Investigating ROS sources in male infertility: A common end for numerous pathways

Lavranos, Giagkos; Balla, Murali Mohan Sagar; Tzortzopoulou, A.; Syriou, Vassiliki; Angelopoulou, Roxani

doi:10.1016/j.reprotox.2012.06.007

Cited by 143 publications

(93 citation statements)

References 116 publications

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“…Whereas the role of ROS in the reproductive male function has been widely studied [33][34][35][36][37], only in last few years attention of researchers has been also focused on the female reproductive function. It is believed that ROS over-production in the reproductive tract may significantly contribute to woman infertility mainly because of the ability of these molecules to exert a toxic effect decreasing oocyte quality [2,38,39].…”

Section: Discussionmentioning

confidence: 99%

Protein modification as oxidative stress marker in follicular fluid from women with polycystic ovary syndrome: the effect of inositol and metformin

Piomboni

Focarelli

Capaldo

et al. 2014

J Assist Reprod Genet

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Purpose The purpose of this study was to evaluate the oxidative stress status (OS) of follicular fluid (FF) and the oocyte quality in women with polycystic ovary syndrome (PCOS) undergoing different ovarian stimulation protocols. Methods FF samples were collected after gonadotropin administration in association or not with metformin or D-chiro-inositol (DCI). OS status was then evaluated by checking the follicular fluid protein oxidation profile after specific labeling of aminoacidic free-SH groups, and two-dimensional electrophoresis followed by qualitative and semiquantitative analysis. Oocyte quality was assessed by international morphological criteria. Results Our data indicated that both treatments, even if to different extent, recovered a significantly high level of free-SH groups in FF proteins of PCOS women clearly indicating a decrease of OS level with respect to that found in FF samples from gonadotropins alone treated women. A higher number of good quality MII oocytes was also observed in DCI (P<0.05) or metformin (P<0.05) study groups in comparison to untreated control group. Conclusion A natural supplement and a drug both showed a statistically significant positive effect on follicular milieu by decreasing the oxidative damage on FF proteins, as well as in recovering good quality oocytes.

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Section: Discussionmentioning

confidence: 99%

Protein modification as oxidative stress marker in follicular fluid from women with polycystic ovary syndrome: the effect of inositol and metformin

Piomboni

Focarelli

Capaldo

et al. 2014

J Assist Reprod Genet

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“…ROS are significant mediators in signal transduction mechanisms and have function in sperm capacitation and acrosome reaction, and in regulation of spermatozoa and oocyte joining [68][69][70]. In addition, biological agents, radiation, excess heat, some medications, radiation, exposure to heavy metals, and smoking lead to increased ROS production in the testes and epididymis [71]. Human spermatozoal membrane is comprised of a high quantity of polyunsaturated fatty acids, which are the primary targets of oxidative stress.…”

Section: Free Radical Theory Of Aging and Apoptosismentioning

confidence: 99%

“…Human spermatozoal membrane is comprised of a high quantity of polyunsaturated fatty acids, which are the primary targets of oxidative stress. After spermatozoal membrane, other targets of oxidative stress include biomolecules, such as structural proteins and nucleic acids [52,71]. Spermatozoa contain a small amount of cytoplasm, and therefore are poor in antioxidant enzymes like superoxide dismutase, glutathione peroxidase, and catalase.…”

Section: Free Radical Theory Of Aging and Apoptosismentioning

confidence: 99%

Effects of aging on the male reproductive system

Güneş

Hekim

Arslan

et al. 2016

J Assist Reprod Genet

195

118

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The study aims to discuss the effects of aging on the male reproductive system. A systematic review was performed using PubMed from 1980 to 2014. Aging is a natural process comprising of irreversible changes due to a myriad of endogenous and environmental factors at the level of all organs and systems. In modern life, as more couples choose to postpone having a child due to various socioeconomic reasons, research for understanding the effects of aging on the reproductive system has gained an increased importance. Paternal aging also causes genetic and epigenetic changes in spermatozoa, which impair male reproductive functions through their adverse effects on sperm quality and count as, well as, on sexual organs and the hypothalamic-pituitarygonadal axis. Hormone production, spermatogenesis, and testes undergo changes as a man ages. These small changes lead to decrease in both the quality and quantity of spermatozoa. The offspring of older fathers show high prevalence of genetic abnormalities, childhood cancers, and several neuropsychiatric disorders. In addition, the latest advances in assisted reproductive techniques give older men a chance to have a child even with poor semen parameters. Further studies should investigate the onset of gonadal senesce and its effects on aging men.

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“…In humans, physiological ROS is beneficial for the cyclic waves of spermatogenesis, whereas excess ROS are harmful and can cause human infertility (51)(52)(53). Our present study indicated that aberrant elevation of ROS caused by suppression of STIM1 in Sertoli cells in early mouse gonads could impair testicular cord formation.…”

Section: Discussionmentioning

confidence: 59%

“…As shown in supplemental Fig. S1, the largest proportion of DE proteins identified was annotated as cytoplasm (191), followed by membrane (138), nucleus (85), and mitochondrion (52).…”

Section: Bioinformatics Analysis Of De Proteins Reveals Possible Impomentioning

confidence: 99%

Quantitative Proteomics Reveals the Essential Roles of Stromal Interaction Molecule 1 (STIM1) in the Testicular Cord Formation in Mouse Testis

Zheng

Zhao

Zhang

et al. 2015

Molecular & Cellular Proteomics

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Testicular cord formation in male gonadogenesis involves assembly of several cell types, the precise molecular mechanism is still not well known. With the high-throughput quantitative proteomics technology, a comparative proteomic profile of mouse embryonic male gonads were analyzed at three time points (11.5, 12.5, and 13.5 days post coitum), corresponding to critical stages of testicular cord formation in gonadal development. 4070 proteins were identified, and 338 were differentially expressed, of which the Sertoli cell specific genes were significant enrichment, with mainly increased expression across testis cord development. Additionally, we found overrepresentation of proteins related to oxidative stress in these Sertoli cell specific genes. Of these differentially expressed oxidative stress-associated Sertoli cell specific protein, stromal interaction molecule 1, was found to have discrepant mRNA and protein regulations, with increased protein expression but decreased mRNA levels during testis cord development. Knockdown of Stim1 in Sertoli cells caused extensive defects in gonadal development, including testicular cord disruption, loss of interstitium, and failed angiogenesis, together with increased levels of reactive oxygen species. And suppressing the aberrant elevation of reactive oxygen species could partly rescue the defects of testicular cord development. Taken together, our results suggest that reactive oxygen species regulation in Sertoli cells is important for gonadogenesis, and the quantitative proteomic data could be a rich resource to the elucidation of regulation of testicular cord development. Molecular & Cellular

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Investigating ROS sources in male infertility: A common end for numerous pathways

Cited by 143 publications

References 116 publications

Protein modification as oxidative stress marker in follicular fluid from women with polycystic ovary syndrome: the effect of inositol and metformin

Protein modification as oxidative stress marker in follicular fluid from women with polycystic ovary syndrome: the effect of inositol and metformin

Effects of aging on the male reproductive system

Quantitative Proteomics Reveals the Essential Roles of Stromal Interaction Molecule 1 (STIM1) in the Testicular Cord Formation in Mouse Testis

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