Investigating the molecular structure of placenta and plasma in pre-eclampsia by infrared microspectroscopy

Dahabiyeh, Lina A.; Mansour, Randa S.H.; Saleh, Shawqi; Kamel, Gihan

doi:10.1016/j.jpba.2020.113186

Cited by 10 publications

(3 citation statements)

References 25 publications

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“…In our study, the median value of the 2924 cm −1 /2960 cm −1 ratio for the salivary glands is 3.45, whereas, for the gut, it is found to be 2.01 (Table 4). Although the peaks' assigned values are very close to each www.nature.com/scientificreports/ other, an increase in the ratio is found for the salivary gland's tissues, which may suggest conformational changes in the order of lipid acyl chains and the existence of long fatty acids 44,52 .…”

Section: Median Values Of Biochemical Parametersmentioning

confidence: 84%

Biochemical analysis of Hyalomma dromedarii salivary glands and gut tissues using SR-FTIR micro-spectroscopy

Hendawy,

Alzan,

Abdel-Ghany

et al. 2024

Sci Rep

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Ticks are obligatory voracious blood feeders infesting diverse vertebrate hosts, that have a crucial role in the transmission of diverse pathogens that threaten human and animal health. The continuous emergence of tick-borne diseases due to combined worldwide climatic changes, human activities, and acaricide-resistant tick strains, necessitates the development of novel ameliorative tick control strategies such as vaccines. The synchrotron-based Fourier transform infrared micro-spectroscopy (SR-FTIR) is a bioanalytical microprobe capable of exploring the molecular chemistry within microstructures at a cellular or subcellular level and is considered as a nondestructive analytical approach for biological specimens. In this study, SR-FTIR analysis was able to explore a qualitative and semi-quantitative biochemical composition of gut and salivary glands of Hyalomma dromedarii (H. dromedarii) tick detecting differences in the biochemical composition of both tissues. A notable observation regarding Amide I secondary structure protein profile was the higher ratio of aggregated strands in salivary gland and beta turns in gut tissues. Regarding the lipid profile, there was a higher intensity of lipid regions in gut tissue when compared to salivary glands. This detailed information on the biochemical compositions of tick tissues could assist in selecting vaccine and/or control candidates. Altogether, these findings confirmed SR-FTIR spectroscopy as a tool for detecting differences in the biochemical composition of H. dromedarii salivary glands and gut tissues. This approach could potentially be extended to the analysis of other ticks that are vectors of important diseases such as babesiosis and theileriosis.

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Section: Median Values Of Biochemical Parametersmentioning

confidence: 84%

Biochemical analysis of Hyalomma dromedarii salivary glands and gut tissues using SR-FTIR micro-spectroscopy

Hendawy,

Alzan,

Abdel-Ghany

et al. 2024

Sci Rep

Self Cite

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“…The models' quality was assessed by examining the goodness-of-fit parameter (R 2 X), the proportion of variance that the model explains (R 2 Y), and the model's predictive ability (Q 2 ) [76]. A Q 2 threshold of more than 0.5 was considered good predictability, while R 2 Y and Q 2 values close to 1 indicated satisfactory model performance [77,78]. The PLS-DA model was validated using default SIMCA 7fold cross-validation (CV) and permutation tests (200 permutations) upon obtaining a CV-ANOVA p-value threshold for significance less than 0.05 [45].…”

Section: Discussionmentioning

confidence: 99%

Identification and Quantitation of the Bioactive Components in Wasted Aralia elata Leaves Extract with Endothelial Protective Activity

