Investigating promising substrates for promoting 1,4-dioxane biodegradation: effects of ethane and tetrahydrofuran on microbial consortia

Xiong, Yi; Mason, Olivia U.; Lowe, Ashlee; Zhang, Zhiming; Zhou, Chao; Chen, Gang; Villalonga, Michael Joseph; Tang, Youneng

doi:10.1007/s10532-020-09901-2

Cited by 21 publications

(12 citation statements)

References 64 publications

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“…TS43) from a stable 1,4-D-degrading microbial consortium ( Figure 1 and Figure 2 ). The Gram-positive bacteria belonging to the genus Pseudonocardia are well-known for their ability to degrade 1,4-D metabolically or co-metabolically and are commonly found in 1,4-D-degrading microbial consortia or mixed cultures enriched from different contaminated environments [ 1 , 35 , 36 , 37 ]. Until now, seven Pseudonocardia spp.…”

Section: Discussionmentioning

confidence: 99%

Isolation and Characterization of Novel Bacteria Capable of Degrading 1,4-Dioxane in the Presence of Diverse Co-Occurring Compounds

et al. 2021

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Biodegradation is found to be a promising, cost-effective and eco-friendly option for the treatment of industrial wastewater contaminated by 1,4-dioxane (1,4-D), a highly stable synthetic chemical and probable human carcinogen. This study aimed to isolate, identify, and characterize metabolic 1,4-D-degrading bacteria from a stable 1,4-D-degrading microbial consortium. Three bacterial strains (designated as strains TS28, TS32, and TS43) capable of degrading 1,4-D as a sole carbon and energy source were isolated and identified as Gram-positive Pseudonocardia sp. (TS28) and Gram-negative Dokdonella sp. (TS32) and Afipia sp. (TS43). This study, for the first time, confirmed that the genus Dokdonella is involved in the biodegradation of 1,4-D. The results reveal that all of the isolated strains possess inducible 1,4-D-degrading enzymes and also confirm the presence of a gene encoding tetrahydrofuran/dioxane monooxygenase (thmA/dxmA) belonging to group 5 soluble di-iron monooxygenases (SDIMOs) in both genomic and plasmid DNA of each of the strains, which is possibly responsible for the initial oxidation of 1,4-D. Moreover, the isolated strains showed a broad substrate range and are capable of degrading 1,4-D in the presence of additional substrates, including easy-to-degrade compounds, 1,4-D biodegradation intermediates, structural analogs, and co-contaminants of 1,4-D. This indicates the potential of the isolated strains, especially strain TS32, in removing 1,4-D from contaminated industrial wastewater containing additional organic load. Additionally, the results will help to improve our understanding of how multiple 1,4-D-degraders stably co-exist and interact in the consortium, relying on a single carbon source (1,4-D) in order to develop an efficient biological 1,4-D treatment system.

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Section: Discussionmentioning

confidence: 99%

Isolation and Characterization of Novel Bacteria Capable of Degrading 1,4-Dioxane in the Presence of Diverse Co-Occurring Compounds

et al. 2021

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“…Alkanes, such as propane and ethane, have emerged in the literature as potentially effective alternative cometabolite for 1,4-dioxane degradation. ,, Propane is an easily available commodity; it can be commercially delivered, and the price per gallon has remained below $3 for the last 10 years in the United States. The solubility of propane in water is 70 mg/L at 20 °C .…”

Section: Introductionmentioning

confidence: 99%

“…Recent studies have demonstrated that 1,4-dioxane can be biologically degraded, either as a sole carbon source − or cometabolically. − When 1,4-dioxane is degraded as the sole carbon source, typically high concentrations are needed to support cell metabolism. Theoretically, cometabolism is promising for addressing low concentrations of 1,4-dioxane because the cometabolite acts to sustain the cellular growth and enzyme production needed to drive degradation of 1,4-dioxane to low levels .…”

Section: Introductionmentioning

confidence: 99%

Cometabolic Treatment of 1,4-Dioxane in Biologically Active Carbon Filtration with Tetrahydrofuran and Propane at Relevant Concentrations for Potable Reuse

