2024
DOI: 10.1021/acssynbio.4c00009
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Investigating the Effect of RNA Scaffolds on the Multicolor Fluorogenic Aptamer Pepper in Different Bacterial Species

Madeline M. Mumbleau,
Fabienne Chevance,
Kelly Hughes
et al.

Abstract: RNA synthetic biology tools have primarily been applied in E. coli; however, many other bacteria are of industrial and clinical significance. Thus, the multicolor fluorogenic aptamer Pepper was evaluated in both Grampositive and Gram-negative bacteria. Suitable HBC−Pepper dye pairs were identified that give blue, green, or red fluorescence signals in the E. coli, Bacillus subtilis, and Salmonella enterica serovar Typhimurium (S. Typhimurium). Furthermore, we found that different RNA scaffolds have a drastic ef… Show more

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