Investigating conformational changes of Prefoldin β1 as result of applying external mechanical force without any position constraint

Abstract

Manipulating molecular scale bio-nanorobots and influencing their behaviour is one of the major challenges of new researches. Many coiled coil type proteins are involved in important biological functions due to physical properties that make them ideal for both nanoscale manipulation and sensing. The Prefoldin beta subunit from Thermococcus strain KS-1(Prefoldin β1) is one of the possible proteins that can serve as a new bio-nano-actuator. Besides having a balanced architecture, Prefoldin β1 can exhibit a wide range of exclusive authorities. In this study, steered molecular dynamics simulation is applied along with the centre of mass pulling and analyses of Prefoldin β1 conformational changes to characterise some of those abilities. Thus, applying external mechanical force without any position constraint shows that it has no movement throughout simulations. This proposes a novel method to capture different sizes and shapes of cargoes. During simulations, each arm was found to be very flexible, allowing it to enlarge its central cavity and capture different cargoes. For a more accurate analysis, the variations in the cavity of nano-actuator are investigated qualitatively and quantitatively with different parameters. Also, the force analysis of the arms can provide us with decent information about the performance of this nano-actuator.

Motion Trajectory Error of Robotic Arm Based on Neural Network Algorithm

“…In this method, the controller is created according to the input and data of the manipulator process without model data specifcation. Te simulation results show that the error convergence is zero, and the convergence efect is better [5]. A feedforward dynamic position feedback controller, a variable matrix nonlinear controller, and a frst-order closed-loop linear dynamic compensator are studied for the control of a speedsensitive manipulator.…”