Stohr

Vaidya²,

Wilson

et al. 2023

ACS EST Water

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1,4-Dioxane is a probable human carcinogen and a widely occurring water contaminant. Cometabolic biodegradation of 1,4-dioxane could potentially provide a cost-effective and efficient treatment, but research is needed to establish its viability for water reuse purposes. The purpose of this study was to compare propane versus tetrahydrofuran as cometabolites for enhancing the removal of 1,4-dioxane via biologically active carbon filtration (BAF) as part of a pilot-scale water reuse system. Removal of 10, 5, 1, and 0.3 μg/L of 1,4-dioxane by BAF was examined in systems amended with either propane (2 mg/L) or tetrahydrofuran (25–50 μg/L), receiving the same ozonated, secondary wastewater effluent. At the lowest influent 1,4-dioxane concentration (0.3 μg/L), the tetrahydrofuran amended filter removed on average 58% ± 4% (std dev) of the incoming 1,4-dioxane. During the same period, the propane amended filter removed on average 74% ± 7% of the incoming 1,4-dioxane and at times degraded the 1,4-dioxane to below the method detection limit of 0.06 μg/L. This study demonstrates that amending cometabolites prior to BAF is a feasible treatment technique for removal of 1,4-dioxane occurring at submicrogram per liter concentrations and thus holds promise for both potable reuse applications and drinking water treatment.

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“…Previous studies revealed that a primary electron donor, such as ethane, propane, or tetrahydrofuran, promoted 1,4-dioxane degradation via co-metabolism in aerobic conditions. 15 However, adding exogenous organics is costly and potentially introduces secondary contamination. Furthermore, the coexisting chlorinated hydrocarbons (1,1,1-TCA, TCE, and 1,1dichloroethane (1,1-DCA)) can significantly inhibit the biodegradation of 1,4-dioxane.…”

Section: ■ Introductionmentioning

confidence: 99%

“…Although various microorganisms have been proven to metabolically or co-metabolically oxidize 1,4-dioxane, − the slow degradation rate (usually 0.1 to 2.0 mg of dioxane/h/mg of protein) hampers practical application of bioremediation. Previous studies revealed that a primary electron donor, such as ethane, propane, or tetrahydrofuran, promoted 1,4-dioxane degradation via co-metabolism in aerobic conditions . However, adding exogenous organics is costly and potentially introduces secondary contamination.…”

Section: Introductionmentioning

confidence: 99%

A Synergistic Platform for Continuous Co-removal of 1,1,1-Trichloroethane, Trichloroethene, and 1,4-Dioxane via Catalytic Dechlorination Followed by Biodegradation

Luo

Long

Wang

et al. 2021

Environ. Sci. Technol.

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Groundwater co-contaminated with 1,4-dioxane, 1,1,1-trichloroethane (TCA), and trichloroethene (TCE) is among the most urgent environmental concerns of the U.S. Department of Defense (DoD), U.S. Environmental Protection Agency (EPA), and industries related to chlorinated solvents. Inspired by the pressing need to remove all three contaminants at many sites, we tested a synergistic platform: catalytic reduction of 1,1,1-TCA and TCE to ethane in a H2-based membrane palladium-film reactor (H2-MPfR), followed by aerobic biodegradation of ethane and 1,4-dioxane in an O2-based membrane biofilm reactor (O2-MBfR). During 130 days of continuous operation, 1,1,1-TCA and TCE were 95–98% reductively dechlorinated to ethane in the H2-MPfR, and ethane served as the endogenous primary electron donor for promoting 98.5% aerobic biodegradation of 1,4-dioxane in the O2-MBfR. In addition, the small concentrations of the chlorinated intermediate from the H2-MPfR, dichloroethane (DCA) and monochloroethane (MCA), were fully biodegraded through aerobic biodegradation in the O2-MBfR. The biofilms in the O2-MBfR were enriched in phylotypes closely related to the genera Pseudonocardia known to biodegrade 1,4-dioxane.

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Investigating promising substrates for promoting 1,4-dioxane biodegradation: effects of ethane and tetrahydrofuran on microbial consortia

Cited by 21 publications

References 64 publications

Isolation and Characterization of Novel Bacteria Capable of Degrading 1,4-Dioxane in the Presence of Diverse Co-Occurring Compounds

Isolation and Characterization of Novel Bacteria Capable of Degrading 1,4-Dioxane in the Presence of Diverse Co-Occurring Compounds

Cometabolic Treatment of 1,4-Dioxane in Biologically Active Carbon Filtration with Tetrahydrofuran and Propane at Relevant Concentrations for Potable Reuse

A Synergistic Platform for Continuous Co-removal of 1,1,1-Trichloroethane, Trichloroethene, and 1,4-Dioxane via Catalytic Dechlorination Followed by Biodegradation

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