A numerical investigation of mixed convection in a turbulent particle-laden channel flow is presented. Using Eulerian-Lagrangian Direct Numerical Simulations (DNSs) within the point-particle approach, the interaction between wall turbulence, particle inertia and thermal inertia, and buoyancy in the two-way coupling regime has been studied. The flow dynamics is controlled by the friction Reynolds number, Richardson number, particle Stokes and thermal Stokes numbers, and the Prandtl number. The effects of particle inertia and buoyancy on fluid and particle statistics are shown for a friction Reynolds number of 180, Stokes numbers ranging from 0.6 to 120, Richardson numbers ranging from 2.72 × 10−4 to 27.2 at a single Prandtl number, 0.71. The results indicate that the effect of particle inertia is significant on particle statistics, but not as pronounced on fluid statistics at the relatively low volume fraction considered. Particle inertia modulates the overall heat flux in a non-monotonic way.