Investigating non‐immune haemolytic transfusion reactions in surgical patients

Chand, Sreethu; Amita, R; Gupta, Debasish

doi:10.1111/tme.12914

Cited by 3 publications

(2 citation statements)

References 22 publications

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“…Timely detection of reaction to blood transfusion and immediate cessation of the transfusion, and also required support therapy are essential for a successful treatment of reactions to blood transfusion. If used correctly, transfusion of blood components can be a very beneficial form of low-risk therapy (Harrell & Kristensen, 1995;Brand, 2002;Sreethu et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

The content of leukocytes in the blood of recipient rabbits after allogeneic transfusion of packed red blood cells

Malyuk,

Yehorov,

Kharkevych

et al. 2024

Regul. Mech. Biosyst.

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Researching how the immune system reacts to stimuli such as blood transfusion of alloantigens on models of different species of laboratory animals, in particular rabbits, is important for understanding the mechanisms through which post-transfusion reactions develop. In this study, we identified the absolute and relative contents of white blood cells in blood of the recipient rabbits, and also their subpopulations after allogenic transfusion of packed red blood cells (pRBCs). Allogeneic transfusion of pRBCs without leukoreduction was conducted once, in the calculation of 5.5 mL/kg of body mass of the experimental animals. The material for the studies were samples of blood serum, collected from 5 rabbits on the 3rd, 7th, and 23rd days after pRBC administration. Allogeneic transfusion of pRBCs to the recipient rabbits caused development of leukocytosis in their blood: compared with the initial condition, the absolute content of leukocytes in the blood of the experimental animals increased 27.0% on the 3rd day after transfusion, equaling 8.94 109/L; 40.1% on the 7th day, accounting for 9.86 109/L; and 34.1% on the 23rd day, measuring 9.44 109/L. Leukocytosis resulted from increased content of granulocytes in the blood compared with the initial state: the absolute content of granulocytes surged 66.9% on the 3rd day after transfusion, measuring 4.54 109/L, 2.21-fold on the 7th day, equaling 6.02 109/L, and 1.87-fold on the 23rd day, equaling 5.08 109/L. Allogeneic transfusion of pRBCs to the recipient rabbits caused decrease in the relative content of Т-lymphocytes and increase in the content of В-lymphocytes in their blood. Compared with the initial condition, the relative content of Т-lymphocytes in the blood of the experimental animals declined 20.6% on the 3rd day of the study, accounting for 58.0%; 30.1% on the 7th day, equaling 51.0%; and 25.8% on the 23rd day, equaling 54.2%. Compared with the initial state, the relative content of В-lymphocytes spiked 94.7% on the 3rd day of the study, measuring 22.2%, 2.23-fold on the 7th day, accounting for 25.4%, and 2.51-fold on the 23rd day, equaling 28.6%.

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Section: Introductionmentioning

confidence: 99%

The content of leukocytes in the blood of recipient rabbits after allogeneic transfusion of packed red blood cells

Malyuk,

Yehorov,

Kharkevych

et al. 2024

Regul. Mech. Biosyst.

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“…Thus, it has been established that reactions to blood transfusion are immune and non-immune, which are mediated or not mediated by the immune system. They can appear either immediately after the transfusion of blood and its components, or some time after the transfusion (Harrell & Kristensen, 1995;Sreethu et al, 2022). Clinical signs in animals, particularly dogs, may include fever, redness, swelling in the muzzle, shortness of breath, which may resolve without specific treatment or complications (Davidow et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

The state of the humoral and cellular links of immunity of recipient rabbits following allogeneic transfusion of erythrocyte mass

Malyuk,

Yehorov,

Kharkevych

et al. 2023

Regul. Mech. Biosyst.

