Investigating the Molecular Effects of Superovulation and Embryo Culture on Genomic Imprinting in a Mouse Model System.

Market-Velker, Brenna A.; Zhang, Liyue; Magri, Lauren S.; Bonvissuto, Anne C.; Mann, Mellissa R.W.

doi:10.1093/biolreprod/81.s1.266

Cited by 2 publications

(3 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The copyright holder for this preprint this version posted August 24, 2021. ; https://doi.org/10.1101/2021.08.23.457392 doi: bioRxiv preprint acquisition and maintenance of methylation marks on imprinted genes and fetal growth retardation all are associated with SOV protocols in cows and/or mice (Blondin et al, 1996;Van der Auwera, 2001;Fortier et al, 2008;Market-Velker et al, 2010). Differences in embryo development likely result from a lack of accumulation of other maternal components in the final period of follicle growth to the periovulatory stage, not related to the calcium signaling toolkit.…”

Section: Discussionmentioning

confidence: 99%

“…There is evidence in mice, cows, sheep and humans that superovulation has a detrimental impact on the quality of the resulting ovulated eggs or embryos (Moor et al, 1985;Hyttel et al, 1986;Yun et al, 1989;Assey et al, 1994;Blondin et al, 1996;Van der Auwera, 2001;Baart et al, 2007;Fortier et al, 2008;Market-Velker et al, 2010;Lee et al, 2017). These findings could be explained by many factors, including accelerated oocyte growth, rescue of abnormal oocytes from atresia, and recruitment for ovulation of non-mature oocytes, all of which could lead to eggs that lack the maternally derived stores needed for developmental competence.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Superovulation does not alter calcium oscillations following fertilization

Savy

Stein

Shi

et al. 2021

Preprint

View full text Add to dashboard Cite

Superovulation is a common approach to maximize the number of eggs available for either clinical assisted reproductive technologies or experimental animal studies. This procedure provides supraphysiological amounts of gonadotropins to promote continued growth and maturation of ovarian follicles that otherwise would undergo atresia. There is evidence in mice, cows, sheep and humans that superovulation has a detrimental impact on the quality of the resulting ovulated eggs or embryos. Here we tested the hypothesis that eggs derived from superovulation have a reduced capacity to support calcium oscillations following fertilization, which is a critical factor in the success of embryo development. Eggs were obtained from mice that were either naturally cycling or underwent a standard superovulation protocol. Naturally cycling mice were mated to vasectomized males and vaginal plugs were checked to assure ovulation had occurred. The superovulated mice were also mated to vasectomized males for consistency of treatment across groups. The eggs were fertilized in vitro while undergoing monitoring of calcium oscillatory patterns. There were no differences in any measures of calcium oscillatory behavior, including length of the first oscillation, area under the curve of calcium signal, or frequency or persistence of oscillations. These findings indicate that superovulation does not disrupt calcium signaling at fertilization, supporting the use of this method for both clinical and experimental purposes.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Superovulation does not alter calcium oscillations following fertilization

Savy

Stein

Shi

et al. 2021

Preprint

View full text Add to dashboard Cite

show abstract

“…Royster et al revealed that placenta accreta and placenta previa were more common in patients with E2 level > 3000 pg/mL on the day of the trigger (6). In addition, superovulation can alter the phenotype and fetal development by affecting the epigenetics of the oocyte by imprinted DNA methylation and histone post-translational modifications (7,8). Exclusion criteria were as follows: controlled ovarian stimulation using mild or natural cycle protocols, freeze-thaw cycles, patients with multiple embryo transfers, multiple pregnancies, preimplantation genetic diagnosis, oocyte and embryo recipients, patients with moderate to severe ovarian hyperstimula-tion syndrome (OHSS), oocyte retrieval of more than 15 and a history of chronic disease.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Estradiol on the HCG Trigger Day in Predicting Pregnancy and Neonatal Outcomes of Patients Undergoing Ivf/Icsi: A Retrospective Cohort Study

Erdoğan

Kahyaoğlu

Dilbaz

et al. 2022

Jinekoloji-Obstetrik Ve Neonatoloji Tıp Dergisi

View full text Add to dashboard Cite

Aim: Our aim was to investigate the value of serum estradiol (E2) on human chorionic gonadotrophin (hCG) trigger day in predicting pregnancy (ongoing pregnancy with live birth, abortion, iu ex, chemical pregnancy) outcomes and neonatal birth weight. Material-Methods: This retrospective study consisted of two groups: Group 1: Women who had live birth, labor after 22 gestational week (n= 417). Group 2: Women with clinical pregnancy: intrauterine ex, abortus, biochemical and anembryonic pregnancy (n=260). Demographic characteristics (maternal and paternal ages, body mass index (BMI), gravidity, abortion, and living child) were recorded. Clinical indications (male factor, diminished ovarian reserve...), duration of ovulation stimulation, day 3 (D3) E2, follicle stimulating hormone and luteinizing hormone levels, antral follicle count, total doses of stimulating agents, progesteron and estradiol levels on trigger day, number of oocytes retrieved, number of mature oocytes, endometrial thickness on trigger and oocyte pick-up day were compared between the two groups. Binary Logistic Regression, (Backward LR model) was used to determine the factors affecting the risk of not having a live birth. Results: Totally 677 pregnant women were included in the study. We found increased live birth rate in pregnant whom E2 level was higher on the trigger day. There was a statistically significant relation between E2 level on trigger day and newborn weight in live birth (p=0.005) It was determined that the male factor and age of the women are an important parameter that affect not having a live birth (p

show abstract

Investigating the Molecular Effects of Superovulation and Embryo Culture on Genomic Imprinting in a Mouse Model System.

Cited by 2 publications

References 0 publications

Superovulation does not alter calcium oscillations following fertilization

Superovulation does not alter calcium oscillations following fertilization

Evaluation of Estradiol on the HCG Trigger Day in Predicting Pregnancy and Neonatal Outcomes of Patients Undergoing Ivf/Icsi: A Retrospective Cohort Study

Contact Info

Product

Resources

About