Cho

Yang

et al. 2023

Molecules

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Aralia elata, a renowned medicinal plant with a rich history in traditional medicine, has gained attention for its potential therapeutic applications. However, the leaves of this plant have been largely overlooked and discarded due to limited knowledge of their biological activity and chemical composition. To bridge this gap, a comprehensive study was conducted to explore the therapeutic potential of the 70% ethanol extract derived from Aralia elata leaves (LAE) for the treatment of cardiovascular disease (CVD). Initially, the cytotoxic effects of LAE on human umbilical vein endothelial cells (HUVECs) were assessed, revealing no toxicity within concentrations up to 5 μg/mL. This suggests that LAE could serve as a safe raw material for the development of health supplements and drugs aimed at promoting cardiovascular well-being. Furthermore, the study found that LAE extract demonstrated anti-inflammatory properties in HUVECs by modulating the PI3K/Akt and MAPK signaling pathways. These findings are particularly significant as inflammation plays a crucial role in the progression of CVD. Moreover, LAE extract exhibited the ability to suppress the expression of adhesion molecules VCAM-1 and ICAM-1, which are pivotal in leukocyte migration to inflamed blood vessels observed in various pathological conditions. In conjunction with the investigation on therapeutic potential, the study also established an optimal HPLC–PDA–ESI–MS/MS method to identify and confirm the chemical constituents present in 24 samples collected from distinct regions in South Korea. Tentative identification revealed the presence of 14 saponins and nine phenolic compounds, while further analysis using PCA and PLS-DA allowed for the differentiation of samples based on their geographical origins. Notably, specific compounds such as chlorogenic acid, isochlorogenic acid A, and quercitrin emerged as marker compounds responsible for distinguishing samples from different regions. Overall, by unraveling its endothelial protective activity and identifying key chemical constituents, this research not only offers valuable insights for the development of novel treatments but also underscores the importance of utilizing and preserving natural resources efficiently.

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“…The collected raw spectra were processed using the multivariate statistical analysis software the Unscrambler X 10.4 (CAMO Analytics, Norway), which was used as previously described [ 48 , 49 , 50 ]. Briefly, the absorption spectra in the two identified spectral regions, lipid CH stretching (2800–3050 cm −1 ) and the protein (amide I and II)-carbonyl vibrations (1485–1855 cm −1 ), were baseline-corrected and smoothed with a 7-point window using the Savitzky–Golay smoothing algorithm (2 nd polynomial order).…”

Section: Methodsmentioning

confidence: 99%

Synchrotron-Radiation-Based Fourier Transform Infrared Microspectroscopy as a Tool for the Differentiation between Staphylococcal Small Colony Variants

et al. 2022

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Small colony variants (SCVs) are clinically significant and linked to persistent infections. In this study, synchrotron-radiation-based Fourier transform infrared (SR-FTIR) is used to investigate the microspectroscopic differences between the SCVs of Staphylococcus aureus (S. aureus) and diabetic foot Staphylococcus epidermidis (S. epidermidis) in two main IR spectral regions: (3050–2800 cm−1), corresponding to the distribution of lipids, and (1855–1500 cm−1), corresponding to the distribution of protein amide I and amide II and carbonyl vibrations. SR-FTIR successfully discriminated between the two staphylococcal species and between the SCV and the non-SCV strains within the two IR spectral regions. Combined S. aureus SCVs (SCVhMu) showed a higher protein content relative to the non-SCV wild type. Complemented S. aureus SCV showed distinguishable differences from the SCVhMu and the wild type, including a higher content of unsaturated fatty acids. An increase in the CH2/CH3 ratio was detected in S. epidermidis SCV samples compared to the standard control. Protein secondary structure in standard S. epidermidis and SCVs consisted mainly of an α-helix; however, a new shoulder at 1635 cm−1, assigned to β-sheets, was evident in the SCV. In conclusion, SR-FTIR is a powerful method that can discriminate between staphylococci species and to differentiate between SCVs and their corresponding natural strains.

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Investigating the molecular structure of placenta and plasma in pre-eclampsia by infrared microspectroscopy

Cited by 10 publications

References 25 publications

Biochemical analysis of Hyalomma dromedarii salivary glands and gut tissues using SR-FTIR micro-spectroscopy

Biochemical analysis of Hyalomma dromedarii salivary glands and gut tissues using SR-FTIR micro-spectroscopy

Identification and Quantitation of the Bioactive Components in Wasted Aralia elata Leaves Extract with Endothelial Protective Activity

Synchrotron-Radiation-Based Fourier Transform Infrared Microspectroscopy as a Tool for the Differentiation between Staphylococcal Small Colony Variants

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