2024
DOI: 10.3390/molecules29215161
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Investigating Flavonoids by HPTLC Analysis Using Aluminium Chloride as Derivatization Reagent

Sharmin Sultana,
Md Lokman Hossain,
Tom Sostaric
et al.

Abstract: This is the first study to report on high performance thin layer chromatography (HPTLC) generated spectrophotometric data to systematically capture flavonoid compounds using optimized derivatization with either AlCl3 or NaNO2-AlCl3-NaOH as visualisation reagents. While the traditional AlCl3 colorimetric method using UV–Vis analysis provides valuable insights into the presence of flavonoids and allows derivation of the total flavonoid content (TFC) of a sample, HPTLC fingerprints obtained after spraying with Al… Show more

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