Investigating Protein-Nucleic Acid Binding Interactions with Diethylpyrocarbonate Covalent Labeling–Mass Spectrometry
Zachary J. Kirsch,
Jonathan Ashby,
Richard W. Vachet
Abstract:Nucleic acids are important biomolecules that facilitate numerous cellular functions and have in recent years become promising candidates for treating disease. Consequently, there is a need for methods to characterize protein interactions with these molecules. Here, we demonstrate that diethylpyrocarbonate (DEPC) covalent labeling−mass spectrometry (CL-MS) can provide structural information for protein-nucleic acid binding by characterizing the binding sites of two DNA aptamers specific to thrombin. Reductions… Show more
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