Investigating the effect of GDF9 , BMP15 , BMP6 and BMPR1B polymorphisms on Egyptian sheep fecundity and their transcripts expression in ovarian cells

El-Halawany, Nermin; Kandil, Omaima M.; Shawky, Abd-El-Monsif A.; Al-Tohamy, Ahmed F.M.; El-Sayd, Yasmeen A.; Abdel-Shafy, Hamdy; Abou-Fandoud, E. I.; Abdel-Azeem, Safaa N.; El-Rahim, Abeer H. Abd; Abdoon, Ahmed S.; Michal, Jennifer J.; Jiang, Zhihua

doi:10.1016/j.smallrumres.2018.06.010

Cited by 12 publications

(5 citation statements)

References 51 publications

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“…Similarly, with our transcriptomic analysis in the Pelibuey sheep, we have identified genes that are associated with multiple calving sheep and have been previously reported in other sheep breeds such as the IL1A (Interleukin 1 α) and CYP2S1 (Metabolism of xenobiotics by Cytochrome P450) genes in Hu ewes, the function of these genes in the process of folliculogenesis is unknown [14]; the BMP15 and INHBA (Inhibin β A subunit) genes in Finnsheep ewes [26]. The BMP15 gene has activity in the oocytes (both immature and mature) within the ovary, stimulating the proliferation of GC in a follicle-stimulating hormone receptor (FSHR)-independent manner [2] and INHBA gene shows a considered level of mRNA expression in the GC of antral follicles during the stages of the estrous cycle in sheep [27]. While the gene IL1A has activity in the mechanism of apoptosis, which is responsible for the elimination of oocytes and a deficiency or blockade of increased expression of FSHR in GC in rodents [26].…”

Section: Discussionmentioning

confidence: 99%

“…It is directly related to traits such as ovulation rate and litter size, which are genetically affected by causative mutations in some minor and major genes [1] and their interactions with the environment. The litter size improvement is one of the most important objectives for sheep breeders as it greatly increases production efficiency and economic gains [2], and therefore its analysis with molecular tools is of great importance for the profitability of sheep production. The Pelibuey sheep ( Ovis aries ) is an indigenous breed, distributed in the tropical regions of Mexico with adaptability to climatic variations, maternal capacity with a low reproductive seasonality that allows productivity throughout the year and an average litter size from 1–1.5 [3,4].…”

Section: Introductionmentioning

confidence: 99%

“…In recent years, a group of genes with different mutations (SNPs) has been identified and is associated with prolificacy in different wool sheep breeds. Within this group is the family of the transforming growth factor β (TGF-β) genes, where the SNPs result in an increased ovulation rate (OR) in sheep [2,5]. The bone morphogenetic protein receptor 1B ( BMPR1B ) gene, and their SNP identified as FecB B , are associated with hyperprolific phenotypes of Booroola ewes [6].…”

Section: Introductionmentioning

confidence: 99%

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RNA-seq Transcriptome Analysis in Ovarian Tissue of Pelibuey Breed to Explore the Regulation of Prolificacy

Hernández-Montiel

Collí-Dulá

Ramón-Ugalde

et al. 2019

Genes

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The Pelibuey sheep (Ovis aries) is an indigenous breed distributed in the tropical regions of Mexico. The prolificacy of this sheep is on average from 1 to 1.5 lambs, being an important breeding characteristic that owners seek to increase with the purpose of economic improvements. New-generation RNA sequencing technology has been used to identify the genes that are expressed in the ovarian tissue of sheep that have two or more lambs per parturition, as well as to elucidate the metabolic pathways that are affected by the expression of these genes, with the purpose of better understanding the prolificacy in the sheep. In the present study, the transcriptional expression of multiparous and uniparous sheep was compared using RNA sequencing. Multiparous (M group) and uniparous (U group) sheep that had a genealogical record for three generations (M, n = 5 and U, n = 5) were selected. RNA was extracted from ovarian tissue and subsequently used to prepare the libraries that were sequenced using the Illumina NextSeq500 platform. A total of 31,575 genes were detected from the transcriptomic analysis of which 4908 were significantly expressed (p-value ≤ 0.001) in the ovary of sheep. Subsequently, a second filter was carried out to evaluate the false discovery rate (FDR) and select those genes with p-values ≤ 0.05 and values of expression ≥ 1 (log2), obtaining 354 differential expressed genes (DEG): 120 genes up-regulated and 234 genes down-regulated in the group M with respect to the group U. Through Gene Ontology (GO) and metabolic analysis, we obtained information on the function of differentially expressed genes, and its importance in the reproduction of multiparous sheep. This result suggest that genes identified in the present study participate in the development of the final stages of follicles.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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RNA-seq Transcriptome Analysis in Ovarian Tissue of Pelibuey Breed to Explore the Regulation of Prolificacy

Hernández-Montiel

Collí-Dulá

Ramón-Ugalde

et al. 2019

Genes

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“…Reproductive traits, such as prolificacy, multiple births, and fecundity, are threshold and crucial for the profitability of sheep production but have low heritability rate [1][2][3]. Therefore, developing reproductive traits has become a significant interest in sheep breeding [4], as it can increase the production of sheep-derived products, ranging from wool to lamb meat [5].…”

