Investigating host-pathogen meta-metabolic interactions of Magnaporthe oryzae infected barley using infrared matrix-assisted laser desorption electrospray ionization mass spectrometry

Kalmar, Jaclyn Gowen; Oh, Yeonyee; Dean, Ralph A.; Muddiman, David C.

doi:10.1007/s00216-019-02216-z

Cited by 5 publications

(4 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, by applying ion mobility separations prior to HRMS detection, there is the potential to resolve isomeric compounds, without prior chromatographic separation. Recent salient MSi examples include the barley defense metabolite serotonin and other metabolites of the melanin pathway against M. oryzae [ 166 ], and the sulfur-containing glucosinolates in Arabidopsis, which intervene in fungal and pest responses [ 133 ]. Besides MSi, other approaches allowing the isolation of cells and organelles (e.g., flow cytometry, cell and organelle sorting, microfluidics) will make an outstanding contribution to the future impacts of metabolomics on plant stress studies.…”

Section: Future Prospects: Which New Tools and Approaches Will Make An Impact?mentioning

confidence: 99%

Unravelling Plant Responses to Stress—The Importance of Targeted and Untargeted Metabolomics

et al. 2021

View full text Add to dashboard Cite

Climate change and an increasing population, present a massive global challenge with respect to environmentally sustainable nutritious food production. Crop yield enhancements, through breeding, are decreasing, whilst agricultural intensification is constrained by emerging, re-emerging, and endemic pests and pathogens, accounting for ~30% of global crop losses, as well as mounting abiotic stress pressures, due to climate change. Metabolomics approaches have previously contributed to our knowledge within the fields of molecular plant pathology and plant–insect interactions. However, these remain incredibly challenging targets, due to the vast diversity in metabolite volatility and polarity, heterogeneous mixtures of pathogen and plant cells, as well as rapid rates of metabolite turn-over. Unravelling the systematic biochemical responses of plants to various individual and combined stresses, involves monitoring signaling compounds, secondary messengers, phytohormones, and defensive and protective chemicals. This demands both targeted and untargeted metabolomics approaches, as well as a range of enzymatic assays, protein assays, and proteomic and transcriptomic technologies. In this review, we focus upon the technical and biological challenges of measuring the metabolome associated with plant stress. We illustrate the challenges, with relevant examples from bacterial and fungal molecular pathologies, plant–insect interactions, and abiotic and combined stress in the environment. We also discuss future prospects from both the perspective of key innovative metabolomic technologies and their deployment in breeding for stress resistance.

show abstract

Section: Future Prospects: Which New Tools and Approaches Will Make An Impact?mentioning

confidence: 99%

Unravelling Plant Responses to Stress—The Importance of Targeted and Untargeted Metabolomics

et al. 2021

View full text Add to dashboard Cite

show abstract

“…Previous research has shown that the F-box E3 ligase protein in Magnaporthe oryzae is found to be crucial for its growth and pathogenicity [26]. MoGrr1 is another F-box protein that participates in conidiogenesis, maintenance of cell wall integrity and viral pathogenicity of M. oryzae [27].…”

Section: Discussionmentioning

confidence: 99%

A putative F‐box‐domain‐encoding gene AOL_s00076g207 regulates the development and pathogenicity of Arthrobotrys oligospora

Peng

Dong

et al. 2021

J Basic Microbiol

View full text Add to dashboard Cite

F-box protein is a key component of the Skp1-cullin-F-box-type ubiquitin ligase complex (SCF-ULC) that marks its target proteins with ubiquitin for proteasomal degradation. In this study, we explored the potential role of AOL_s00076g207 (Aog207) in Arthrobotrys oligospora, a model fungus for studying nematodes-fungi interactions. The Aog207 gene encodes a putative F-box protein of the SCF-ULC. Deletion of Aog207 could inhibit mycelial growth in TYGA and PDA media. More importantly, the conidial germination rate of ΔAog207 mutants was remarkably declined compared to that of wildtype (WT) strain, and the mutant strains were more sensitive toward chemical stressors than the WT strain. In addition, ΔAog207 mutants generated fewer traps and captured fewer nematodes than WT strain. In summary, Aog207 disruption significantly affected the pathogenicity, mycelial growth, conidial germination, environmental adaptation and trap formation of A. oligospora.These findings may facilitate a better understanding of the nematode predation mechanism of A. oligospora and provide an experimental basis for developing biological control agents against nematodes.

show abstract

“…In short, the present technique does not yet appear suitable for application in widespread screening of diverse cyanobacterial cultures, unless the search is aimed at identifying substantial producers of a specific tolyporphin (i.e., of known mass). IR-MALDESI-MS has been used to measure analytes in plants, including Artemisia annua [ 147 ], Arabidopsis thaliana seedlings [ 148 ], fungal ( Magnaporthe oryzae )-infected barley [ 149 ], and maize [ 150 ]; in plant products, including cucumbers [ 151 ] and cherry tomatoes [ 152 ]; and in surrogate samples related to plants, including β-carotene [ 146 ]. The IR-MALDESI-FTMS method remains under rapid development.…”

Section: Quantitative Analysis Of Tolyporphinsmentioning

confidence: 99%

Tolyporphins–Exotic Tetrapyrrole Pigments in a Cyanobacterium—A Review

Nguyen,

Zhang,

Liu

et al. 2023

Molecules

View full text Add to dashboard Cite

Tolyporphins were discovered some 30 years ago as part of a global search for antineoplastic compounds from cyanobacteria. To date, the culture HT-58-2, comprised of a cyanobacterium–microbial consortium, is the sole known producer of tolyporphins. Eighteen tolyporphins are now known—each is a free base tetrapyrrole macrocycle with a dioxobacteriochlorin (14), oxochlorin (3), or porphyrin (1) chromophore. Each compound displays two, three, or four open β-pyrrole positions and two, one, or zero appended C-glycoside (or –OH or –OAc) groups, respectively; the appended groups form part of a geminal disubstitution motif flanking the oxo moiety in the pyrroline ring. The distinct structures and repertoire of tolyporphins stand alone in the large pigments-of-life family. Efforts to understand the cyanobacterial origin, biosynthetic pathways, structural diversity, physiological roles, and potential pharmacological properties of tolyporphins have attracted a broad spectrum of researchers from diverse scientific areas. The identification of putative biosynthetic gene clusters in the HT-58-2 cyanobacterial genome and accompanying studies suggest a new biosynthetic paradigm in the tetrapyrrole arena. The present review provides a comprehensive treatment of the rich science concerning tolyporphins.

show abstract

Investigating host-pathogen meta-metabolic interactions of Magnaporthe oryzae infected barley using infrared matrix-assisted laser desorption electrospray ionization mass spectrometry

Cited by 5 publications

References 37 publications

Unravelling Plant Responses to Stress—The Importance of Targeted and Untargeted Metabolomics

Unravelling Plant Responses to Stress—The Importance of Targeted and Untargeted Metabolomics

A putative F‐box‐domain‐encoding gene AOL_s00076g207 regulates the development and pathogenicity of Arthrobotrys oligospora

Tolyporphins–Exotic Tetrapyrrole Pigments in a Cyanobacterium—A Review

Contact Info

Product

Resources

About