Investigating role of Triton X-100 in ameliorating deleterious effects of anthracene in wheat plants

Abstract

This study focused on the deleterious effect of anthracene (ANT) and role of a surfactant, Triton (TX-100), in recovery from inhibitory effect of ANT. Fast chlorophyll (Chl) fluorescence measurements were performed in wheat plants. Results revealed that maximum quantum yield of PSII, area over the fluorescence curve, performance index (PI), and reaction centre density was negatively affected by ANT treatment. The effects on PSII quantum efficiency, reaction centre density, absorption, and trapping were partially recovered by TX-100. PSII heterogeneity in terms of PSII antenna heterogeneity, corresponding to PSII α, β, and γ centres, and reducing side, corresponding to Q B -reducing and Q B -nonreducing centres, were also investigated. The damage caused by ANT to PSII antenna heterogeneity was recovered almost by 100% owing to TX-100.

Effect of Triton X-100 on the wheat and lettuce growth and contaminant absorption

“…6. The chlorophyll-a concentrations can be used to indicate the general health status of the plant since photosynthesis is one of the important processes [23]. The average chlorophyll-a concentrations increased with increasing Triton X-100 concentrations from 0 (i.e., 1.06 mg L −1 ) to 240 mg L −1 (2.15 mg L −1 ).…”

Effects of Burkholderia thailandensis rhamnolipids on the unicellular algae Dunaliella tertiolecta

“…Based on the latter, the fluctuation of Chl/cell and carotenoids/cell currently observed in control cells can be attributed to acclimation of the algae in the new culture medium after inoculation, while the light or nutrient starvation seemed to cause negligible effects, at least under such a short-term culture treatment (0-96 h). On the other hand, it is therefore most likely that RLs counteracted any temporal environmental pressure through modification of membrane permeability, since it is known that smalls changes in RLs-treated algal surface tension could lead to slight alterations of membrane permeability, preserving or even stimulating pigment formation (Sharma et al, 2018), which in turn could stimulate the growth of cell culture (Lowe et al 1994).…”

“…Consequently, the sensitivity of this technique is expected to be high, as the impact site of a tested substance could be related to any of those steps but not to the total process. To our knowledge, despite the fact that chlorophyll fluorescence has been widely used for two decades in ecotoxicology studies (Ralph et al, 2007;Kumar et al, and references there-in), the JIP-test has been sporadically used the last years (Appenroth et al, 2001;Geoffroy et al, 2003;Xia and Tian, 2009) and only in one study regarding impact of a chemical surfactant on wheat plants (Sharma et al, 2018).…”