Section: Introductionmentioning

confidence: 99%

Genome wide identification and characterization of fertility associated novel CircRNAs as ceRNA reveal their regulatory roles in sheep fecundity

et al. 2023

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Reproductive traits play a vital role in determining the production efficiency of sheep. Maximizing the production is of paramount importance for breeders worldwide due to the growing population. Circular RNAs (circRNAs) act as miRNA sponges by absorbing miRNA activity through miRNA response elements (MREs) and participate in ceRNA regulatory networks (ceRNETs) to regulate mRNA expression. Despite of extensive research on role of circRNAs as miRNA sponges in various species, their specific regulatory roles and mechanism in sheep ovarian tissue are still not well understood. In this study, we performed whole genome sequencing of circRNAs, miRNA and mRNA employing bioinformatic techniques on ovine tissues of two contrasting sheep breeds "Small tail Han (X_LC) and Dolang sheep (D_LC)", which results into identification of 9,878 circRNAs with a total length of 23,522,667 nt and an average length of 2,381.32 nt. Among them, 44 differentially expressed circRNAs (DECs) were identified. Moreover, correlation between miRNA-mRNA and lncRNA-miRNA provided us with to prediction of miRNA binding sites on nine differentially expressed circRNAs and 165 differentially expressed mRNAs using miRanda. miRNA-mRNA and lncRNA-miRNA pairs with negative correlation were selected to determine the ceRNA score along with positively correlated pairs from lncRNA and mRNA network. Integration of ceRNA score and positively correlated pairs exhibit a significant ternary relationship among circRNAs-miRNA-mRNA demonestrated by ceRNA, comprising of 50 regulatory pairs sharring common nodes and predicted potential differentially expressed circRNAs-miRNAs-mRNAs regulatory axis. Based on functional enrichment analysis shortlisted key ceRNA regulatory pairs associated with reproduction including circRNA_3257-novel579_mature-EPHA3, circRNA_8396-novel130_mature-LOC101102473, circRNA_4140- novel34_mature > novel661_mature-KCNK9, and circRNA_8312-novel339_mature-LOC101110545. Furthermore, expression profiling, functional enrichments and qRT-PCR analysis of key target genes infer their implication in reproduction and metabolism. ceRNA target mRNAs evolutionary trajectories, expression profiling, functional enrichments, subcellular localizations following genomic organizations will provide new insights underlying molecular mechanisms of reproduction, and establish a solid foundation for future research. Graphical Abstract Graphical abstract summarizing the scheme of study

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“…The bone morphological receptor gene BMPR1B is considered one of the fertility genes responsible for the reproduction of ewes and has a major role in the growth and reproduction of ovarian granulosa cells in sheep [17][18][19][20][21]. It belongs to type I receptor [22] BMPs, which are prominently seen in proliferating primordial germ cells [23]. Piper and Bindon [ 15 ] reported that the fertility of sheep breeds may result in part from the action of a single master gene affecting ovulation rate.…”

Section: Discussionmentioning

confidence: 99%

Genetic Variation of rs429976637, rs416717560, rs403555643 and rs427046713 in BMPRP1B Gene and its Frequency of Awassi Sheep

Essa,

Mohammed,

Al-Anbari

2023

IOP Conf. Ser.: Earth Environ. Sci.

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Bone protein receptor gene (BMPR1B) is a significant fertility gene found in sheep globally and associated to reproductive and fertility features. This study verified the relationship between the BMPR1B gene’s 893 G A SNP1, SNP2, SNP3, and SNP4 C T 893. 35 Awassi ewe blood samples were acquired from the Agricultural Research Department in Anbar Governorate – Al Dawar, followed by DNA extraction to validate the BMPR1B gene and PCR amplification. The Sanger Sequences technique of single nucleotide polymorphism (SNP) was employed to determine each ewe’s special alleles using the gene primer. Four BMPR1B gene sites had substantially varied genotype and allele frequencies (P < 0.01). The association study indicated that the rs429976637 (G>A) locus had three polymorphisms, GG, GA, and AA, with percentages of 92.16, 7.68, and 0.16%, respectively, and allelic frequencies of G - 0.96 and A-0.04. Polymorphisms differed (P=0.01). rs416717560 (G>A) had three polymorphisms: GG, GA, and AA. The allelic frequency of the G alleles was 0.91, and polymorphism was 82.82, 16.38, and 0.80%. Polymorphism differed by 0.09 (P=0.01). The rs403555643 locus (A>G) has three polymorphisms, AA, AG, and GG, with percentages of 92.14, 7.68, and 0.16%, respectively, and allelic frequencies of A-0.96 and G - 0.04 (P < 0.01). The locus rs427046713 (C>T) had three polymorphisms, CC, CT, and TT, with percentages of 94.09, 5.82, and 0.09%, respectively. The allelic frequency of C alleles was 0.97- and T 0.03--P < 0.01. The allelic frequency of the BMPR1B gene for four loci is considerably greater for genotypes GG, GG, AA, and CC.

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Investigating the effect of GDF9 , BMP15 , BMP6 and BMPR1B polymorphisms on Egyptian sheep fecundity and their transcripts expression in ovarian cells

Cited by 12 publications

References 51 publications

RNA-seq Transcriptome Analysis in Ovarian Tissue of Pelibuey Breed to Explore the Regulation of Prolificacy

RNA-seq Transcriptome Analysis in Ovarian Tissue of Pelibuey Breed to Explore the Regulation of Prolificacy

Genome wide identification and characterization of fertility associated novel CircRNAs as ceRNA reveal their regulatory roles in sheep fecundity

Genetic Variation of rs429976637, rs416717560, rs403555643 and rs427046713 in BMPRP1B Gene and its Frequency of Awassi Sheep

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