Investigating the effect of sugar-terminated nanoparticles on amyloid fibrillogenesis of β-lactoglobulin

Lin, Chien-Yu; Wang, Tzu-Horng; How, Su-Chun; Bednarikova, Zuzana; Fedunova, Diana; Gažová, Zuzana; Wu, Josephine W.; Wang, Steven S.‐S.

doi:10.1016/j.ijbiomac.2020.09.104

Cited by 11 publications

(2 citation statements)

References 126 publications

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“…After 8 h incubation at 90 °C and pH 2.0, the WPI sample exhibited large quantities of long, semi‐flexible and unbranched fibrils with a diameter of about 20 nm (Fig. 1G‐b), which was reminiscent of the structural features of typical amyloid fibrils (Lin et al ., 2020).…”

Section: Resultsmentioning

confidence: 99%

Preparation of a novel and stable iron fortifier: self‐assembled iron‐whey protein isolate fibrils nanocomposites

Zeng

Leng

Yang

et al. 2022

Int J of Food Sci Tech

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Iron deficiency anaemia (IDA) is a major global public health problem and iron fortification of foods has been recognised as the most cost-effective prevention strategy. However, traditional iron fortificants are easily oxidised and have poor colloidal stability. Herein, we took advantages of whey protein-isolated (WPI) amyloid fibrils and developed a stable and effective iron-WPI fibrils (Fe-FibWPI) nanocomposite for iron fortification. Compared with native WPI, the antioxidant activity of WPI fibrils was significantly improved after 8 h incubation at 90 °C and pH 2.0. Importantly, the degree of fibrillation and fibril concentration influenced iron reducibility. Therefore, we utilised WPI fibrils as anti-oxidising nanocarriers to form Fe-FibWPI nanocomposites. The Fe-FibWPI showed high dispersibility compared with iron nanoparticles (Fe-Nano). Further, the in vitro bioavailability of iron was significantly higher in the Fe-FibWPI at various storage conditions than that in the Fe-Nano. Moreover, the iron-WPI fibrils could be processed into liquid, gel and powder forms, which will broaden their application. Fe-FibWPI nanocomposites have the potential to develop as iron fortificants in IDA management.

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Section: Resultsmentioning

confidence: 99%

Preparation of a novel and stable iron fortifier: self‐assembled iron‐whey protein isolate fibrils nanocomposites

Zeng

Leng

Yang

et al. 2022

Int J of Food Sci Tech

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“…Recent data suggest that nanomaterials are potential inhibitors of amyloid formation and can be a therapeutic approach in amyloidosis. − We and others have previously demonstrated that appropriate particle surface functionalization allows nanomaterials to interact with proteins and block protein aggregation. , Porphyrins and their derivatives are excellent biological ligands. The most common examples are heme, chlorophylls, and bacteriochlorophylls.…”

Section: Introductionmentioning

confidence: 99%

Synthesis of Fe₃O₄-Chlorophyllin Nanoparticles for Preventing Amyloid Formation by Human Calcitonin

Chang

Hsu

et al. 2023

ACS Appl. Nano Mater.

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The accumulation of amyloid fibrils is crucial in the development of amyloidosis. In addition, some hormone peptides, such as human calcitonin (hCT), have limited therapeutic potential owing to a high tendency of amyloid formation. Nowadays, nanotechnology offers many advantages in various scientific fields and can overcome some of the limitations in the development of traditional therapeutics. In the present study, we applied iron and copper chlorophyllin in the synthesis of ironoxide nanoparticles to obtain porphyrin derivative-coated nanoparticles, namely, Fe 3 O 4 -Chl/Fe and Fe 3 O 4 -Chl/Cu, respectively. We observed that the two nanoparticles, especially Fe 3 O 4 -Chl/Fe, stabilized hCT and influenced hCT aggregation. To further confirm the applicability of Fe 3 O 4 -Chl/Fe, we utilized another benzothiazole-based fluorescent probe to monitor hCT amyloid formation. Several biophysical techniques, including transmission electron microscopy, dynamic light scattering, and isothermal titration calorimetry, were also implemented in our examinations. The fibrillar content of hCT after co-incubation was further determined using Bis-ANS and Nile red assays. The remaining soluble monomeric or oligomeric content of hCT was assessed by gel electrophoresis and biological assays. We believed that our findings would assist in the development of hCT drug formulation. Application of Fe 3 O 4 -Chl/Fe can increase the stability and bioactivity of hCT.

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Inhibiting protein aggregation with nanomaterials: The underlying mechanisms and impact factors

Wang

Zheng

et al. 2022

Biochimica et Biophysica Acta (BBA) - General Subjects

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Investigating the effect of sugar-terminated nanoparticles on amyloid fibrillogenesis of β-lactoglobulin

Cited by 11 publications

References 126 publications

Preparation of a novel and stable iron fortifier: self‐assembled iron‐whey protein isolate fibrils nanocomposites

Preparation of a novel and stable iron fortifier: self‐assembled iron‐whey protein isolate fibrils nanocomposites

Synthesis of Fe₃O₄-Chlorophyllin Nanoparticles for Preventing Amyloid Formation by Human Calcitonin

Inhibiting protein aggregation with nanomaterials: The underlying mechanisms and impact factors

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Investigating the effect of sugar-terminated nanoparticles on amyloid fibrillogenesis of β-lactoglobulin

Cited by 11 publications

References 126 publications

Preparation of a novel and stable iron fortifier: self‐assembled iron‐whey protein isolate fibrils nanocomposites

Preparation of a novel and stable iron fortifier: self‐assembled iron‐whey protein isolate fibrils nanocomposites

Synthesis of Fe3O4-Chlorophyllin Nanoparticles for Preventing Amyloid Formation by Human Calcitonin

Inhibiting protein aggregation with nanomaterials: The underlying mechanisms and impact factors

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Product

Resources

About

Synthesis of Fe₃O₄-Chlorophyllin Nanoparticles for Preventing Amyloid Formation by Human Calcitonin