Investigating the role of Bowman-Birk serine protease inhibitor in Arabidopsis plants under drought stress

Malefo, M.B.; Mathibela, E.O.; Crampton, Bridget Genevieve; Makgopa, M.E.

doi:10.1016/j.plaphy.2020.02.007

Cited by 47 publications

(45 citation statements)

References 34 publications

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“…BBIs are likely involved in the development of salt [24] and drought [25] stress tolerance. The latter function may be associated with a decrease of drought-induced oxidative stress [26]. The higher concentration of two homologs BBIs (with 87% similarity in their amino acid sequences) has also been observed in rice under Fe-deficient conditions [27].…”

Section: Bowman-birk Inhibitors (Bbis)mentioning

confidence: 88%

Bowman-Birk Inhibitors: Insights into Family of Multifunctional Proteins and Peptides with Potential Therapeutical Applications

2020

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Bowman-Birk inhibitors (BBIs) are found primarily in seeds of legumes and in cereal grains. These canonical inhibitors share a highly conserved nine-amino acids binding loop motif CTP1SXPPXC (where P1 is the inhibitory active site, while X stands for various amino acids). They are natural controllers of plants’ endogenous proteases, but they are also inhibitors of exogenous proteases present in microbials and insects. They are considered as plants’ protective agents, as their elevated levels are observed during injury, presence of pathogens, or abiotic stress, i.a. Similar properties are observed for peptides isolated from amphibians’ skin containing 11-amino acids disulfide-bridged loop CWTP1SXPPXPC. They are classified as Bowman-Birk like trypsin inhibitors (BBLTIs). These inhibitors are resistant to proteolysis and not toxic, and they are reported to be beneficial in the treatment of various pathological states. In this review, we summarize up-to-date research results regarding BBIs’ and BBLTIs’ inhibitory activity, immunomodulatory and anti-inflammatory activity, antimicrobial and insecticidal strength, as well as chemopreventive properties.

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Section: Bowman-birk Inhibitors (Bbis)mentioning

confidence: 88%

Bowman-Birk Inhibitors: Insights into Family of Multifunctional Proteins and Peptides with Potential Therapeutical Applications

2020

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“…This gene only exhibited a significant up-regulation pattern under WH in DBA Aurora ( Table S6 ). A very recent study in Arabidopsis demonstrated that BBIs play important roles in stress adaptation via alleviating cellular oxidative stress [ 88 ]. Transgenic lines overexpressing the maize BBI gene showed significantly stronger trypsin-inhibitor activity, leading to enhanced antioxidant enzyme activities that contributed to the maintenance of higher leaf relative water content (RWC) under drought stress.…”

Section: Discussionmentioning

confidence: 99%

Integrated Analysis of Small RNA, Transcriptome, and Degradome Sequencing Reveals the Water-Deficit and Heat Stress Response Network in Durum Wheat

Liu

Able

2020

IJMS

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Water-deficit and heat stress negatively impact crop production. Mechanisms underlying the response of durum wheat to such stresses are not well understood. With the new durum wheat genome assembly, we conducted the first multi-omics analysis with next-generation sequencing, providing a comprehensive description of the durum wheat small RNAome (sRNAome), mRNA transcriptome, and degradome. Single and combined water-deficit and heat stress were applied to stress-tolerant and -sensitive Australian genotypes to study their response at multiple time-points during reproduction. Analysis of 120 sRNA libraries identified 523 microRNAs (miRNAs), of which 55 were novel. Differentially expressed miRNAs (DEMs) were identified that had significantly altered expression subject to stress type, genotype, and time-point. Transcriptome sequencing identified 49,436 genes, with differentially expressed genes (DEGs) linked to processes associated with hormone homeostasis, photosynthesis, and signaling. With the first durum wheat degradome report, over 100,000 transcript target sites were characterized, and new miRNA-mRNA regulatory pairs were discovered. Integrated omics analysis identified key miRNA-mRNA modules (particularly, novel pairs of miRNAs and transcription factors) with antagonistic regulatory patterns subject to different stresses. GO (Gene Ontology) and KEGG (Kyoto Encyclopedia of Genes and Genomes) enrichment analysis revealed significant roles in plant growth and stress adaptation. Our research provides novel and fundamental knowledge, at the whole-genome level, for transcriptional and post-transcriptional stress regulation in durum wheat.

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“…Bowman–Birk inhibitors (BBIs) are serine-type protease inhibitors important for plant biotic defense mechanisms against phytophagous insects and fungal and bacterial pathogens [ 39 ]. They are also described to play a role in abiotic stress responses to drought, heat, salt, oxidative stress and Fe-deficiency (for reviews, see [ 39 , 40 , 41 ].…”

Section: Discussionmentioning

confidence: 99%

Differentiation of the High Night Temperature Response in Leaf Segments of Rice Cultivars with Contrasting Tolerance

Schaarschmidt

Glaubitz

Erban

et al. 2021

IJMS

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High night temperatures (HNT) affect rice yield in the field and induce chlorosis symptoms in leaves in controlled chamber experiments. However, little is known about molecular changes in leaf segments under these conditions. Transcript and metabolite profiling were performed for leaf segments of six rice cultivars with different HNT sensitivity. The metabolite profile of the sheath revealed a lower metabolite abundance compared to segments of the leaf blade. Furthermore, pre-adaptation to stress under control conditions was detected in the sheath, whereas this segment was only slightly affected by HNT. No unique significant transcriptomic changes were observed in the leaf base, including the basal growth zone at HNT conditions. Instead, selected metabolites showed correlations with HNT sensitivity in the base. The middle part and the tip were most highly affected by HNT in sensitive cultivars on the transcriptomic level with higher expression of jasmonic acid signaling related genes, genes encoding enzymes involved in flavonoid metabolism and a gene encoding galactinol synthase. In addition, gene expression of expansins known to improve stress tolerance increased in tolerant and sensitive cultivars. The investigation of the different leaf segments indicated highly segment specific responses to HNT. Molecular key players for HNT sensitivity were identified.

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Investigating the role of Bowman-Birk serine protease inhibitor in Arabidopsis plants under drought stress

Cited by 47 publications

References 34 publications

Bowman-Birk Inhibitors: Insights into Family of Multifunctional Proteins and Peptides with Potential Therapeutical Applications

Bowman-Birk Inhibitors: Insights into Family of Multifunctional Proteins and Peptides with Potential Therapeutical Applications

Integrated Analysis of Small RNA, Transcriptome, and Degradome Sequencing Reveals the Water-Deficit and Heat Stress Response Network in Durum Wheat

Differentiation of the High Night Temperature Response in Leaf Segments of Rice Cultivars with Contrasting Tolerance

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