Investigating the Effect of Polymeric Approaches on Circulation Time and Physical Properties of Nanobubbles

Toit, Lisa C. du; Govender, Thirumala; Pillay, Viness; Choonara, Yahya E.; Kodama, Tetsuya

doi:10.1007/s11095-010-0247-y

Cited by 32 publications

(25 citation statements)

References 24 publications

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“…The NMBs used in the present study lacked stability in vivo, and the encapsulated C 3 F 8 gas was readily released and dispersed into the fluid around the NMBs (du Toit et al 2011;Sax and Kodama 2013). In previous experiments in which NMBs were circulated through blood vessels Li et al 2013a), echogenicity in the proper-ALN was found to decrease by 50%, 2 min after intravenous injection; this is consistent with the observations illustrated in Figure 3Bb.…”

Section: Discussionsupporting

confidence: 90%

Delivery of Molecules to the Lymph Node via Lymphatic Vessels Using Ultrasound and Nano/Microbubbles

Kato¹,

Shirai²,

Kanzaki³

et al. 2015

Ultrasound in Medicine & Biology

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Section: Discussionsupporting

confidence: 90%

Delivery of Molecules to the Lymph Node via Lymphatic Vessels Using Ultrasound and Nano/Microbubbles

Kato¹,

Shirai²,

Kanzaki³

et al. 2015

Ultrasound in Medicine & Biology

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“…2,10 More recently, submicron-sized bubbles, termed nanobubbles, have been designed and proposed as a prospective tool for gene delivery. [11][12][13][14] An advantage of nanosized bubbles is their potential extravasation capacity and their potential to accumulate in pathological tissues via the enhanced permeability and retention effect. 15,16 In a previous study, we developed DNA-loaded diethylaminoethyl (DEAE)-dextran nanobubbles as gene delivery systems.…”

Section: Introductionmentioning

confidence: 99%

New chitosan nanobubbles for ultrasound-mediated gene delivery: preparation and in vitro characterization

Cavalli¹,

Bisazza²,

Trotta³

et al. 2012

IJN

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Background:The development of nonviral gene delivery systems is one of the most intriguing topics in nanomedicine. However, despite the advances made in recent years, several key issues remain unsettled. One of the main problems relates to the difficulty in designing nanodevices for targeted delivery of genes and other drugs to specific anatomic sites. In this study, we describe the development of a novel chitosan nanobubble-based gene delivery system for ultrasound-triggered release. Methods and results: Chitosan was selected for the nanobubble shell because of its low toxicity, low immunogenicity, and excellent biocompatibility, while the core consisted of perfluoropentane. DNA-loaded chitosan nanobubbles were formed with a mean diameter of less than 300 nm and a positive surface charge. Transmission electron microscopic analysis confirmed composition of the core-shell structure. The ability of the chitosan nanobubbles to complex with and protect DNA was confirmed by agarose gel assay. Chitosan nanobubbles were found to be stable following insonation (2.5 MHz) for up to 3 minutes at 37°C. DNA release was evaluated in vitro in both the presence and absence of ultrasound. The release of chitosan nanobubble-bound plasmid DNA occurred after just one minute of insonation. In vitro transfection experiments were performed by exposing adherent COS7 cells to ultrasound in the presence of different concentrations of plasmid DNA-loaded nanobubbles. In the absence of ultrasound, nanobubbles failed to trigger transfection at all concentrations tested. In contrast, 30 seconds of ultrasound promoted a moderate degree of transfection. Cell viability experiments demonstrated that neither ultrasound nor the nanobubbles affected cell viability under these experimental conditions. Conclusion: Based on these results, chitosan nanobubbles have the potential to be promising tools for ultrasound-mediated DNA delivery.

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“…(Fig. 1B), whereas for the Colon26-Luc tumor, V min ranged from 4.8 mm 3 (D e ¼ 2.1mm) to V max ¼ 320 mm 3 (D e ¼ 8.5 mm; Fig. 1C).…”

Section: Resultsmentioning

confidence: 95%

“…The ALs were prepared as described previously (3,10). The shell composition was 1,2-distearoyl-sn-glycero-phosphatidylcholine (DSPC; NOF Co.) and N-(carbonyl-methoxypolyethyleneglycol-2000)-1,2-distearoyl-sn-glycero-3-phosphoethanolamine (DSPE-PEG2000-Ome; PEG molecular weight, 2,000; NOF Co.; 94:6 (mol/mol).…”

Section: Preparation Of Acoustic Liposomesmentioning

confidence: 99%

“…Acoustic liposomes (AL) are one kind of UCA and can add functionality along with echogenicity, serving as drug carriers with tissue specificity (3)(4)(5)(6). When their diameter is reduced to 100 to 200 nm, the enhanced permeability and retention (EPR) effect (7) can be exploited, creating the possibility of using high-frequency ultrasound (HF-US) to evaluate vascular permeability, vascular number, and vascular occupancy by their extravascular US imaging properties.…”

Section: Introductionmentioning

confidence: 99%

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Volumetric and Angiogenic Evaluation of Antitumor Effects with Acoustic Liposome and High-Frequency Ultrasound

et al. 2011

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Acoustic liposomes (AL) have their inherent echogenicity and can add functionality in serving as drug carriers with tissue specificity. Nonuniform vascular structures and vascular branches/bends are evaluated by imaging the intravascular movement locus of ALs with high-frequency ultrasound (HF-US) imaging. However, the evaluation of antitumor effects on angiogenesis by ALs and HF-US imaging has not been reported. Here, we show that the combination of ALs and an HF-US imaging system is capable of noninvasively evaluating antitumor volumetric and angiogenic effects in preclinical mouse models of various cancers. In this study, the antitumor effects of cisplatin on tumor growth and angiogenesis in mice bearing two different types of tumor cells were assessed. By tracking each AL flowing in the vessel and transferring the images to personal computers, microvessel structures were mapped and reconstructed using the color difference based on SD method. The antitumor effects were confirmed with an in vivo bioluminescence imaging system and immunohistochemical analysis. Our results show that cisplatin inhibits tumor growth by decreasing intratumoral vessel area but does not affect the angiogenesis ratio in the tumor. The vascular occupancy in the outer region of the tumor was larger than that in the inner region; however, both occupancies were similar to those of the control tumor. We propose that this method of mapping microvessels with ALs and an HF-US system can serve as a new molecular imaging method for the assessment of angiogenesis and can be applied to evaluate the antitumor effects by various therapeutic agents. Cancer Res; 71(22); 6957-64. Ó2011 AACR.

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Investigating the Effect of Polymeric Approaches on Circulation Time and Physical Properties of Nanobubbles

Abstract: This study identified the relevance of polymeric modifications to nanobubbles for an improved circulating lifetime, which would be essential for application of these systems in passive drug or gene targeting via the enhanced permeability and retention effect.

Cited by 32 publications

References 24 publications

Delivery of Molecules to the Lymph Node via Lymphatic Vessels Using Ultrasound and Nano/Microbubbles

Delivery of Molecules to the Lymph Node via Lymphatic Vessels Using Ultrasound and Nano/Microbubbles

New chitosan nanobubbles for ultrasound-mediated gene delivery: preparation and in vitro characterization

Volumetric and Angiogenic Evaluation of Antitumor Effects with Acoustic Liposome and High-Frequency Ultrasound

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