Investigating the mechanism of aortic valve stenosis: the role of magnesium salts

Dritsa, V.; Pissaridi, Katerina; Anastassopoulou, J.; Koutoulakis, Emmanouil; Mamarelis, Ioannis; Kotoulas, Christophoros

doi:10.1186/1749-8090-8-s1-o18

Cited by 5 publications

(24 citation statements)

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“…This band is a "marker band", which also shows the progression of the disease. As it is shown (Figure 2), the intensity of this band increases considerably in the spectra of hyperuricaemic patients and it is associated with lipid peroxidation of the biological membranes [2,[23][24]. Our findings are in agreement with literature data, where it was observed in animal models during ischemia/reoxygenation [26].…”

Section: Region 1800-1500 CM -1supporting

confidence: 91%

“…Comparison between the spectra showed considerable intensity changes and frequency shifts in the entire spectral region. In the spectral region 4,000-3,000 cm -1 are found the bands that arise from B A indicating changes of the flexibility and permeability of membranes, which upon the disease became more lipophilic and more ordered [2,[23][24]. Deconvolution analysis of these bands shows a new band at 2,893 cm -1 , which is assigned to the branched alkyl chain.…”

Section: Spectral Region 4000-3000 CM -1mentioning

confidence: 99%

“…This band decreases in intensity and shifts to lower wavenumber at 1,633 cm -1 depending on the increase of serum uric acid. Deconvolution analysis showed three bands at: 1,691 cm -1 , 1,652 cm -1 and 1,625 cm -1 corresponding to antiparallel β-sheet, α-helix, random coil upon the disease development [23][24][25]. The β-sheet formation confirms the development of lipophilic environment, which is induced by the oxidation of membranes and amyloid like protein formation.…”

Section: Region 1800-1500 CM -1mentioning

confidence: 99%

“…After washing the biopsies in hexane, a non-polar solvent, it was found that the intensity decrease of the amide I and II bands was associated with the decrease of ApoI/ApoII ratio, which correspond to HDL and control LDL [27]. the stretching vibrations of vOH of water and saccharide molecules and vNH groups of proteins in the cell [14,15,23,24].…”

Section: Region 1800-1500 CM -1mentioning

confidence: 99%

“…The band at 3,060 cm -1 is assigned to stretching vibrations of the terminal olefinic v=CH mode, confirming the implication of oxidative stress during the disease development. This band's intensity was found to be proportional to LDL cholesterol concentration of the patients [2,[23][24].…”

Section: Region 1800-1500 CM -1mentioning

confidence: 99%

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The role of oxidative stress on molybdenum enzymes and ischemic reperfusion injury in hyperuricaemic patients. An infrared spectroscopic study

Mamareli¹,

Tanis²,

Anastassopoulou³

et al. 2019

Biomed Res Clin Prac

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There are many clinical evidences that hyperuricemia is a risk factor for the development of peripheral carotid and coronary vascular diseases. However, the mechanism of elevated uric acid concentration in biological systems is not yet clear. In the present work Fourier transform infrared (FT-IR) spectroscopy was used to evaluate the mechanism of calcification and plaque formation in carotid arteries in hyperuricaemic patients. Comparison between the spectra of carotid arteries from patients with elevated uric acid values and spectra obtained from patients with normal uric acid values showed structural changes of the characteristic spectral bands in the region 4000-500 cm -1 . These changes were related with changes in the concentration of the serum uric acid and the clinical history of the patients. The intensity decrease of the infrared bands in the region 1650-1500 cm -1 was associated with the decrease of the apolipoprotein ratio, ApoI/ApoII, which corresponds to HDL (High Density Lipoproteins) and the regulation of the LDL (Low Density Lipoproteins), which are related to oxidation stress. The infrared band at 1467 cm -1 indicated the presence of urea components as a result of the metabolic pathway. The shape and the intensity of the bands between 1250-900 cm -1 depend on the glycation-end products of the diseases. SEM-EDX chemical analysis showed fibril formation and molybdenum release in hyperuricaemic patients.

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Section: Region 1800-1500 CM -1supporting

confidence: 91%

Section: Spectral Region 4000-3000 CM -1mentioning

confidence: 99%

Section: Region 1800-1500 CM -1mentioning

confidence: 99%

Section: Region 1800-1500 CM -1mentioning

confidence: 99%

Section: Region 1800-1500 CM -1mentioning

confidence: 99%

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The role of oxidative stress on molybdenum enzymes and ischemic reperfusion injury in hyperuricaemic patients. An infrared spectroscopic study

Mamareli¹,

Tanis²,

Anastassopoulou³

et al. 2019

Biomed Res Clin Prac

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Raman Fingerprints of SARS-CoV-2 Omicron Subvariants: Molecular Roots of Virological Characteristics and Evolutionary Directions

Pezzotti,

Ohgitani,

Fujita

et al. 2023

ACS Infect. Dis.

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The latest RNA genomic mutation of SARS-CoV-2 virus, termed the Omicron variant, has generated a stream of highly contagious and antibody-resistant strains, which in turn led to classifying Omicron as a variant of concern. We systematically collected Raman spectra from six Omicron subvariants available in Japan (i.e., BA.1.18, BA.2, BA.4, BA.5, XE, and BA.2.75) and applied machine-learning algorithms to decrypt their structural characteristics at the molecular scale. Unique Raman fingerprints of sulfur-containing amino acid rotamers, RNA purines and pyrimidines, tyrosine phenol ring configurations, and secondary protein structures clearly differentiated the six Omicron subvariants. These spectral characteristics, which were linked to infectiousness, transmissibility, and propensity for immune evasion, revealed evolutionary motifs to be compared with the outputs of genomic studies. The availability of a Raman “metabolomic snapshot”, which was then translated into a barcode to enable a prompt subvariant identification, opened the way to rationalize in real-time SARS-CoV-2 activity and variability. As a proof of concept, we applied the Raman barcode procedure to a nasal swab sample retrieved from a SARS-CoV-2 patient and identified its Omicron subvariant by coupling a commercially available magnetic bead technology with our newly developed Raman analyses.

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Fourier transform infrared spectroscopic studies of radiation-induced molecular changes in bone and cartilage

Mavrogenis

Kyriakidou

Kyriazis

et al. 2016

Expert Review of Quality of Life in Cancer Care

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Investigating the mechanism of aortic valve stenosis: the role of magnesium salts

Cited by 5 publications

References 0 publications

The role of oxidative stress on molybdenum enzymes and ischemic reperfusion injury in hyperuricaemic patients. An infrared spectroscopic study

The role of oxidative stress on molybdenum enzymes and ischemic reperfusion injury in hyperuricaemic patients. An infrared spectroscopic study

Raman Fingerprints of SARS-CoV-2 Omicron Subvariants: Molecular Roots of Virological Characteristics and Evolutionary Directions

Fourier transform infrared spectroscopic studies of radiation-induced molecular changes in bone and cartilage

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