Investigating the production of platelet lysate obtained from low volume Cord Blood Units: Focus on growth factor content and regenerative potential

Mallis, Panagiotis; Michalopoulos, Efstathios; Balampanis, Kostas; Sarri, Eirini-Faidra; Papadopoulou, Elena; Theodoropoulou, Vasiliki; Georgiou, Eleni; Kountouri, Aikaterini; Lambadiari, Vaia; Stavropoulos-Giokas, Catherine

doi:10.1016/j.transci.2022.103465

Cited by 5 publications

(10 citation statements)

References 37 publications

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“…However, CB-PL contained a significantly higher amount of the above proteins compared to the CB-PPP ( p < 0.001). The above results regarding the growth factor content seem to be aligned with those that have been described in the literature [ 18 , 19 , 24 , 41 ]. In the study of Valentini et al [ 24 ], comparable results regarding the levels of PDGF, VEGF, and FGF were presented.…”

Section: Discussionsupporting

confidence: 90%

“…However, these commercial products are limited to regenerative potential, due to the absence of wound healing mediators such as growth factors and tissue regenerative proteins [ 6 , 7 , 8 , 9 , 16 , 40 ]. Conversely, accumulating evidence from the literature indicated that CB derivatives are characterized by a great content of biomolecules [ 18 , 19 ]. For this reason, the CB products have been used with success in patients suffering from skin lesions and extended wounds [ 23 , 24 , 25 , 26 , 27 , 28 ].…”

Section: Discussionmentioning

confidence: 99%

“…To address this issue, the production of eye drops utilizing the blood derivatives such as the autologous serum has been proposed [ 17 ]. Allogeneic serum can resemble the natural composition of the tears, however, the serum has impaired regenerative potential, due to the low amount of growth factors [ 18 ]. Conversely, it has been proven that blood derivatives, such as PL, are rich in biomolecules, and thus can be potentially used as eye drops [ 18 , 19 ].…”

Section: Introductionmentioning

confidence: 99%

“…Allogeneic serum can resemble the natural composition of the tears, however, the serum has impaired regenerative potential, due to the low amount of growth factors [ 18 ]. Conversely, it has been proven that blood derivatives, such as PL, are rich in biomolecules, and thus can be potentially used as eye drops [ 18 , 19 ]. Indeed, platelets’ α-granules contain a significant amount of healing mediators and trophic factors, such as the platelet-derived growth factor (PDGF), transforming growth factor (TGF), fibroblast growth factor (FGF), hepatocyte growth factor (HGF), vascular endothelial growth factor (VEGF), etc.…”

Section: Introductionmentioning

confidence: 99%

“…Indeed, platelets’ α-granules contain a significant amount of healing mediators and trophic factors, such as the platelet-derived growth factor (PDGF), transforming growth factor (TGF), fibroblast growth factor (FGF), hepatocyte growth factor (HGF), vascular endothelial growth factor (VEGF), etc. [ 18 , 19 ]. It is known that the interplay between PDGF, FGF, TGF, and VEGF can promote the proliferation of cells and angiogenesis, resulting in advanced regeneration of the damaged tissue [ 20 , 21 , 22 ].…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of the Regenerative Potential of Platelet-Lysate and Platelet-Poor Plasma Derived from the Cord Blood Units in Corneal Wound Healing Applications: An In Vitro Comparative Study on Corneal Epithelial Cells

Mallis

Michalopoulos

Sarri

et al. 2022

CIMB

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Background: Cord blood platelet lysate (CB-PL) and cord blood platelet poor plasma (CB-PPP) have been applied with success in wound healing applications. Pathologies such as Sjogrens’s Syndrome (SS) and chronic graft versus host disease (cGVHD) can lead to severe ophthalmology issues. The application of CB-PL and CB-PPP may be strongly considered for damaged cornea healing. This study aimed to the evaluation of the beneficial properties of CB-PL and CB-PPP in corneal wound healing applications. Methods: Initially, the CB-PL and CB-PPP were produced from donated cord blood units (CBUs), followed by biochemical analysis. Corneal epithelial cells (CECs) were isolated from wistar rats and then cultured with medium containing 20% v/v either of CB-PL or CB-PPP. To define the impact of CB-PL and CB-PPP, biochemical, morphological analysis, scratch-wound assays, and immunoassays in CECs were performed. Results: CB-PL and CB-PPP were characterized by good biochemical parameters, regarding their quality characteristics and biomolecule content. CECs’ morphological features did not change after their cultivation with CB-PL or CB-PPP. A scratch wound assay and molecular analysis of CECs expanded with CB-PL indicated higher migratory capacity compared to those cultured with CB-PPP. Conclusion: CB-PL and CB-PPP exhibited good properties with respect to cell migration and proliferation, and could be considered an alternative source for eye drop production, to possibly be used in cornea wound healing applications.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Evaluation of the Regenerative Potential of Platelet-Lysate and Platelet-Poor Plasma Derived from the Cord Blood Units in Corneal Wound Healing Applications: An In Vitro Comparative Study on Corneal Epithelial Cells

