Investigating the association between the urinary microbiome and bladder cancer: An exploratory study

Hussein, Ahmed A.; Elsayed, Ahmed S.; Durrani, Mohammad; Jing, Zhe; Iqbal, Umar; Gomez, Eduardo Cortes; Singh, Prashant; Liu, Song; Smith, Gary J.; Tang, Li

doi:10.1016/j.urolonc.2020.12.011

Cited by 55 publications

(87 citation statements)

References 31 publications

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“…In this study, urine microbial diversity was greater in healthy dogs as compared to dogs with UC, a finding that aligns with several studies on urine microbiota in humans with UC [37,39]. However, there are also studies in humans that report no differences in microbial diversity or decreased diversity in urine from healthy individuals as compared to those with UC [17,35,36,38,42,44,79]. Differences in microbial composition (Unweighted UniFrac) have also been reported in previous human studies on UC [36,38,43,44].…”

Section: Urine and Fecal Microbiota Associated With Ucsupporting

confidence: 88%

“…These phyla also dominate the urine microbiota in humans [17,36,38,40,44,45] and have been reported in previous studies on healthy dog urine [80,81]. In humans, taxa associated with UC vary widely across studies, but Acinetobacter and Actinomyces have been found at increased abundances in patients with UC across at least three studies [35,42,44]. In this study, we did not see Acinetobacter or Actinomyces spp.…”

Section: Urine and Fecal Microbiota Associated With Ucsupporting

confidence: 81%

“…In this study, the four most abundant phyla in urine were Firmicutes, Actinobacteria, Bacteroides, and Proteobacteria. These phyla also dominate the urine microbiota in humans [17,36,38,40,44,45] and have been reported in previous studies on healthy dog urine [80,81]. In humans, taxa associated with UC vary widely across studies, but Acinetobacter and Actinomyces have been found at increased abundances in patients with UC across at least three studies [35,42,44].…”

Section: Urine and Fecal Microbiota Associated With Ucsupporting

confidence: 65%

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Urine and Fecal Microbiota in a Canine Model of Bladder Cancer

Mrofchak

Madden

Evans

et al. 2021

Preprint

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Introduction: Urothelial carcinoma (UC) is the tenth most diagnosed cancer in humans worldwide. Dogs are a robust model for invasive UC as tumor development and progression is similar in humans and dogs. Recent studies on urine microbiota in humans revealed alterations in microbial diversity and composition in individuals with UC; however, the potential role of microbiota in UC has yet to be elucidated. Dogs could be valuable models for this research, but microbial alterations in dogs with UC have not been evaluated. Objective: The objective of this this pilot study was to compare the urine and fecal microbiota of dogs with UC (n = 7) and age-, sex-, and breed-matched healthy controls (n = 7). Methods: DNA was extracted from mid-stream free-catch urine and fecal samples using Qiagen Bacteremia and PowerFecal kits, respectively. 16S rRNA gene sequencing was performed followed by sequence processing and analyses (QIIME 2 and R). Results: Canine urine and fecal samples were dominated by taxa similar to those found in humans. Significantly decreased microbial diversity (Kruskal-Wallis: Shannon, p = 0.048) and altered bacterial composition were observed in the urine but not feces of dogs with UC (PERMANOVA: Unweighted UniFrac, p = 0.011). The relative abundances of Fusobacterium was also increased, although not significantly, in the urine and feces of dogs with UC. Conclusion: This study characterizes urine and fecal microbiota in dogs with UC, and it provides a foundation for future work exploring host-microbe dynamics in UC carcinogenesis, prognosis, and treatment.