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The study of activation of the humoral link of immunity of recipient rabbits following allogeneic transfusion of erythrocyte mass is important for a fundamental understanding of the formation of immunity in model species of animals. We measured the contents of B-lymphocytes, immunoglobulins of M, G, A classes, circulating immune complexes in blood of the recipient rabbits and antibody-dependent cytotoxic activity of lymphocytes. Number of B-lymphocytes in the blood of the rabbits was determined according to Bianco. Immunoglobulin content in blood serum of the rabbits was measured based on the Mancini method. The spectrophotometry method was used to measure circulating immune complexes. The antibody-dependent cytotoxic activity of lymphocytes was identified by the colorimetric method. Modeling of erythrocyte mass transfusion was performed on five clinically healthy rabbits by intravenous injection of allogeneic erythrocyte mass in the dose of 5.5 mL/kg of body weight. The materials for the study were the blood samples, gathered on the 3rd, 7th, and 23rd days after transfusion. We found that the content of B-lymphocytes in blood of the recipient rabbits increased throughout the research: on the 3rd day the content of B lymphocytes, compared with the initial state (11.4%), increased by 1.94 times and was 22.2%; on the 7th day their content increased by 2.22 times and was 25.4%; and on the 23rd day of the experiment the content of lymphocytes increased by 2.46 times, compared with the initial state, equaling 28.6%. The content of IgM on the 3rd day after transfusion of erythrocyte mass increased by 54.9% and was 2.20 ± 0.70 g/L. On the 7th day it increased by 19.0%, equaling 1.69 ± 0.44 g/L, and on the 23rd day the content of immunoglobulins decreased by 54.2% compared with the initial state (1.42 ± 0.18 g/L, intact rabbits), accounting for 0.77 ± 0.25 g/L. The content of immunoglobulins of class G on the 3rd, 7th, and 23rd days of the experiment was nearly the same as in the initial state. The content of class A immunoglobulins decreased 4.16-fold on the 3rd day of the experiment, approaching the control values afterwards. After the transfusion of erythrocyte mass to the recipient rabbits, the content of circulating immune complexes was higher compared with the initial state throughout the research: on the 3rd day the CICs content increased by 2.13 times, measuring 6.40 conventional units, and on the 7th and 23rd days it approached the initial values of the control group. On the 3rd day after transfusion, the antibody-dependent cytotoxic activity of blood lymphocytes of erythrocyte mass of the rabbits increased compared with the initial state (15.9%), measuring 17.3%; on the 7th day it was 19.4%; and on the 23rd day it increased to 27.9%. The results revealed presence of characteristic immunological changes after allogeneic transfusion of erythrocyte mass to the recipient rabbits, which were a consequence of progressive increase in the activity of the humoral link of immunity.

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Spectrum of Hemolytic Transfusion Reactions from Tertiary Cancer Center Over a 1-year Period

Fathima,

Murugesan,

Anju

2023

Global Journal of Transfusion Medicine

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Background and Objectives: Acute hemolytic transfusion reactions (HTRs) one of the serious adverse events manifesting within 24 h of blood transfusion. To review the adverse transfusion reactions in our cancer center over a 1-year period, especially HTR and its causation to find the ways of mitigating the same. Methods: This was a retrospective analysis of HTR in tertiary care cancer center from South India. Patients who developed HTR in 2022 were included for discussion. Results: A total of six HTRs observed in five patients during 1 year. Case 1 was an acute presentation of HTR due to Anti Jka antibody. Case 2 was a nonimmune HTR associated with leukodepleted irradiated packed red blood cells transfusion. Case 3 was an incidental finding of passive hemolysis due to ABO incompatible platelet transfusion in patient clinically manifesting febrile and transfusion-associated dyspnea. Case 4 presented as an HTR with inconclusive evidence suggestive of HTR, with an impression of hemolysis as a part of the underlying disease. Conclusion: While the incidence of HTR due to ABO-incompatible transfusion reactions has been minimized, the other potential causes for acute HTR are still a concern. An effective hemovigiliance and strict adherence to standards in blood component practice may help to mitigate any form of transfusion reaction.

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Investigating non‐immune haemolytic transfusion reactions in surgical patients

Cited by 3 publications

References 22 publications

The content of leukocytes in the blood of recipient rabbits after allogeneic transfusion of packed red blood cells

The content of leukocytes in the blood of recipient rabbits after allogeneic transfusion of packed red blood cells

The state of the humoral and cellular links of immunity of recipient rabbits following allogeneic transfusion of erythrocyte mass

Spectrum of Hemolytic Transfusion Reactions from Tertiary Cancer Center Over a 1-year Period

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