Investigating the essential fatty acids in the common cuttlefish Sepia officinalis (Mollusca, Cephalopoda): Molecular cloning and functional characterisation of fatty acyl desaturase and elongase

Monroig, Óscar; Hontoria, Francisco; Varó, Inmaculada; Tocher, Douglas R.; Navarro, Juan Carlos

doi:10.1016/j.aquaculture.2015.07.003

Cited by 39 publications

(42 citation statements)

References 36 publications

(49 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Additionally, the P . lividus FadsA was confirmed to be a Δ5 desaturase, a desaturase ability reported in a variety of molluscs [10,12–14]. The present results demonstrate that P .…”

Section: Discussionsupporting

confidence: 79%

“…lividus desaturases showed high aa sequence identity, namely 81% (FadsA vs XP_783599.3), 85% (FadsC1 vs XP_783599.3), and 85% (FadsC2 vs XP_011683158.1). Moreover, FadsA clustered with Fads from several molluscs that have all been functionally characterised as Δ5 desaturases (Fig 3, clade A) [10,12–14]. In contrast to FadsA, the desaturases FadsC1, FadsC2 and Fads-like sequences from other sea urchin species uniquely formed a distinct clade from other Fads (Fig 3, clade C).…”

Section: Resultsmentioning

confidence: 98%

“…lividus Fads could potentially operate towards yeast endogenous saturated or monounsaturated FA, we further compared the FA profiles of yeast transformed with either pYES2-FadsA, pYES2-FadsC1 or pYES2-FadsC2 with that of yeast transformed with the empty pYES2 (control), and grown in all cases in the absence of exogenously added PUFA substrates. Each PUFA substrate was supplied to the yeast cultures in concentrations corresponding to 0.5 (C 18 ), 0.75 (C 20 ) and 1 mM (C 22 ) as uptake efficiency decreases with increasing chain length [12]. …”

Section: Methodsmentioning

confidence: 99%

“…Pioneer studies conducted in the common octopus Octopus vulgaris [10,11] and thereafter in the cephalopod Sepia officinalis [12], the gastropod Haliotis discus hannai [13] and the bivalve Chlamys nobilis [14–16] have confirmed that molluscs and potentially other marine invertebrates possess Fads and Elovl enzymes involved in the LC-PUFA biosynthesis. Additionally, the ability to synthesise non-methylene interrupted (NMI) FA, a group of PUFA with particular double bond distribution [17], was observed in several Fads enzymes from marine invertebrate species [10,12]. …”

Section: Introductionmentioning

confidence: 99%

“…These results further evidenced that the evolutionary history of genes involved in the LC-PUFA biosynthetic pathways of invertebrates, particularly the Fads-like desaturases, might differ markedly from those of vertebrates [6,12,19]. Clearly, marine invertebrates emerge as promising sources of LC-PUFA biosynthetic enzymes with potentially novel functionalities amenable for biotechnological production of n–3 oils [20].…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Biosynthesis of Polyunsaturated Fatty Acids in Sea Urchins: Molecular and Functional Characterisation of Three Fatty Acyl Desaturases from Paracentrotus lividus (Lamark 1816)

et al. 2017

View full text Add to dashboard Cite

Sea urchins are broadly recognised as a delicacy and their quality as food for humans is highly influenced by their diet. Lipids in general and the long-chain polyunsaturated fatty acids (LC-PUFA) in particular, are essential nutrients that determine not only the nutritional value of sea urchins but also guarantee normal growth and reproduction in captivity. The contribution of endogenous production (biosynthesis) of LC-PUFA in sea urchins remained unknown. Using Paracentrotus lividus as our model species, we aimed to characterise both molecularly and functionally the repertoire of fatty acyl desaturases (Fads), key enzymes in the biosynthesis of LC-PUFA, in sea urchins. Three Fads, namely FadsA, FadsC1 and FadsC2, were characterised. The phylogenetic analyses suggested that the repertoire of Fads within the Echinodermata phylum varies among classes. On one hand, orthologues of the P. lividus FadsA were found in other echinoderm classes including starfishes, brittle stars and sea cucumbers, thus suggesting that this desaturase is virtually present in all echinoderms. Contrarily, the FadsC appears to be sea urchin-specific desaturase. Finally, a further desaturase termed as FadsB exists in starfishes, brittle stars and sea cucumbers, but appears to be missing in sea urchins. The functional characterisation of the P. lividus Fads confirmed that the FadsA was a Δ5 desaturase with activity towards saturated and polyunsaturated fatty acids (FA). Moreover, our experiments confirmed that FadsA plays a role in the biosynthesis of non-methylene interrupted FA, a group of compounds typically found in marine invertebrates. On the other hand, both FadsC desaturases from P. lividus showed Δ8 activity. The present results demonstrate that P. lividus possesses desaturases that account for all the desaturation reactions required to biosynthesis the physiological essential eicosapentaenoic and arachidonic acids through the so-called “Δ8 pathway”.