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Section: Urine and Fecal Microbiota Associated With Ucsupporting

confidence: 88%

Section: Urine and Fecal Microbiota Associated With Ucsupporting

confidence: 81%

Section: Urine and Fecal Microbiota Associated With Ucsupporting

confidence: 65%

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Urine and Fecal Microbiota in a Canine Model of Bladder Cancer

Mrofchak

Madden

Evans

et al. 2021

Preprint

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“…These findings support our hypothesis. In contrast, several investigators have suggested that significant biomarkers in NMIBC differ from those in MIBC (34)(35)(36). Unfortunately, our study design provides little definitive information to conclude about this issue; however, our results are important in proposing the clinical usefulness of urine CP as a predictive biomarker in patients with BC.…”

Section: Discussionmentioning

confidence: 62%

Ceruloplasmin Levels in Cancer Tissues and Urine Are Significant Biomarkers of Pathological Features and Outcome in Bladder Cancer

et al. 2021

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Background/Aim: Α previous report showed that immune complex-ceruloplasmin (CP) in urine is associated with carcinogenesis and malignant behavior in bladder cancer (BC). We investigated the pathological significance and prognostic roles of urine and tissue levels of CP protein in BC patients. Materials and Methods: Urine CP levels were measured using an enzyme-linked immunosorbent assay in 97 patients. CP expression in BC tissues was evaluated by immunohistochemical analysis in 176 patient samples. Results: Urine CP levels were positively associated with tumor grade and pT stage in non-muscle invasive BC (NMIBC). CP expression in BC tissues was positively associated with tumor growth and progression. Multivariate analysis demonstrated that high urine CP levels was an independent predictor of recurrence in the urinary tract in NMIBC (hazard ratio=2.87, p=0.016). Conclusion: CPrelated markers, especially urine CP levels, are useful biomarkers of malignant potential and prognosis in NMIBC.

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“…We compared the list of microbes obtained from the TCGA database with microbes that have been discovered in bladder cancer samples through 16s rRNA sequencing. A recent study investigated the bladder cancer microbiome using samples from 43 bladder cancer patients and 10 controls through 16s sequencing [31]. This study found the Actinomyces, Achromobacter, Brevibacterium, and Brucella genera to be highly differentially abundant in bladder cancer patients.…”

Section: Discussionmentioning

confidence: 99%

The Bladder Microbiome Is Associated with Epithelial–Mesenchymal Transition in Muscle Invasive Urothelial Bladder Carcinoma

Iyangar

Reddy

et al. 2021

Cancers

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The intra-tumor microbiome has recently been linked to epithelial–mesenchymal transition (EMT) in a number of cancers. However, the relationship between EMT and microbes in bladder cancer has not been explored. In this study, we profiled the abundance of individual microbe species in the tumor samples of over 400 muscle invasive bladder carcinoma (MIBC) patients. We then correlated microbe abundance to the expression of EMT-associated genes and genes in the extracellular matrix (ECM), which are key players in EMT. We discovered that a variety of microbes, including E. coli, butyrate-producing bacterium SM4/1, and a species of Oscillatoria, were associated with expression of classical EMT-associated genes, including E-cadherin, vimentin, SNAI2, SNAI3, and TWIST1. We also found significant correlations between microbial abundance and the expression of genes in the ECM, specifically collagens and elastin. Lastly, we found that a large number of microbes exhibiting significant correlations to EMT are also associated with clinical prognosis and outcomes. We further determined that the microbes we profiled were likely not environmental contaminants. In conclusion, we discovered that the intra-tumoral microbiome could potentially play a significant role in the regulation of EMT in MIBC.

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Investigating the association between the urinary microbiome and bladder cancer: An exploratory study

Cited by 55 publications

References 31 publications

Urine and Fecal Microbiota in a Canine Model of Bladder Cancer

Urine and Fecal Microbiota in a Canine Model of Bladder Cancer

Ceruloplasmin Levels in Cancer Tissues and Urine Are Significant Biomarkers of Pathological Features and Outcome in Bladder Cancer

The Bladder Microbiome Is Associated with Epithelial–Mesenchymal Transition in Muscle Invasive Urothelial Bladder Carcinoma

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