Investigating the biological response of human mesenchymal stem cells to titanium surfaces

Abstract

BackgroundWe have investigated the behaviour of a newly characterised population of haemarthrosis fluid-derived human mesenchymal stem cells (HF-hMSCs) with titanium (Ti) surfaces.MethodsHF-hMSCs were seeded onto round cannulated interference (RCI; Smith and Nephew) screws or control Ti discs and cultured under pro-osteogenic conditions.ResultsElectron microscopy showed the attachment and spreading of HF-hMSCs across both Ti surfaces during the early stages of osteogenic culture; however, cells were exclusively localised to the basal regions within the vertex of the Ti screws. In the later stages of culture, an osteoid matrix was deposited on the Ti surfaces with progressive culture expansion and matrix deposition up the sides and the top of the Ti Screws. Quantification of cellular content revealed a significantly higher number of cells within the Ti screw cultures; however, there was no difference in the cellular health. Conversely, alizarin red staining used as both a qualitative and quantitative measure of matrix calcification was significantly increased in Ti disc cultures compared to those of Ti screws.ConclusionsOur results suggest that the gross topography of the metal implant is able to create microenvironment niches that have an influence on cellular behaviour. These results have implications for the design of advanced tissue engineering strategies that seek to use cellular material to enhance biological remodelling and healing following tissue reconstruction.

Engineered coatings for titanium implants to present ultra-low doses of BMP-7

“…Ti chemistry favors the formation of a biocompatible titanium oxide surface layer, which also imparts increased resistance to corrosion. Therefore, Ti is often regarded as a more advantageous implant material, when compared with other metals such as cobalt, nickel, chromium, and their respective alloys. , The rate of bone formation, the implant surface area directly contacting bone, and the mechanical properties of the bone/implant interface are influenced by the nature of the implant surface . Previous studies to enhance the surface of Ti as an implant material were challenged by confounding factors .…”

Nanostructured Modifications of Titanium Surfaces Improve Vascular Regenerative Properties of Exosomes Derived from Mesenchymal Stem Cells: Preliminary In Vitro Results

“…Sequence FOR (5 -3 ) Sequence REV (5 -3 ) bFGF 1 TCCATCCTTTCTCCCTCGTTTC TCAGTAGATGTTTCCCTCCAATGTTTC BMP2 2 CCACTAATCATGCCATTGTTCAGAC CTGTACTAGCGACACCCACAA HGF 3 CTTCAATAGCATGTCAAGTGGAGTG GCTGCGTCCTTTACCAATGATG TFRC 4 TGTTTGTCATAGGGCAGTTGGAA ACACCCGAACCAGGAATCTC TGFB1 5 TGACAGCAGGGATAACACACT CCGTGGAGCTGAAGCA VEGFA 6 GGACAGAAAGACAGATCACAGGTAC GCAGGTGAGAGTAAGCGAAGG…”

“…CD31 1 TGAGACCAGCCTGATGAAACC CGATCTCCGCTCACTACAACC TFRC 2 TGTTTGTCATAGGGCAGTTGGAA ACACCCGAACCAGGAATCTC VEGFR2 3 GGAGGAGGAGGAAGTATGTGACC AACCATACCACTGTCCGTCTG VWF 4 ACGTATGGTCTGTGTGGGATC GACAAGACACTGCTCCTCCA…”

“…Titanium-based scaffolds are routinely used in both orthopedics and the cardiovascular field. Ti alloys are some of the most commonly used materials for clinical implantation due to their superior mechanical strength, corrosion resistance, and biocompatibility [2,3]. More importantly, the surfaces of Ti alloys can be easily modified to have micro-and nanolevels of organization to mimic the natural structure of healthy vessel walls, making them more cytocompatible [4].…”