show abstract

“…Additionally, the P . lividus FadsA was confirmed to be a Δ5 desaturase, a desaturase ability reported in a variety of molluscs [10,12–14]. The present results demonstrate that P .…”

Section: Discussionsupporting

confidence: 79%

Section: Resultsmentioning

confidence: 98%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Biosynthesis of Polyunsaturated Fatty Acids in Sea Urchins: Molecular and Functional Characterisation of Three Fatty Acyl Desaturases from Paracentrotus lividus (Lamark 1816)

et al. 2017

View full text Add to dashboard Cite

show abstract

Effects of temperature and salinity on the LC-PUFA biosynthesis and composition of the nereid polychaete Hediste diversicolor fed side streams

Villena-Rodríguez,

Monroig,

Hontoria

et al. 2024

Aquacult Int

View full text Add to dashboard Cite

The rapid growth of aquaculture has prompted the exploration of alternative and sustainable feed ingredients. Polychaetes, such as Hediste diversicolor, offer promise in the circular economy due to their ability to recycle waste materials from bioindustries and convert low nutritional value fatty acids (FA) into eicosapentaenoic (EPA) and arachidonic (ARA) acids. This study explored the combined effect of temperature and salinity on growth and LC-PUFA biosynthesis in H. diversicolor fed on a side stream diet (33% sludge from salmon aquaculture (AS) and 66% solid-phase digestate from biogas production (SBD)). Polychaetes were cultured for 28 days along a 5-step temperature and salinity gradient ranging from 7.7 to 17.9 °C and 5 to 40 psu, respectively. Growth was highest at high temperatures (≥ 14.3 °C) and salinities (≥ 35 psu). The FA composition remained relatively stable across different culture temperatures, while salinity exhibited higher levels of n − 3 PUFA and n − 3 LC-PUFA at lower salinities (5 and 15 psu) compared to higher salinity (35 psu), mainly driven by EPA. This suggests that salinity may exert a more pronounced effect than temperature on the FA profiles of H. diversicolor. Despite low temperature and high salinity (40 psu) significantly upregulating the expression of elongase and desaturase genes, this was not translated into higher LC-PUFA. Remarkably, polychaetes exhibited high levels of n − 3 and n − 6 LC-PUFA, even when fed a diet deficient in such FA. These findings highlight the potential of H. diversicolor for LC-PUFA biosynthesis, positioning this species as a promising candidate for producing high-nutritional-value ingredients for aquafeeds.

show abstract

Identification and Expression of PPAR in Sinonovacula constricta and Their Potential Regulatory Effects on Δ6 Fad Transcription

Ran

Kong

Liao

et al. 2021

J. Ocean Univ. China

View full text Add to dashboard Cite

Investigating the essential fatty acids in the common cuttlefish Sepia officinalis (Mollusca, Cephalopoda): Molecular cloning and functional characterisation of fatty acyl desaturase and elongase

Cited by 39 publications

References 36 publications

Biosynthesis of Polyunsaturated Fatty Acids in Sea Urchins: Molecular and Functional Characterisation of Three Fatty Acyl Desaturases from Paracentrotus lividus (Lamark 1816)

Biosynthesis of Polyunsaturated Fatty Acids in Sea Urchins: Molecular and Functional Characterisation of Three Fatty Acyl Desaturases from Paracentrotus lividus (Lamark 1816)

Effects of temperature and salinity on the LC-PUFA biosynthesis and composition of the nereid polychaete Hediste diversicolor fed side streams

Identification and Expression of PPAR in Sinonovacula constricta and Their Potential Regulatory Effects on Δ6 Fad Transcription

Contact Info

Product

Resources

About