Investigating the relationships between callus browning in Isatis indigotica Fortune, total phenol content, and PPO and POD activities

Su, Yong; Wei, Min; Q, Guo; Huang, Jiyue; Zhao, Ke; Huang, Jia-Bin

doi:10.1007/s11240-023-02567-7

Cited by 4 publications

(2 citation statements)

References 31 publications

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“…Specifically, enzyme activity can quickly reflect litter decomposition conditions and indirectly reflect the decomposition rate [34]. Peroxidase (POD) and polyphenol oxidase (PPO) are commonly found in nature and promote the conversion and degradation of phenolic substances through catalytic oxidation [35,36], which is closely related to the degradation of total phenols and condensed tannins [37].…”

Section: Introductionmentioning

confidence: 99%

Effects of Soil Fauna on the Home-Field Advantage of Litter Total Phenol and Condensed Tannin Decomposition

Lei,

Zeng,

Liu

et al. 2024

Forests

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Soil fauna play a vital role in contributing to the home-field advantage (HFA: litter decomposes faster in its natural habitat than elsewhere) during litter decomposition. Whether the presence of soil fauna affects the HFA of the decomposition of total phenols and condensed tannins, which are important components of litter, has rarely been investigated. In this study, litterbags with different mesh sizes were transplanted reciprocally, 0.04 mm (basically excluding soil fauna) and 3 mm (basically allowing all soil fauna to enter), in Lindera megaphylla and Cryptomeria fortunei forests. The results illustrated that the loss rates of total phenols and condensed tannins reached 64.07% to 84.49% and 69.67% to 88.37%, respectively, after 2 months of decomposition. Moreover, soil fauna positively contributed to the decomposition of condensed tannins in high-quality litter. After 2 months of decomposition, a significantly positive HFA (HFA index: 10.32) was found for total phenol decomposition in the coarse mesh, while a significantly negative HFA (HFA index: −1.81) was observed for condensed tannin decomposition in the fine mesh after 10 months of decomposition. Polyphenol oxidase (PPO) and peroxidase (POD) activities were significantly influenced by litter types. The loss rates of total phenols and condensed tannins were significantly negatively correlated with the initial N content, P content, N/P ratio, and POD activity and were positively related to the initial C content, total phenol content, condensed tannin content, C/P ratio, and C/N ratio. Only the loss of condensed tannins was negatively correlated with PPO activity (after 2 months’ decomposition). However, none of these correlations were observed after 10 months of decomposition. Our study illustrated that (1) soil fauna contributed to the decomposition of total phenols and condensed tannins but were influenced by litter type for condensed tannins. (2) The soil fauna had inconsistent effects on the HFA of total phenols and condensed tannins, possibly due to the combined regulatory effects of environmental context, litter quality, and rapid decomposition rates. In sum, the results indicated that soil fauna played an important role in the decomposition of condensed tannins and total phenols in litter, and additional studies on the effects of soil faunal abundance and class on HFA of condensed tannins and total phenols are needed.

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Section: Introductionmentioning

confidence: 99%

Effects of Soil Fauna on the Home-Field Advantage of Litter Total Phenol and Condensed Tannin Decomposition

Lei,

Zeng,

Liu

et al. 2024

Forests

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“…Excessive ROS levels generally exert a detrimental impact on explant growth [12]. Moreover, oxidases such as SOD, POD, and PPO also play substantial roles in callus formation and are closely associated with browning [13,14].…”

Section: Introductionmentioning

confidence: 99%

Effect of Nitric Oxide on Browning of Stem Tip Explants of Malus sieversii

Yang,

Liu,

Qin

et al. 2023

Horticulturae

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Browning is a major problem in the tissue culture of woody plants. Previous studies have shown that nitric oxide (NO) plays a role in regulating plant responses to stress, but its effect on browning in the tissue culture of Malus remains unclear. This study aimed to investigate the impact of exogenous NO donor sodium nitroprusside (SNP) on the browning of Malus sieversii stem tip explants. The results demonstrated that the addition of 50 μM SNP significantly reduced explant browning. Further analysis revealed that exogenous NO decreased the browning index (BD) and levels of malondialdehyde (MDA), hydrogen peroxide (H2O2), and superoxide anion radical O2−. Additionally, NO treatment increased the activity of antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT), and ascorbate peroxidase (APX). NO treatment also enhanced the activity of phenylalanine ammonia lyase (PAL), which contributed to the accumulation of flavonoids and inhibited the activity of polyphenol oxidase (PPO) and peroxidase (POD), which are key enzymes involved in the browning process. Furthermore, 50 μM SNP significantly promoted the accumulation of non-enzymatic antioxidants such as proline (Pro), soluble sugar (SS), and soluble protein (SP). Therefore, the results suggest that NO is able to counteract excessive reactive oxygen species (ROS) damage by enhancing both the enzymatic and non-enzymatic antioxidant systems, resulting in a reduction in browning in stem tip explants. Consequently, an improvement in the in vitro propagation efficiency of Malus sieversii shoot tip explants can be achieved.

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The efficacy of soaking fresh-cut nectarines in melatonin concentrations and its effect on antioxidant capacity microbial content and storage quality

Li,

Wang

et al. 2024

Scientia Horticulturae

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Investigating the relationships between callus browning in Isatis indigotica Fortune, total phenol content, and PPO and POD activities

Cited by 4 publications

References 31 publications

Effects of Soil Fauna on the Home-Field Advantage of Litter Total Phenol and Condensed Tannin Decomposition

Effects of Soil Fauna on the Home-Field Advantage of Litter Total Phenol and Condensed Tannin Decomposition

Effect of Nitric Oxide on Browning of Stem Tip Explants of Malus sieversii

The efficacy of soaking fresh-cut nectarines in melatonin concentrations and its effect on antioxidant capacity microbial content and storage quality

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