometry AbstractConjugation of RNAs with nanoparticles is of significant importance for its numerous applications in biology and medicine, which however remains challenging, especially for large ones. So far, the majority of RNA labeling rely on solid-phase chemical synthesis, which is generally limited to RNAs smaller than 100 nts. We here present an efficient and generally applicable labeling strategy for site-specific covalent conjugation of large RNAs with gold nanoparticle (AuNP) empowered by expanded genetic alphabet transcription. We synthesize an amine-derivatized TPT3 (TPT3 A ), which are site-specifically incorporated into a 97-nt 3'SL RNA and a 719-nt mini genomic RNA (DENV-mini) from Dengue virus serotype 2 (DENV2) by standard in vitro transcription with expanded genetic alphabet containing the A-T, G-C natural base pairs and the TPT3-NaM unnatural base pair. TPT3 modification cause minimal structural perturbations to the RNAs by small angle X-ray scattering. The purified TPT3 A -modified RNAs are covalently conjugated with mono-Sulfo-NHS-Nanogold nanoparticles via the highly selective amine-NHS ester reaction and purified under non-denaturing conditions. We demonstrate the application of the AuNP-RNA conjugates in large RNA structural biology by an emerging molecular ruler, X-ray scattering interferometry (XSI). The inter-nanoparticle distance distributions in the 3'SL and DENV-mini RNAs derived from XSI measurements support the hypothetical model of flavivirus genome circularization, thus validate the applicability of this novel labeling strategy. The presented strategy overcomes the size constraints in conventional RNA labeling strategies, and is expected to have wide applications in large RNA structural biology and RNA nanotechnology. Significance StatementWe present a site-specific labeling strategy for large RNAs by T7 transcription with expanded genetic alphabet containing TPT3-NaM unnatural base pair. The applicability of this labeling strategy is validated by X-ray scattering interferometry measurements on a 97-nt and a 719-nt RNAs. This strategy can be applicable to natural RNAs or artificial RNA nanostructures with sizes from tens up to thousands of nucleotides, or covalent conjugation of RNAs with other metal nanoparticles. The usage of a far upstream forward primer during PCR enables easy purification of RNA from DNA templates, the non-denaturing conditions for conjugation reactions and purification avoids potential large RNA misfolding. This labeling strategy expands our capability to site-specifically conjugate RNA with nanoparticles for many applications. Main Text

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Investigating bioconjugation by atomic force microscopy

Cited by 21 publications

References 109 publications

The principles and applications of avidin-based nanoparticles in drug delivery and diagnosis

The principles and applications of avidin-based nanoparticles in drug delivery and diagnosis

Applications of molecular communications to medicine: A survey

Site-specific covalent labeling of large RNAs with nanoparticles empowered by expanded genetic alphabet transcription

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