Mallis

Michalopoulos

Sarri

et al. 2022

CIMB

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Rejuvenation of Ovarian Function after Autologous Platelet Lysate Injection: Promising Evidence from Confirmed Cases

Athanasios,

Efstathios,

Panagiotis

et al. 2023

Clin J Obstet Gynecol

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Intraovarian injection of autologous Platelet Lysate (PL) can be considered a potential therapeutic strategy for ovarian function rejuvenation. Especially, in women diagnosed with Poor Ovarian Response (POR) or Primary Ovarian Insufficiency (POI), the exogenous administration of the autologous platelet-derived growth factors, influence positively the regulation of the serum Follicle Stimulating Hormone (FSH), Luteinizing Hormone (LH), and Anti-Mullerian Hormone (AMH) and Estradiol (E2). Therefore, the evaluation of the serum levels of the aforementioned hormones was performed on 10 participants with a mean age of 43 ± 4 years diagnosed with POR or POI, who received intraovarian PL injection monotherapy. The monitoring of the serum hormone levels was performed for 3 months. The results of this study clearly showed that all participants were characterized by elevated levels of FSH and LH and reduced levels of E2 and AMH, prior to the PL injection. However, the levels of all hormones started to change after the 1st month of follow-up monitoring. Moreover, 40% of the participants conceived successfully either by natural way or after in vitro Fertilization (IVF). Considering these, the intraovarian injection of autologous PL exhibited promising evidence regarding the altering of hormone levels at physiological values. Moreover, the contained PL growth factors were implicated in angiogenesis promotion and also in toleration of the inflammatory microenvironment, regulating positively ovarian function. In conclusion, the intraovarian autologous PL injection is considered a safe, effective, and tolerable therapeutic strategy in women with POR or POI. Moreover, the results of this study were very encouraging, especially for the women with infertility issues, who want their genetic offspring.

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Effect of Helichrysum italicum in Promoting Collagen Deposition and Skin Regeneration in a New Dynamic Model of Skin Wound Healing

Serra,

Cruciani,

Garroni

et al. 2024

IJMS

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Natural products have many healing effects on the skin with minimal or no adverse effects. In this study, we analyzed the regenerative properties of a waste product (hydrolate) derived from Helichrysum italicum (HH) on scratch-tested skin cell populations seeded on a fluidic culture system. Helichrysum italicum has always been recognized in the traditional medicine of Mediterranean countries for its wide pharmacological activities. We recreated skin physiology with a bioreactor that mimics skin stem cell (SSCs) and fibroblast (HFF1) communication as in vivo skin layers. Dynamic culture models represent an essential instrument for recreating and preserving the complex multicellular organization and interactions of the cellular microenvironment. Both cell types were exposed to two different concentrations of HH after the scratch assay and were compared to untreated control cells. Collagen is the constituent of many wound care products that act directly on the damaged wound environment. We analyzed the role played by HH in stimulating collagen production during tissue repair, both in static and dynamic culture conditions, by a confocal microscopic analysis. In addition, we performed a gene expression analysis that revealed the activation of a molecular program of stemness in treated skin stem cells. Altogether, our results indicate a future translational application of this natural extract to support skin regeneration and define a new protocol to recreate a dynamic process of healing.

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Investigating the production of platelet lysate obtained from low volume Cord Blood Units: Focus on growth factor content and regenerative potential

Cited by 5 publications

References 37 publications

Evaluation of the Regenerative Potential of Platelet-Lysate and Platelet-Poor Plasma Derived from the Cord Blood Units in Corneal Wound Healing Applications: An In Vitro Comparative Study on Corneal Epithelial Cells

Evaluation of the Regenerative Potential of Platelet-Lysate and Platelet-Poor Plasma Derived from the Cord Blood Units in Corneal Wound Healing Applications: An In Vitro Comparative Study on Corneal Epithelial Cells

Rejuvenation of Ovarian Function after Autologous Platelet Lysate Injection: Promising Evidence from Confirmed Cases

Effect of Helichrysum italicum in Promoting Collagen Deposition and Skin Regeneration in a New Dynamic Model of Skin Wound Healing

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