Investigating purification and activity analysis of urease enzyme extracted from jack bean source: A green chemistry approach

Pawar, Varsha S.; Bhande, Dhanashree; Pawar, Shivaji D.; Mudila, Harish; Kumar, Anil

doi:10.1016/j.ab.2022.114925

Cited by 9 publications

(2 citation statements)

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“…Plant and animals are the two primary sources used for the extraction of proteins. [ 1 ] Silkworms produce cocoon made of silk protein for morphogenesis from dormant pupae to an adult moth. Silks provide physical protection to silkworm from predators and acts as a temperature, humidity, and light sensor to provide insect with information about outside environment for successful metamorphosis.…”

Section: Introductionmentioning

confidence: 99%

Discovering Intrinsic Magnetism of Silk Fibroins

Ranade,

Choudhary,

Singh

2024

Macromolecular Symposia

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Silk fibroin (SF) is an interesting biomaterials with intriguing mechanical, optical, piezoelectric, and biocompatible properties. The present study reports the first magnetic experiments to show that organic radicals in the building blocks of SF induce permanent magnetization in these materials. A saturation magnetization of 2.8 × 10−3 emu g−1 and non‐zero remnant magnetization of 2.2 × 10−4 emu g−1 with a coercive field of 65 Oe is observed in multiple SFs. Tightly bound β‐sheets of polyglycine‐alanine motifs provides sufficient steric hinderance to organic radicals and protects them from attack of reactive species. Moreover, it also shows that silk's ferromagnetic character can be enhanced by simple mechanical stretching and heating. This work demonstrates a new paradigm of magnetic proteins and opens a route toward the biomimetic discovery of iron‐free magnetic proteins.

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Section: Introductionmentioning

confidence: 99%

Discovering Intrinsic Magnetism of Silk Fibroins

Ranade,

Choudhary,

Singh

2024

Macromolecular Symposia

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“…The higher the urease activity, the greater the potential for urea hydrolysis to occur. Specific urease activity is a measure of the activity of urease per unit of protein or biomass present in the sample [28]. Specific urease activity is a valuable parameter because it considers the influence of biomass or protein content on overall urease activity.…”

Section: Determination Of Urease Activitiesmentioning

confidence: 99%

Influence of Enriched Urease Producing Bacteria from Leachate and Restaurant Wastewater on Heavy Metal Removal

Basri,

Omoregie,

Mokhter

2023

Mal. J. Fund. Appl. Sci.

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The escalation of heavy metal pollution in natural ecosystems due to industrialization presents a critical environmental concern, endangering the well-being of living organisms. Microbially Induced Carbonate Precipitation (MICP) technology, an emerging innovation, has gained attention from the scientific community for its potential in biocementation and bioremediation applications. However, a substantial gap in understanding exists regarding the utilization of ureolytic microbial strains from waste sources capable of effectively immobilizing high concentrations of heavy metals. This study endeavors to explore the latent potential of indigenous ureolytic bacteria derived from leachate and restaurant wastewater, possessing bioremediation capabilities for heavy metal immobilization. The investigation includes microbial screening, physiological characterization of ureolytic bacteria, assessment of their tolerance levels, and evaluation of heavy metal removal efficacy through Atomic Absorption Spectrophotometry (AAS) analysis. Notably, the results reveal that ureolytic bacteria from restaurant wastewater are more tolerant to Cd2+ concentrations compared to their leachate counterparts, manifesting optimum conductivity, pH, and optical density (OD). More so, AAS analysis demonstrates the restaurant wastewater-derived sample's remarkable proficiency in Cd2+ removal, achieving a substantial 95% removal rate, significantly outperforming the leachate wastewater sample's removal rate of 53%.

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