Investigating hybridization in the parthenogenetic New Zealand stick insect Acanthoxyla (Phasmatodea) using single-copy nuclear loci

Buckley, Thomas R.; Attanayake, Dilini; Park, Duckchul; Ravindran, Shanthinie; Jewell, Tony; Normark, Benjamin B.

doi:10.1016/j.ympev.2008.02.016

Cited by 29 publications

(63 citation statements)

References 52 publications

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“…Point mutations present in clones but absent (without any evidence for double peaks) from direct sequencing were interpreted as polymerase errors, as were a few observed mutations resulting in stop codons, and restored to the state observed in the direct sequence. This is one of two sound procedures used in the few studies that take into account polymerase errors to assess allelic variation in nonmodel organisms (e.g., Buckley et al 2008;Schwander et al 2011;Harpke et al 2013). A more rigorous but less cost-effective-and, therefore, not so frequently usedalternative duplicates both PCR and cloning experiments to purge inconsistencies (e.g., Lukas and Vigilant 2005;Schwander et al 2011).…”

Section: Sequence Edition and Refinementmentioning

confidence: 99%

Nonrandom Patterns of Genetic Admixture Expose the Complex Historical Hybrid Origin of Unisexual Leaf Beetle Species in the GenusCalligrapha

Montelongo

Gómez‐Zurita²

2015

The American Naturalist

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Many unisexual animal lineages supposedly arose from hybridization. However, support for their putative hybrid origins mostly comes from indirect methodologies, which are rarely confirmatory. Here we provide compelling data indicating that tetraploid unisexual Calligrapha are true genetic mosaics obtained via analysis of mitochondrial DNA (mtDNA) and allelic variation and coalescence times for three single-copy nuclear genes (CPS, HARS, and Wg) in five of six unisexual Calligrapha and a representative sample of bisexual species. Nuclear allelic diversity in unisexuals consistently segregates in the gene pools of at least two but up to three divergent bisexual species, interpreted as putative parentals of interspecific hybridization crosses. Interestingly, their mtDNA diversity derives from an additional yet undiscovered older evolutionary lineage that is possibly the same for all independently originated unisexual species. One possibly extinct species transferred its mtDNA to several evolutionary lineages in a wave of hybridization events during the Pliocene, whereby descendant species retained a polymorphic mtDNA constitution. Recent hybridizations, in the Pleistocene and always involving females with the old introgressed mtDNA, seemingly occurred in the lineages leading to unisexual species, decoupling mtDNA introgression (and inferences derived from these data, such as timing and parentage) from subsequent acquisition of the new reproductive mode. These results illuminate an unexpected complexity in possible routes to animal unisexuality, with implications for the interpretation of ancient unisexuality. If the origin of unisexuality requires a mechanism where (1) hybridization is a necessary but insufficient condition and (2) multiple bouts of hybridization involving more than two divergent lineages are required, then the origins of several classical unisexual systems may have to be reassessed.

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Section: Sequence Edition and Refinementmentioning

confidence: 99%

Nonrandom Patterns of Genetic Admixture Expose the Complex Historical Hybrid Origin of Unisexual Leaf Beetle Species in the GenusCalligrapha

Montelongo

Gómez‐Zurita²

2015

The American Naturalist

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“…Chromosome counts suggest they are diploid, but nuDNA suggests that some Acanthoxyla lineages might be triploid (Buckley et al 2008). Sequence data indicate that all Acanthoxyla are of hybrid origin (Morgan-Richards & Trewick 2005;Buckley et al 2008) but whether C. hookeri is the original paternal species, or involved in a more recent hybridisation with some lineages of Acanthoxyla, is in question. The ancestral sexual maternal species of Acanthoxyla is probably extinct (Trewick et al 2008).…”

Section: Hybrid Speciesmentioning

confidence: 99%

A review of genetic analyses of hybridisation in New Zealand

Morgan‐Richards

Smissen

Shepherd

et al. 2009

Journal of the Royal Society of New Zealand

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Hybridisation between related taxa has a range of possible biological consequences, ranging from the production of sterile offspring, through introgression of alleles into populations, to the formation of new species. Examples of plant and animal species hybridising with related taxa abound in the New Zealand region. We review New Zealand examples of hybridisation that have been verified with chromosomal, protein or DNA data. Contemporary hybridisation has been studied at hybrid zones where distinct populations meet and mate in a defined and stable zone of contact. The role of human habitat modification is highlighted with examples of recent range changes that have led to hybridisation and subsequent conservation problems. Hybridisation can result in the swamping of endangered species, although it can also act as a bridge for the transfer of adaptations among lineages. Historical hybridisation in New Zealand has been examined with phylogenetics and there are many examples of organelle introgression or capture. The origin of new species of New Zealand stick insects, ferns and daisies via hybridisation has been demonstrated with cytogenetic and DNA sequence evidence. Thus the importance of hybridisation in the evolution of New Zealand's flora and fauna is highlighted.

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“…36 DNA sequencing and phylogenetic analysis We extracted DNA from the mermithid specimen that emerged from an individual of Clitarchus hookeri collected from Lake Okataina. DNA extraction methods followed Buckley et al (2008). PCR amplifications were performed using 25 ul reaction volumes containing about 5 ng of genomic DNA, 2.5 ul PCR buffer with MgCl 2 (Roche, USA), 200 uM each dNTR 1 ul BSA (10 mg/ml), 1 ul of 10 uM each prim er, 1.5 U of Fast start Taq DNA polym erase (Roche, USA).…”

Section: Morphological Analysesmentioning

confidence: 99%

“…PCR cycle conditions were 95°C for 4 min, followed by 40 cycles of 94°C for 1 mm, 48°C for 1 mm, 72°C for 1.5 min, and a final extension of 72°C for 10 min. The resulting template was purified and sequenced following Buckley et al (2008).…”

Section: Morphological Analysesmentioning

confidence: 99%

First records of mermithid nematodes (Nematoda: Mermithidae) parasitising stick insects (Insecta: Phasmatodea)

Yeates¹,

Buckley²

2009

New Zealand Journal of Zoology

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Hitherto the occurrence of mermithid nematodes (Nematoda: Mermithidae) parasitising stick insects has not been reported. Six mermithids were collected after emerging from specimens of New Zealand stick insects field collected and held in captivity. The mermithid nematodes are recorded from the stick insect genera Clitarchus Stål, Acanthoxyla Uvarov, Asteliaphasma Jewell & Brock and Tectarchus Salmon. Small subunit RNA (18S) sequence was obtained from one of the mermithid specimens. Phylogenetic analysis of this sequence and previously published nematode 18S sequences confirmed the morphological identification.

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Investigating hybridization in the parthenogenetic New Zealand stick insect Acanthoxyla (Phasmatodea) using single-copy nuclear loci

Cited by 29 publications

References 52 publications

Nonrandom Patterns of Genetic Admixture Expose the Complex Historical Hybrid Origin of Unisexual Leaf Beetle Species in the GenusCalligrapha

Nonrandom Patterns of Genetic Admixture Expose the Complex Historical Hybrid Origin of Unisexual Leaf Beetle Species in the GenusCalligrapha

A review of genetic analyses of hybridisation in New Zealand

First records of mermithid nematodes (Nematoda: Mermithidae) parasitising stick insects (Insecta: Phasmatodea)

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